Literature DB >> 2275539

Identification of lactococci and enterococci by colony hybridization with 23S rRNA-targeted oligonucleotide probes.

D Betzl1, W Ludwig, K H Schleifer.   

Abstract

Specific sequences of 23S rRNA of Lactococcus lactis, Enterococcus faecalis, Enteroccus faecium, and Enterococcus malodoratus/Enterococcus avium were identified, and complementary oligonucleotide probes were synthesized. The specificity of the probes was evaluated by dot blot and colony hybridizations. The probes can be used for the specific detection and identification of colonies of the corresponding species in mixed cultures.

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Year:  1990        PMID： 2275539      PMCID： PMC184868          DOI： 10.1128/aem.56.9.2927-2929.1990

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  11 in total

1.  Detection of hemolytic Listeria monocytogenes by using DNA colony hybridization.

Authors:  A R Datta; B A Wentz; W E Hill
Journal:  Appl Environ Microbiol       Date:  1987-09       Impact factor: 4.792

2.  DNA hybridization probe for the Pseudomonas fluorescens group.

Authors:  H Festl; W Ludwig; K H Schleifer
Journal:  Appl Environ Microbiol       Date:  1986-11       Impact factor: 4.792

3.  Reverse transcriptase sequencing of 16S ribosomal RNA from Faenia rectivirgula, Pseudonocardia thermophila and Saccharopolyspora hirsuta, three wall type IV actinomycetes which lack mycolic acids.

Authors:  T M Embley; J Smida; E Stackebrandt
Journal:  J Gen Microbiol       Date:  1988-04

4.  Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells.

Authors:  S J Giovannoni; E F DeLong; G J Olsen; N R Pace
Journal:  J Bacteriol       Date:  1988-02       Impact factor: 3.490

5.  DNA probes with different specificities from a cloned 23S rRNA gene of Micrococcus luteus.

Authors:  A Regensburger; W Ludwig; K H Schleifer
Journal:  J Gen Microbiol       Date:  1988-05

Review 6.  Analytical strategies for the use of DNA probes.

Authors:  J A Matthews; L J Kricka
Journal:  Anal Biochem       Date:  1988-02-15       Impact factor: 3.365

7.  A rapid procedure for colony screening using nylon filters.

Authors:  L Buluwela; A Forster; T Boehm; T H Rabbitts
Journal:  Nucleic Acids Res       Date:  1989-01-11       Impact factor: 16.971

8.  A membrane-filter technique for the detection of complementary DNA.

Authors:  D T Denhardt
Journal:  Biochem Biophys Res Commun       Date:  1966-06-13       Impact factor: 3.575

9.  The 23S ribosomal RNA higher-order structure of Pseudomonas cepacia and other prokaryotes.

Authors:  P Höpfl; W Ludwig; K H Schleifer; N Larsen
Journal:  Eur J Biochem       Date:  1989-11-06

10.  Improved medium for lactic streptococci and their bacteriophages.

Authors:  B E Terzaghi; W E Sandine
Journal:  Appl Microbiol       Date:  1975-06
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  24 in total

1.  Development of a PCR assay for rapid detection of enterococci.

Authors:  D Ke; F J Picard; F Martineau; C Ménard; P H Roy; M Ouellette; M G Bergeron
Journal:  J Clin Microbiol       Date:  1999-11       Impact factor: 5.948

2.  Identification of Enterococcus spp. with a biochemical key.

Authors:  A Manero; A R Blanch
Journal:  Appl Environ Microbiol       Date:  1999-10       Impact factor: 4.792

3.  Whole-cell versus total RNA extraction for analysis of microbial community structure with 16S rRNA-targeted oligonucleotide probes in salt marsh sediments.

Authors:  M E Frischer; J M Danforth; M A Newton Healy; F M Saunders
Journal:  Appl Environ Microbiol       Date:  2000-07       Impact factor: 4.792

4.  Improved enumeration of lactic acid bacteria in mesophilic dairy starter cultures by using multiplex quantitative real-time PCR and flow cytometry-fluorescence in situ hybridization.

Authors:  Udo Friedrich; Jan Lenke
Journal:  Appl Environ Microbiol       Date:  2006-06       Impact factor: 4.792

5.  Rapid genomic fingerprinting of Lactococcus lactis strains by arbitrarily primed polymerase chain reaction with 32P and fluorescent labels.

Authors:  M R Cancilla; I B Powell; A J Hillier; B E Davidson
Journal:  Appl Environ Microbiol       Date:  1992-05       Impact factor: 4.792

Review 6.  [Gene probes and their use in microbiology].

Authors:  K H Schleifer; W Ludwig; R Amann
Journal:  Naturwissenschaften       Date:  1992-05

7.  Identification of mesophilic lactic acid bacteria by using polymerase chain reaction-amplified variable regions of 16S rRNA and specific DNA probes.

Authors:  N Klijn; A H Weerkamp; W M de Vos
Journal:  Appl Environ Microbiol       Date:  1991-11       Impact factor: 4.792

8.  Rapid identification of pathogens in blood cultures with a modified fluorescence in situ hybridization assay.

Authors:  Remco P H Peters; Michiel A van Agtmael; Alberdina M Simoons-Smit; Sven A Danner; Christina M J E Vandenbroucke-Grauls; Paul H M Savelkoul
Journal:  J Clin Microbiol       Date:  2006-11       Impact factor: 5.948

Review 9.  Phylogenetic identification and in situ detection of individual microbial cells without cultivation.

Authors:  R I Amann; W Ludwig; K H Schleifer
Journal:  Microbiol Rev       Date:  1995-03

10.  Generation of auxotrophic mutants of Enterococcus faecalis.

Authors:  X Li; G M Weinstock; B E Murray
Journal:  J Bacteriol       Date:  1995-12       Impact factor: 3.490
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