Literature DB >> 17088371

Rapid identification of pathogens in blood cultures with a modified fluorescence in situ hybridization assay.

Remco P H Peters1, Michiel A van Agtmael, Alberdina M Simoons-Smit, Sven A Danner, Christina M J E Vandenbroucke-Grauls, Paul H M Savelkoul.   

Abstract

We evaluated a modified fluorescence in situ hybridization (FISH) assay for rapid (<1 h) identification of microorganisms in growth-positive blood cultures. The results were compared to those of the standard FISH technique and conventional culturing. The rapid identification of microorganisms with modified FISH can have important effects on clinical management of patients with bloodstream infections.

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Year:  2006        PMID: 17088371      PMCID: PMC1698318          DOI: 10.1128/JCM.01085-06

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  15 in total

1.  Detection and treatment of bloodstream infection: laboratory reporting and antimicrobial management.

Authors:  Erik L Munson; Daniel J Diekema; Susan E Beekmann; Kimberle C Chapin; Gary V Doern
Journal:  J Clin Microbiol       Date:  2003-01       Impact factor: 5.948

2.  Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations.

Authors:  R I Amann; B J Binder; R J Olson; S W Chisholm; R Devereux; D A Stahl
Journal:  Appl Environ Microbiol       Date:  1990-06       Impact factor: 4.792

3.  Identification of lactococci and enterococci by colony hybridization with 23S rRNA-targeted oligonucleotide probes.

Authors:  D Betzl; W Ludwig; K H Schleifer
Journal:  Appl Environ Microbiol       Date:  1990-09       Impact factor: 4.792

Review 4.  New developments in the diagnosis of bloodstream infections.

Authors:  Remco P H Peters; Michiel A van Agtmael; Sven A Danner; Paul H M Savelkoul; Christina M J E Vandenbroucke-Grauls
Journal:  Lancet Infect Dis       Date:  2004-12       Impact factor: 25.071

5.  Direct identification of major blood culture pathogens, including Pseudomonas aeruginosa and Escherichia coli, by a panel of fluorescence in situ hybridization assays using peptide nucleic acid probes.

Authors:  M Søgaard; H Stender; H C Schønheyder
Journal:  J Clin Microbiol       Date:  2005-04       Impact factor: 5.948

6.  Faster identification of pathogens in positive blood cultures by fluorescence in situ hybridization in routine practice.

Authors:  Remco P H Peters; Paul H M Savelkoul; Alberdina M Simoons-Smit; Sven A Danner; Christina M J E Vandenbroucke-Grauls; Michiel A van Agtmael
Journal:  J Clin Microbiol       Date:  2006-01       Impact factor: 5.948

7.  Application of 23S rDNA-targeted oligonucleotide probes specific for enterococci to water hygiene control.

Authors:  E Frahm; I Heiber; S Hoffmann; C Koob; H Meier; W Ludwig; R Amann; K H Schleifer; U Obst
Journal:  Syst Appl Microbiol       Date:  1998-08       Impact factor: 4.022

8.  Optimized Fast-FISH with alpha-satellite probes: acceleration by microwave activation.

Authors:  M Durm; F M Haar; M Hausmann; H Ludwig; C Cremer
Journal:  Braz J Med Biol Res       Date:  1997-01       Impact factor: 2.590

9.  Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga-flavobacter-bacteroides in the natural environment.

Authors:  W Manz; R Amann; W Ludwig; M Vancanneyt; K H Schleifer
Journal:  Microbiology (Reading)       Date:  1996-05       Impact factor: 2.777

10.  FISHing in the microwave: the easy way to preserve proteins. I. Colocalization of DNA probes and surface antigens in human leukocytes.

Authors:  J Gosálvez; J De La Torre; M Pita; A Martinez-Ramirez; C López-Fernandez; V Goyanes; J López-Fernández
Journal:  Chromosome Res       Date:  2002       Impact factor: 4.620

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  15 in total

1.  Rapid identification of clinically relevant Enterococcus species by fluorescence in situ hybridization.

Authors:  Nele Wellinghausen; Melanie Bartel; Andreas Essig; Sven Poppert
Journal:  J Clin Microbiol       Date:  2007-08-01       Impact factor: 5.948

2.  Detection of mutations conferring resistance to linezolid in Enterococcus spp. by fluorescence in situ hybridization.

Authors:  Guido Werner; Melanie Bartel; Nele Wellinghausen; Andreas Essig; Ingo Klare; Wolfgang Witte; Sven Poppert
Journal:  J Clin Microbiol       Date:  2007-05-02       Impact factor: 5.948

3.  Evaluation of the LightCycler SeptiFast test in the rapid etiologic diagnostic of infectious endocarditis.

Authors:  J P Casalta; F Gouriet; V Roux; F Thuny; G Habib; D Raoult
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2008-12-02       Impact factor: 3.267

4.  Diagnostic performance and therapeutic impact of LightCycler SeptiFast assay in patients with suspected sepsis.

Authors:  V Herne; A Nelovkov; M Kütt; M Ivanova
Journal:  Eur J Microbiol Immunol (Bp)       Date:  2013-03-13

5.  Optimization of a two-step permeabilization fluorescence in situ hybridization (FISH) assay for the detection of Staphylococcus aureus.

Authors:  Thomas S Lawson; Russell E Connally; Subramanyam Vemulpad; James A Piper
Journal:  J Clin Lab Anal       Date:  2011       Impact factor: 2.352

Review 6.  Developments for improved diagnosis of bacterial bloodstream infections.

Authors:  A J M Loonen; P F G Wolffs; C A Bruggeman; A J C van den Brule
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2014-05-22       Impact factor: 3.267

Review 7.  Molecular methods and platforms for infectious diseases testing a review of FDA-approved and cleared assays.

Authors:  Rajyasree Emmadi; Jerry B Boonyaratanakornkit; Rangaraj Selvarangan; Venkatakrishna Shyamala; Barbara L Zimmer; Laurina Williams; Bonita Bryant; Ted Schutzbank; Michele M Schoonmaker; Jean A Amos Wilson; Leslie Hall; Preeti Pancholi; Kathryn Bernard
Journal:  J Mol Diagn       Date:  2011-08-25       Impact factor: 5.568

8.  Detection of Staphylococcus aureus with a fluorescence in situ hybridization that does not require lysostaphin.

Authors:  Thomas S Lawson; Russell E Connally; Jonathan R Iredell; Subramanyam Vemulpad; James A Piper
Journal:  J Clin Lab Anal       Date:  2011       Impact factor: 2.352

9.  Rapid identification of Acinetobacter spp. by fluorescence in situ hybridization (FISH) from colony and blood culture material.

Authors:  H Frickmann; A Essig; R M Hagen; M Riecker; K Jerke; D Ellison; S Poppert
Journal:  Eur J Microbiol Immunol (Bp)       Date:  2011-12-23

10.  Rapid discrimination of Haemophilus influenzae, H. parainfluenzae, and H. haemolyticus by fluorescence in situ hybridization (FISH) and two matrix-assisted laser-desorption-ionization time-of-flight mass spectrometry (MALDI-TOF-MS) platforms.

Authors:  Hagen Frickmann; Martin Christner; Martina Donat; Anja Berger; Andreas Essig; Andreas Podbielski; Ralf Matthias Hagen; Sven Poppert
Journal:  PLoS One       Date:  2013-04-30       Impact factor: 3.240

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