Literature DB >> 11993934

FISHing in the microwave: the easy way to preserve proteins. I. Colocalization of DNA probes and surface antigens in human leukocytes.

J Gosálvez1, J De La Torre, M Pita, A Martinez-Ramirez, C López-Fernandez, V Goyanes, J López-Fernández.   

Abstract

Most of the techniques for simultaneous visualization of proteic cell components and DNA probes are difficult to carry out. We have developed an alternative protocol for simultaneous visualization of DNA probes and cell surface antigens in human cells. The method exploits microwave energy to denature DNA and to anneal the probe after antigen fluorescent detection of unfixed cells previously embedded in a microgel. CD3 antigens and whole genome DNA probes or specific repetitive DNA sequences were colocalized in peripheral blood samples. The results show a strong, specific and consistent hybridization pattern in each cell that allowed correlation between cell subtype (PROTEINS) and nuclear phenotype (DNA).

Entities:  

Mesh：

Substances：

Year:  2002        PMID： 11993934     DOI： 10.1023/a:1014901218648

Source DB:  PubMed          Journal:  Chromosome Res        ISSN： 0967-3849            Impact factor:   4.620


  7 in total

1.  Application of FISH for in situ detection and quantification of DNA breakage.

Authors:  J L Fernández; V J Goyanes; J Ramiro-Díaz; J Gosálvez
Journal:  Cytogenet Cell Genet       Date:  1998

2.  Optimized Fast-FISH with alpha-satellite probes: acceleration by microwave activation.

Authors:  M Durm; F M Haar; M Hausmann; H Ludwig; C Cremer
Journal:  Braz J Med Biol Res       Date:  1997-01       Impact factor: 2.590

3.  The application of microwave denaturation in comparative genomic hybridization.

Authors:  M de Meulemeester; A Vink; M Jakobs; M Hermsen; M Steenman; R Slater; A Dietrich; M Mannens
Journal:  Genet Anal       Date:  1996-11

4.  Polymorphisms for the size of heterochromatic regions allow sex-independent quantification of post-BMT chimerism targeting metaphase and interphase cells.

Authors:  I Buño; J L Díez-Martín; C López-Fernández; J L Fernández; J Gosálvez
Journal:  Haematologica       Date:  1999-02       Impact factor: 9.941

5.  Combined immunocytochemistry and fluorescence in situ hybridization for simultaneous tricolor detection of cell cycle, genomic, and phenotypic parameters of tumor cells.

Authors:  E J Speel; J Herbergs; F C Ramaekers; A H Hopman
Journal:  J Histochem Cytochem       Date:  1994-07       Impact factor: 2.479

6.  A method for simultaneous study of the karyotype, morphology, and immunologic phenotype of mitotic cells in hematologic malignancies.

Authors:  L Teerenhovi; S Knuutila; M Ekblom; L Rossi; G H Borgström; J K Tallman; L Andersson; A de la Chapelle
Journal:  Blood       Date:  1984-11       Impact factor: 22.113

7.  Detection of mixed chimerism and leukemic relapse after allogeneic bone marrow transplantation in subpopulations of leucocytes by fluorescent in situ hybridization in combination with the simultaneous immunophenotypic analysis of interphase cells.

Authors:  G Kögler; H H Wolf; A Heyll; G Arkesteijn; P Wernet
Journal:  Bone Marrow Transplant       Date:  1995-01       Impact factor: 5.483
  7 in total
  1 in total

1.  Rapid identification of pathogens in blood cultures with a modified fluorescence in situ hybridization assay.

Authors:  Remco P H Peters; Michiel A van Agtmael; Alberdina M Simoons-Smit; Sven A Danner; Christina M J E Vandenbroucke-Grauls; Paul H M Savelkoul
Journal:  J Clin Microbiol       Date:  2006-11       Impact factor: 5.948
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.