Literature DB >> 9779610

Application of 23S rDNA-targeted oligonucleotide probes specific for enterococci to water hygiene control.

E Frahm1, I Heiber, S Hoffmann, C Koob, H Meier, W Ludwig, R Amann, K H Schleifer, U Obst.   

Abstract

Identification of enterococci species by hybridization with recently designed species-specific and group-specific 23S rDNA-targeted oligonucleotide probes was superior to results obtained with a common biochemical test panel. Considering these findings, a molecular biological procedure for the detection of enterococci in water samples was developed. A short enrichment is followed by an amplification step and a hybridization reaction in microtiter plate format. The detection limit is about 1 CFU/ml, and results are available within 26 h.

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Year:  1998        PMID： 9779610     DOI： 10.1016/S0723-2020(98)80055-6

Source DB:  PubMed          Journal:  Syst Appl Microbiol        ISSN： 0723-2020            Impact factor:   4.022


  10 in total

1.  Fluorescent In situ hybridization allows rapid identification of microorganisms in blood cultures.

Authors:  V A Kempf; K Trebesius; I B Autenrieth
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

2.  Microarray-based identification of bacteria in clinical samples by solid-phase PCR amplification of 23S ribosomal DNA sequences.

Authors:  Georg Mitterer; Martin Huber; Ernst Leidinger; Claudia Kirisits; Werner Lubitz; Manfred W Mueller; Wolfgang M Schmidt
Journal:  J Clin Microbiol       Date:  2004-03       Impact factor: 5.948

3.  Rapid identification of pathogens in blood cultures with a modified fluorescence in situ hybridization assay.

Authors:  Remco P H Peters; Michiel A van Agtmael; Alberdina M Simoons-Smit; Sven A Danner; Christina M J E Vandenbroucke-Grauls; Paul H M Savelkoul
Journal:  J Clin Microbiol       Date:  2006-11       Impact factor: 5.948

4.  Spatial organization and composition of the mucosal flora in patients with inflammatory bowel disease.

Authors:  Alexander Swidsinski; Jutta Weber; Vera Loening-Baucke; Laura P Hale; Herbert Lochs
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

5.  Spatial organisation of microbiota in quiescent adenoiditis and tonsillitis.

Authors:  A Swidsinski; O Göktas; C Bessler; V Loening-Baucke; L P Hale; H Andree; M Weizenegger; M Hölzl; H Scherer; H Lochs
Journal:  J Clin Pathol       Date:  2006-05-12       Impact factor: 3.411

6.  Diversity of domain V of 23S rRNA gene sequence in different Enterococcus species.

Authors:  S Tsiodras; H S Gold; E P Coakley; C Wennersten; R C Moellering; G M Eliopoulos
Journal:  J Clin Microbiol       Date:  2000-11       Impact factor: 5.948

7.  Spatial organization of bacterial flora in normal and inflamed intestine: a fluorescence in situ hybridization study in mice.

Authors:  Alexander Swidsinski; Vera Loening-Baucke; Herbert Lochs; Laura-P Hale
Journal:  World J Gastroenterol       Date:  2005-02-28       Impact factor: 5.742

8.  23S rRNA gene-based enterococci community signatures in Lake Pontchartrain, Louisiana, USA, following urban runoff inputs after Hurricane Katrina.

Authors:  Hee-Sung Bae; Aixin Hou
Journal:  Microb Ecol       Date:  2012-12-28       Impact factor: 4.552

9.  Microbial quality and direct PCR identification of lactic acid bacteria and nonpathogenic Staphylococci from artisanal low-acid sausages.

Authors:  T Aymerich; B Martín; M Garriga; M Hugas
Journal:  Appl Environ Microbiol       Date:  2003-08       Impact factor: 4.792

10.  Growth Forms of Gardnerella spp. and Lactobacillus spp. on Vaginal Cells.

Authors:  Hyunsul Jung; Marthie M Ehlers; Remco P H Peters; Hennie Lombaard; Mathys J Redelinghuys; Johanna E Bezuidenhoudt; Marleen M Kock
Journal:  Front Cell Infect Microbiol       Date:  2020-02-28       Impact factor: 5.293
  10 in total

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