Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Combinatorial selection of a single stranded DNA thioaptamer targeting TGF-beta1 protein.

Literature DB >> 18294846

Combinatorial selection of a single stranded DNA thioaptamer targeting TGF-beta1 protein.

Jonghoon Kang¹, Myung Soog Lee, John A Copland, Bruce A Luxon, David G Gorenstein.

Abstract

A phosphorothioate single-stranded DNA aptamer (thioaptamer) targeting transforming growth factor-beta1 (TGF-beta1) was isolated by in-vitro combinatorial selection. The aptamer selection procedure was designed to modify the backbone of single-stranded DNA aptamers, where 5' of both A and C are phosphorothioates, since this provides enhanced nuclease resistance as well as higher affinity than that of a phosphate counterpart. The thioaptamer selected from a combinatorial library (5x10(14) sequences) binds to TGF-beta1 protein with an affinity of 90 nM. In this report, sequence, predicted secondary structure, and binding affinity of the selected thioaptamer (T18_1_3) are presented.

Entities: Chemical Gene

Mesh：

Substances：

Year: 2008 PMID： 18294846 PMCID： PMC2323357 DOI： 10.1016/j.bmcl.2008.02.023

Source DB: PubMed Journal: Bioorg Med Chem Lett ISSN： 0960-894X Impact factor: 2.823

22 in total

1. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.

Authors: C Tuerk; L Gold
Journal: Science Date: 1990-08-03 Impact factor: 47.728

2. Chemiluminescence-based electrophoretic mobility shift assay of RNA-protein interactions: application to binding of viral capsid proteins to RNA.

Authors: Jonghoon Kang; Myung Soog Lee; Stanley J Watowich; David G Gorenstein
Journal: J Virol Methods Date: 2005-09-22 Impact factor: 2.014

3. Quantitative analysis of chemiluminescence signals using a cooled charge-coupled device camera.

Authors: Jonghoon Kang; Myung Soog Lee; David G Gorenstein
Journal: Anal Biochem Date: 2005-10-01 Impact factor: 3.365

4. Chemiluminescence-based electrophoretic mobility shift assay of heparin-protein interactions.

Authors: Jonghoon Kang; Myung Soog Lee; David G Gorenstein
Journal: Anal Biochem Date: 2005-11-30 Impact factor: 3.365

5. In vitro selection of RNA molecules that bind specific ligands.

Authors: A D Ellington; J W Szostak
Journal: Nature Date: 1990-08-30 Impact factor: 49.962

6. Novel combinatorial selection of phosphorothioate oligonucleotide aptamers.

Authors: D J King; D A Ventura; A R Brasier; D G Gorenstein
Journal: Biochemistry Date: 1998-11-24 Impact factor: 3.162

Review 7. Role of transforming growth factor Beta in human cancer.

Authors: Rebecca L Elliott; Gerard C Blobe
Journal: J Clin Oncol Date: 2005-03-20 Impact factor: 44.544

Review 8. Progress in thioaptamer development.

Authors: Xianbin Yang; David G Gorenstein
Journal: Curr Drug Targets Date: 2004-11 Impact factor: 3.465

9. Determination of RNA-protein contacts using thiophosphate substitutions.

Authors: J F Milligan; O C Uhlenbeck
Journal: Biochemistry Date: 1989-04-04 Impact factor: 3.162

10. In vitro selection of phosphorothiolated aptamers.

Authors: S Jhaveri; B Olwin; A D Ellington
Journal: Bioorg Med Chem Lett Date: 1998-09-08 Impact factor: 2.823

25 in total

Review 1. Aptamers in immunotherapy.

Authors: Claudia M Dollins; Smita Nair; Bruce A Sullenger
Journal: Hum Gene Ther Date: 2008-05 Impact factor: 5.695

Combinatorial selection of a single stranded DNA thioaptamer targeting TGF-beta1 protein.

1. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.

2. Chemiluminescence-based electrophoretic mobility shift assay of RNA-protein interactions: application to binding of viral capsid proteins to RNA.

3. Quantitative analysis of chemiluminescence signals using a cooled charge-coupled device camera.

4. Chemiluminescence-based electrophoretic mobility shift assay of heparin-protein interactions.

5. In vitro selection of RNA molecules that bind specific ligands.

6. Novel combinatorial selection of phosphorothioate oligonucleotide aptamers.

Review 7. Role of transforming growth factor Beta in human cancer.

Review 8. Progress in thioaptamer development.

9. Determination of RNA-protein contacts using thiophosphate substitutions.

10. In vitro selection of phosphorothiolated aptamers.

Review 1. Aptamers in immunotherapy.

Review 2. Non-natural nucleic acids for synthetic biology.

3. Strategies for the discovery of therapeutic aptamers.

4. Thioaptamers targeting dengue virus type-2 envelope protein domain III.

Review 5. Recent Advances in Aptamers Targeting Immune System.

Review 6. Aptamers Against Pro- and Anti-Inflammatory Cytokines: A Review.

Review 7. Manipulating the in vivo immune response by targeted gene knockdown.

Review 8. Design of growth factor sequestering biomaterials.

Review 9. Aptamers and the next generation of diagnostic reagents.

Review 10. Nanomedicine--challenge and perspectives.