| Literature DB >> 17555838 |
Seiya Watanabe1, Ahmed Abu Saleh, Seung Pil Pack, Narayana Annaluru, Tsutomu Kodaki, Keisuke Makino.
Abstract
Effects of reversal coenzyme specificity toward NADP+ and thermostabilization of xylitol dehydrogenase (XDH) from Pichia stipitis on fermentation of xylose to ethanol were estimated using a recombinant Saccharomyces cerevisiae expressing together with a native xylose reductase from P. stipitis. The mutated XDHs performed the similar enzyme properties in S. cerevisiae cells, compared with those in vitro. The significant enhancement(s) was found in Y-ARSdR strain, in which NADP+-dependent XDH was expressed; 86% decrease of unfavorable xylitol excretion with 41% increased ethanol production, when compared with the reference strain expressing the wild-type XDH.Entities:
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Year: 2007 PMID： 17555838 DOI： 10.1016/j.jbiotec.2007.04.019
Source DB: PubMed Journal: J Biotechnol ISSN： 0168-1656 Impact factor: 3.307