Literature DB >> 16954281

Comparison of API 20E and invA PCR for identification of Salmonella enterica isolates from swine production units.

Daniele M Nucera1, Carol W Maddox, Patricia Hoien-Dalen, Ronald M Weigel.   

Abstract

API 20E and invA PCR were evaluated for the identification of Salmonella enterica isolates from swine farms. API 20E had the highest agreement with other tests at the 99.9% likelihood level. Both tests had 100% sensitivity and 96% specificity compared to 16S rRNA sequencing. Compared to serotyping, both tests had 96% sensitivity; specificity was 86% for API 20E and 79% for invA PCR.

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Year:  2006        PMID： 16954281      PMCID： PMC1594722          DOI： 10.1128/JCM.00972-06

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

1.  Multicenter validation of the analytical accuracy of Salmonella PCR: towards an international standard.

Authors:  Burkhard Malorny; Jeffrey Hoorfar; Cornelia Bunge; Reiner Helmuth
Journal:  Appl Environ Microbiol       Date:  2003-01       Impact factor: 4.792

2.  Use of pooled samples for the detection of Salmonella in feces by polymerase chain reaction.

Authors:  Randall S Singer; Cara L Cooke; Carol W Maddox; Richard E Isaacson; Richard L Wallace
Journal:  J Vet Diagn Invest       Date:  2006-07       Impact factor: 1.279

3.  Correlation studies of entero-set 20, API 20E and conventional media systems for Enterobacteriaceae identification.

Authors:  K E Aldridge; R L Hodges
Journal:  J Clin Microbiol       Date:  1981-01       Impact factor: 5.948

4.  Comparison of the automicrobic system with API, enterotube, micro-ID, micro-media systems, and conventional methods for identification of Enterobacteriaceae.

Authors:  M T Kelly; J M Latimer
Journal:  J Clin Microbiol       Date:  1980-11       Impact factor: 5.948

5.  Comparison of Enteric-Tek with API 20E and conventional methods for identification of Enterobacteriaceae.

Authors:  D A Bruckner; V Clark; W J Martin
Journal:  J Clin Microbiol       Date:  1982-01       Impact factor: 5.948

6.  Automated 5' nuclease PCR assay for identification of Salmonella enterica.

Authors:  J Hoorfar; P Ahrens; P Rådström
Journal:  J Clin Microbiol       Date:  2000-09       Impact factor: 5.948

7.  PCR amplification of the Salmonella typhimurium fimY gene sequence to detect the Salmonella species.

Authors:  K S Yeh; T H Chen; C W Liao; C S Chang; H C Lo
Journal:  Int J Food Microbiol       Date:  2002-10-25       Impact factor: 5.277

8.  Comparison of micro-ID, API 20E, and conventional media systems in identification of Enterobacteriaceae.

Authors:  K E Aldridge; B B Gardner; S J Clark; J M Matsen
Journal:  J Clin Microbiol       Date:  1978-06       Impact factor: 5.948

9.  Detection and identification of salmonellas from poultry-related samples by PCR.

Authors:  S D Oliveira; L R Santos; D M T Schuch; A B Silva; C T P Salle; C W Canal
Journal:  Vet Microbiol       Date:  2002-06-05       Impact factor: 3.293

10.  Use of the API 20E system to identify veterinary Enterobacteriaceae.

Authors:  E C Swanson; M T Collins
Journal:  J Clin Microbiol       Date:  1980-07       Impact factor: 5.948
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  13 in total

1.  Occurrence of Antimicrobial-Resistant Escherichia coli and Salmonella enterica in the Beef Cattle Production and Processing Continuum.

