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Literature DB >> 19772358

Microfluidic device for single-molecule experiments with enhanced photostability.

Edward A Lemke¹, Yann Gambin, Virginia Vandelinder, Eric M Brustad, Hsiao-Wei Liu, Peter G Schultz, Alex Groisman, Ashok A Deniz.

Abstract

A microfluidic device made of polydimethylsiloxane (PDMS) addresses key limitations in single-molecule fluorescence experiments by providing high dye photostability and low sample sticking. Photobleaching is dramatically reduced by deoxygenation via gas diffusion through porous channel walls. Rapid buffer exchange in a laminar sheath flow followed by optical interrogation minimizes surface-sample contacts and allows the in situ addition and combination of other reagents.

Entities: Chemical Disease Gene Species

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Year: 2009 PMID： 19772358 PMCID： PMC2779842 DOI： 10.1021/ja9027023

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

18 in total

1. Three-color single-molecule fluorescence resonance energy transfer.

Authors: Jean-Pierre Clamme; Ashok A Deniz
Journal: Chemphyschem Date: 2005-01 Impact factor: 3.102

2. Development of an integrated microfluidic platform for dynamic oxygen sensing and delivery in a flowing medium.

Authors: Adam P Vollmer; Ronald F Probstein; Richard Gilbert; Todd Thorsen
Journal: Lab Chip Date: 2005-08-25 Impact factor: 6.799

3. Major signal increase in fluorescence microscopy through dark-state relaxation.

Authors: Gerald Donnert; Christian Eggeling; Stefan W Hell
Journal: Nat Methods Date: 2006-12-10 Impact factor: 28.547

4. Nonblinking and long-lasting single-molecule fluorescence imaging.

Authors: Ivan Rasnik; Sean A McKinney; Taekjip Ha
Journal: Nat Methods Date: 2006-10-01 Impact factor: 28.547

5. Single-molecule measurement of protein folding kinetics.

Authors: Everett A Lipman; Benjamin Schuler; Olgica Bakajin; William A Eaton
Journal: Science Date: 2003-08-29 Impact factor: 47.728

6. Analysis of photobleaching in single-molecule multicolor excitation and Förster resonance energy transfer measurements.

Authors: Christian Eggeling; Jerker Widengren; Leif Brand; Jörg Schaffer; Suren Felekyan; Claus A M Seidel
Journal: J Phys Chem A Date: 2006-03-09 Impact factor: 2.781

7. Optical imaging in microfluidic bioreactors enables oxygen monitoring for continuous cell culture.

Authors: Dhruv Sud; Geeta Mehta; Khamir Mehta; Jennifer Linderman; Shuichi Takayama; Mary-Ann Mycek
Journal: J Biomed Opt Date: 2006 Sep-Oct Impact factor: 3.170

8. Nonequilibrium single molecule protein folding in a coaxial mixer.

Authors: Kambiz M Hamadani; Shimon Weiss
Journal: Biophys J Date: 2008-03-13 Impact factor: 4.033

9. A general and efficient method for the site-specific dual-labeling of proteins for single molecule fluorescence resonance energy transfer.

Authors: Eric M Brustad; Edward A Lemke; Peter G Schultz; Ashok A Deniz
Journal: J Am Chem Soc Date: 2008-12-31 Impact factor: 15.419

10. Single-molecule photophysics of oxazines on DNA and its application in a FRET switch.

Authors: Jan Vogelsang; Thorben Cordes; Philip Tinnefeld
Journal: Photochem Photobiol Sci Date: 2009-03-12 Impact factor: 3.982

27 in total

1. Single-molecule spectroscopy of protein folding in a chaperonin cage.

Authors: Hagen Hofmann; Frank Hillger; Shawn H Pfeil; Armin Hoffmann; Daniel Streich; Dominik Haenni; Daniel Nettels; Everett A Lipman; Benjamin Schuler
Journal: Proc Natl Acad Sci U S A Date: 2010-06-14 Impact factor: 11.205

2. Linear conversion of pressure into concentration, rapid switching of concentration, and generation of linear ramps of concentration in a microfluidic device.

Authors: Micha Adler; Alex Groisman
Journal: Biomicrofluidics Date: 2012-04-13 Impact factor: 2.800

3. Microfluidic mixer designed for performing single-molecule kinetics with confocal detection on timescales from milliseconds to minutes.

Authors: Bengt Wunderlich; Daniel Nettels; Stephan Benke; Jennifer Clark; Sascha Weidner; Hagen Hofmann; Shawn H Pfeil; Benjamin Schuler
Journal: Nat Protoc Date: 2013-07-11 Impact factor: 13.491

Review 4. Ultra-stable organic fluorophores for single-molecule research.

Authors: Qinsi Zheng; Manuel F Juette; Steffen Jockusch; Michael R Wasserman; Zhou Zhou; Roger B Altman; Scott C Blanchard
Journal: Chem Soc Rev Date: 2014-02-21 Impact factor: 54.564

5. Four-color alternating-laser excitation single-molecule fluorescence spectroscopy for next-generation biodetection assays.

Authors: Seok W Yim; Taiho Kim; Ted A Laurence; Steve Partono; Dongsik Kim; Younggyu Kim; Shimon Weiss; Armin Reitmair
Journal: Clin Chem Date: 2012-01-19 Impact factor: 8.327

9. Acidification of the oxygen scavenging system in single-molecule fluorescence studies: in situ sensing with a ratiometric dual-emission probe.

Authors: Xinghua Shi; John Lim; Taekjip Ha
Journal: Anal Chem Date: 2010-07-15 Impact factor: 6.986

10. Superhydrophobic photosensitizers. Mechanistic studies of (1)O2 generation in the plastron and solid/liquid droplet interface.

Authors: David Aebisher; Dorota Bartusik; Yang Liu; Yuanyuan Zhao; Mark Barahman; QianFeng Xu; Alan M Lyons; Alexander Greer
Journal: J Am Chem Soc Date: 2013-12-10 Impact factor: 15.419