Literature DB >> 24253829

A rapid screening technique for the detection of repeated DNA sequences in plant tissues.

J Hutchinson1, A Abbott, M O Dell, R B Flavell.   

Abstract

DNA sequences cloned from nuclear and mitochondrial chromosomes have been used as hybridisation probes to distinguish different plant genotypes. The probes are hybridised to squashed segments of tissue e.g. root tips. The 'squash-dot' method is rapid and suitable for screening large numbers of individual plants. One probe, specific for a rye repeated sequence family, enables rye chromosomes to be detected in wheat plants. A probe for ribosomal DNA enables plants with high or low numbers of ribosomal RNA genes to be distinguished. A maize mitochondrial DNA probe is used to distinguish plants with N, T or S cytoplasms.

Entities:  

Year:  1985        PMID： 24253829     DOI： 10.1007/BF00662455

Source DB:  PubMed          Journal:  Theor Appl Genet        ISSN： 0040-5752            Impact factor:   5.699


  16 in total

1.  Filter replicas and permanent collections of recombinant DNA plasmids.

Authors:  J P Gergen; R H Stern; P C Wensink
Journal:  Nucleic Acids Res       Date:  1979-12-20       Impact factor: 16.971

2.  Unique DNA associated with mitochondria in the "S"-type cytoplasm of male-sterile maize.

Authors:  D R Pring; C S Levings; W W Hu; D H Timothy
Journal:  Proc Natl Acad Sci U S A       Date:  1977-07       Impact factor: 11.205

3.  Sensitive and rapid diagnosis of potato spindle tuber viroid disease by nucleic Acid hybridization.

Authors:  R A Owens; T O Diener
Journal:  Science       Date:  1981-08-07       Impact factor: 47.728

4.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

5.  The role of homoeologous group 1 chromosomes in the control of rRNA genes in wheat.

Authors:  R B Flavell; D B Smith
Journal:  Biochem Genet       Date:  1974-10       Impact factor: 1.890

6.  The integrated forms of the S1 and S2 DNA elements of maize male sterile mitochondrial DNA are flanked by a large repeated sequence.

Authors:  D M Lonsdale; R D Thompson; T P Hodge
Journal:  Nucleic Acids Res       Date:  1981-08-11       Impact factor: 16.971

7.  The application of spot hybridization to the detection of DNA and RNA viruses in plant tissues.

Authors:  A J Maule; R Hull; J Donson
Journal:  J Virol Methods       Date:  1983-04       Impact factor: 2.014

8.  A membrane-filter technique for the detection of complementary DNA.

Authors:  D T Denhardt
Journal:  Biochem Biophys Res Commun       Date:  1966-06-13       Impact factor: 3.575

9.  Cloning and characterization of ribosomal RNA genes from wheat and barley.

Authors:  W L Gerlach; J R Bedbrook
Journal:  Nucleic Acids Res       Date:  1979-12-11       Impact factor: 16.971

10.  Nucleotide sequence of the rightward operator of phage lambda.

Authors:  T Maniatis; A Jeffrey; D G Kleid
Journal:  Proc Natl Acad Sci U S A       Date:  1975-03       Impact factor: 11.205
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  7 in total

1.  Isolation and characterization of wheat-rye recombinants involving chromosome arm 1DS of wheat.

Authors:  P M Rogowsky; F L Guidet; P Langridge; K W Shepherd; R M Koebner
Journal:  Theor Appl Genet       Date:  1991-10       Impact factor: 5.699

2.  Selection of monosomic addition plants in offspring families using repetitive DNA probes in Beta L.

Authors:  M Mesbah; T S De Bock; J M Sandbrink; R M Klein-Lankhorst; W Lange
Journal:  Theor Appl Genet       Date:  1996-05       Impact factor: 5.699

3.  Discrimination between closely related Triticeae species using genomic DNA as a probe.

Authors:  K Anamthawat-Jónsson; T Schwarzacher; A R Leitch; M D Bennett; J S Heslop-Harrison
Journal:  Theor Appl Genet       Date:  1990-06       Impact factor: 5.699

4.  FISH to mitotic chromosomes and extended DNA fibres of Beta procumbens in a series of monosomic additions to beet (B. vulgaris).

Authors:  M Mesbah; J Wennekes-Van Eden; J H De Jong; T S De Bock; W Lange
Journal:  Chromosome Res       Date:  2000       Impact factor: 5.239

5.  Genome specific, highly repeated sequences of Hordeum vulgare: cloning, sequencing and squash dot test.

Authors:  H Junghans; M Metzlaff
Journal:  Theor Appl Genet       Date:  1988-11       Impact factor: 5.699

6.  Construction of Beta procumbens-specific DNA probes and their application for the screening of B. vulgaris x B. procumbens (2n = 19) addition lines.

Authors:  T Schmidt; H Junghans; M Metzlaff
Journal:  Theor Appl Genet       Date:  1990-02       Impact factor: 5.699

7.  Hordeum chilense repetitive sequences. Genome characterization using biotinylated probes.

Authors:  G Hueros; J V Monte; E Ferrer
Journal:  Theor Appl Genet       Date:  1990-07       Impact factor: 5.699
  7 in total

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