Authors:  John W Schmidt; Getahun E Agga; Joseph M Bosilevac; Dayna M Brichta-Harhay; Steven D Shackelford; Rong Wang; Tommy L Wheeler; Terrance M Arthur
Journal:  Appl Environ Microbiol       Date:  2014-11-14       Impact factor: 4.792

2.  Diversity of multidrug-resistant salmonella enterica strains associated with cattle at harvest in the United States.

Authors:  Dayna M Brichta-Harhay; Terrance M Arthur; Joseph M Bosilevac; Norasak Kalchayanand; Steven D Shackelford; Tommy L Wheeler; Mohammad Koohmaraie
Journal:  Appl Environ Microbiol       Date:  2011-01-14       Impact factor: 4.792

3.  Prevalence, enumeration, serotypes, and antimicrobial resistance phenotypes of salmonella enterica isolates from carcasses at two large United States pork processing plants.

Authors:  John W Schmidt; Dayna M Brichta-Harhay; Norasak Kalchayanand; Joseph M Bosilevac; Steven D Shackelford; Tommy L Wheeler; Mohammad Koohmaraie
Journal:  Appl Environ Microbiol       Date:  2012-02-10       Impact factor: 4.792

4.  Diagnostic accuracy of class 1 integron PCR method in detection of antibiotic resistance in Salmonella isolates from swine production systems.

Authors:  Sangeeta Rao; Carol W Maddox; Patricia Hoien-Dalen; Sara Lanka; Ronald M Weigel
Journal:  J Clin Microbiol       Date:  2008-01-03       Impact factor: 5.948

5.  Prevalence and characterization of salmonellae in commercial ground beef in the United States.

Authors:  Joseph M Bosilevac; Michael N Guerini; Norasak Kalchayanand; Mohammad Koohmaraie
Journal:  Appl Environ Microbiol       Date:  2009-02-06       Impact factor: 4.792

6.  Cross-sectional study examining Salmonella enterica carriage in subiliac lymph nodes of cull and feedlot cattle at harvest.

Authors:  Sara E Gragg; Guy H Loneragan; Mindy M Brashears; Terrance M Arthur; Joseph M Bosilevac; Norasak Kalchayanand; Rong Wang; John W Schmidt; J Chance Brooks; Steven D Shackelford; Tommy L Wheeler; Tyson R Brown; Thomas S Edrington; Dayna M Brichta-Harhay
Journal:  Foodborne Pathog Dis       Date:  2013-04       Impact factor: 3.171

7.  Salmonella and Escherichia coli O157:H7 contamination on hides and carcasses of cull cattle presented for slaughter in the United States: an evaluation of prevalence and bacterial loads by immunomagnetic separation and direct plating methods.

Authors:  Dayna M Brichta-Harhay; Michael N Guerini; Terrance M Arthur; Joseph M Bosilevac; Norasak Kalchayanand; Steven D Shackelford; Tommy L Wheeler; Mohammad Koohmaraie
Journal:  Appl Environ Microbiol       Date:  2008-08-22       Impact factor: 4.792

8.  Antimicrobial-Resistant Bacterial Populations and Antimicrobial Resistance Genes Obtained from Environments Impacted by Livestock and Municipal Waste.

Authors:  Getahun E Agga; Terrance M Arthur; Lisa M Durso; Dayna M Harhay; John W Schmidt
Journal:  PLoS One       Date:  2015-07-21       Impact factor: 3.240

9.  Outer Membrane Protein C (ompC) Gene as the Target for Diagnosis of Salmonella Species Isolated from Human and Animal Sources.

Authors:  Alaa Abdel-Kadhim Jawad; Alaa H Al-Charrakh
Journal:  Avicenna J Med Biotechnol       Date:  2016 Jan-Mar

10.  Molecular beacon-based real-time PCR detection of primary isolates of Salmonella Typhimurium and Salmonella Enteritidis in environmental and clinical samples.

Authors:  Andreas V Hadjinicolaou; Victoria L Demetriou; Maria A Emmanuel; Charalambos K Kakoyiannis; Leondios G Kostrikis
Journal:  BMC Microbiol       Date:  2009-05-19       Impact factor: 3.605
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