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Literature DB >> 17519204

Single-molecule biophysics: at the interface of biology, physics and chemistry.

Ashok A Deniz¹, Samrat Mukhopadhyay, Edward A Lemke.

Abstract

Single-molecule methods have matured into powerful and popular tools to probe the complex behaviour of biological molecules, due to their unique abilities to probe molecular structure, dynamics and function, unhindered by the averaging inherent in ensemble experiments. This review presents an overview of the burgeoning field of single-molecule biophysics, discussing key highlights and selected examples from its genesis to our projections for its future. Following brief introductions to a few popular single-molecule fluorescence and manipulation methods, we discuss novel insights gained from single-molecule studies in key biological areas ranging from biological folding to experiments performed in vivo.

Mesh：

Substances：
Molecular Motor Proteins

Year: 2008 PMID： 17519204 PMCID： PMC2094721 DOI： 10.1098/rsif.2007.1021

Source DB: PubMed Journal: J R Soc Interface ISSN： 1742-5662 Impact factor: 4.118

289 in total

1. Fluorescence intensity multiple distributions analysis: concurrent determination of diffusion times and molecular brightness.

Authors: K Palo; U Mets; S Jäger; P Kask; K Gall
Journal: Biophys J Date: 2000-12 Impact factor: 4.033

2. Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking.

Authors: Maxime Dahan; Sabine Lévi; Camilla Luccardini; Philippe Rostaing; Béatrice Riveau; Antoine Triller
Journal: Science Date: 2003-10-17 Impact factor: 47.728

3. Exploration of the transition state for tertiary structure formation between an RNA helix and a large structured RNA.

Authors: Laura E Bartley; Xiaowei Zhuang; Rhiju Das; Steven Chu; Daniel Herschlag
Journal: J Mol Biol Date: 2003-05-16 Impact factor: 5.469

Review 4. Chromatin fibers, one-at-a-time.

Authors: Jordanka Zlatanova; Sanford H Leuba
Journal: J Mol Biol Date: 2003-08-01 Impact factor: 5.469

5. Imaging of single-molecule translocation through nuclear pore complexes.

Authors: Weidong Yang; Jeff Gelles; Siegfried M Musser
Journal: Proc Natl Acad Sci U S A Date: 2004-08-11 Impact factor: 11.205

6. Rapid spontaneous accessibility of nucleosomal DNA.

Authors: Gu Li; Marcia Levitus; Carlos Bustamante; Jonathan Widom
Journal: Nat Struct Mol Biol Date: 2004-12-05 Impact factor: 15.369

7. Mechanism of force generation of a viral DNA packaging motor.

Authors: Yann R Chemla; K Aathavan; Jens Michaelis; Shelley Grimes; Paul J Jardine; Dwight L Anderson; Carlos Bustamante
Journal: Cell Date: 2005-09-09 Impact factor: 41.582

8. Many expressed genes in bacteria and yeast are transcribed only once per cell cycle.

Authors: Michaël Bon; Simon J McGowan; Peter R Cook
Journal: FASEB J Date: 2006-07-03 Impact factor: 5.191

9. Nucleophilic catalysis of oxime ligation.

Authors: Anouk Dirksen; Tilman M Hackeng; Philip E Dawson
Journal: Angew Chem Int Ed Engl Date: 2006-11-20 Impact factor: 15.336

Review 10. Kinesin motor mechanics: binding, stepping, tracking, gating, and limping.

Authors: Steven M Block
Journal: Biophys J Date: 2007-02-26 Impact factor: 4.033

74 in total

1. Massively parallel single-molecule manipulation using centrifugal force.

Authors: Ken Halvorsen; Wesley P Wong
Journal: Biophys J Date: 2010-06-02 Impact factor: 4.033

Review 2. The applications of atomic force microscopy to vision science.

Authors: Julie A Last; Paul Russell; Paul F Nealey; Christopher J Murphy
Journal: Invest Ophthalmol Vis Sci Date: 2010-12 Impact factor: 4.799

3. Exploring both sequence detection and restriction endonuclease cleavage kinetics by recognition site via single-molecule microfluidic trapping.

Authors: Weilin Xu; Susan J Muller
Journal: Lab Chip Date: 2010-11-12 Impact factor: 6.799

Single-molecule biophysics: at the interface of biology, physics and chemistry.

1. Fluorescence intensity multiple distributions analysis: concurrent determination of diffusion times and molecular brightness.

2. Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking.

3. Exploration of the transition state for tertiary structure formation between an RNA helix and a large structured RNA.

Review 4. Chromatin fibers, one-at-a-time.

5. Imaging of single-molecule translocation through nuclear pore complexes.

6. Rapid spontaneous accessibility of nucleosomal DNA.

7. Mechanism of force generation of a viral DNA packaging motor.

8. Many expressed genes in bacteria and yeast are transcribed only once per cell cycle.

9. Nucleophilic catalysis of oxime ligation.

Review 10. Kinesin motor mechanics: binding, stepping, tracking, gating, and limping.

1. Massively parallel single-molecule manipulation using centrifugal force.

Review 2. The applications of atomic force microscopy to vision science.

3. Exploring both sequence detection and restriction endonuclease cleavage kinetics by recognition site via single-molecule microfluidic trapping.

Review 4. Fluorescence from diffusing single molecules illuminates biomolecular structure and dynamics.

5. Direct single-molecule observation of a protein living in two opposed native structures.

6. Allosteric inhibition of individual enzyme molecules trapped in lipid vesicles.

Review 7. Single-molecule fluorescence studies of intrinsically disordered proteins and liquid phase separation.

8. Interplay of alpha-synuclein binding and conformational switching probed by single-molecule fluorescence.

9. Microfluidic device for single-molecule experiments with enhanced photostability.

10. Improved Glass Surface Passivation for Single-Molecule Nanoarrays.