Literature DB >> 16744552

Appendix: Analysis of pH-dependent kinetics in up to four reactive hydronic states.

S M Brocklehurst1, K Brocklehurst.   

Abstract

Year:  1988        PMID： 16744552      PMCID： PMC1135445          DOI： 10.1042/bj2560556

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


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  9 in total

1.  PH-dependence of the steady-state rate of a two-step enzymic reaction.

Authors:  K Brocklehurst; H B Dixon
Journal:  Biochem J       Date:  1976-04-01       Impact factor: 3.857

2.  pH-activity curves for enzyme-catalysed reactions in which the hydron is a product or reactant.

Authors:  H B Dixon; K Brocklehurst; K F Tipton
Journal:  Biochem J       Date:  1987-12-01       Impact factor: 3.857

3.  The pH-dependence of second-order rate constants of enzyme modification may provide free-reactant pKa values.

Authors:  K Brocklehurst; H B Dixon
Journal:  Biochem J       Date:  1977-12-01       Impact factor: 3.857

4.  Characterization of the papain active centre by using two-protonic-state electrophiles as reactivity probes. Evidence for nucleophilic reactivity in the un-interrupted cysteine-25-histidine-159 interactive system.

Authors:  M Shipton; K Brochlehurst
Journal:  Biochem J       Date:  1978-05-01       Impact factor: 3.857

5.  The equilibrium assumption is valid for the kinetic treatment of most time-dependent protein-modification reactions.

Authors:  K Brocklehurst
Journal:  Biochem J       Date:  1979-09-01       Impact factor: 3.857

6.  Supracrystallographic resolution of interactions contributing to enzyme catalysis by use of natural structural variants and reactivity-probe kinetics.

Authors:  K Brocklehurst; S M Brocklehurst; D Kowlessur; M O'Driscoll; G Patel; E Salih; W Templeton; E Thomas; C M Topham; F Willenbrock
Journal:  Biochem J       Date:  1988-12-01       Impact factor: 3.857

7.  Effects of pH on enzymes.

Authors:  K F Tipton; H B Dixon
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

8.  Preparation of fully active ficin from Ficus glabrata by covalent chromatography and characterization of its active centre by using 2,2'-depyridyl disulphide as a reactivity probe.

Authors:  J P Malthouse; K Brocklehurst
Journal:  Biochem J       Date:  1976-11       Impact factor: 3.857

9.  Consequences of molecular recognition in the S1-S2 intersubsite region of papain for catalytic-site chemistry. Change in pH-dependence characteristics and generation of an inverse solvent kinetic isotope effect by introduction of a P1-P2 amide bond into a two-protonic-state reactivity probe.

Authors:  K Brocklehurst; D Kowlessur; G Patel; W Templeton; K Quigley; E W Thomas; C W Wharton; F Willenbrock; R J Szawelski
Journal:  Biochem J       Date:  1988-03-15       Impact factor: 3.857
  9 in total
  8 in total

1.  The interplay of electrostatic fields and binding interactions determining catalytic-site reactivity in actinidin. A possible origin of differences in the behaviour of actinidin and papain.

Authors:  D Kowlessur; M O'Driscoll; C M Topham; W Templeton; E W Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1989-04-15       Impact factor: 3.857

2.  Identification of signalling and non-signalling binding contributions to enzyme reactivity. Alternative combinations of binding interactions provide for change in transition-state geometry in reactions of papain.

Authors:  D Kowlessur; C M Topham; E W Thomas; M O'Driscoll; W Templeton; K Brocklehurst
Journal:  Biochem J       Date:  1989-03-15       Impact factor: 3.857

3.  Supracrystallographic resolution of interactions contributing to enzyme catalysis by use of natural structural variants and reactivity-probe kinetics.

Authors:  K Brocklehurst; S M Brocklehurst; D Kowlessur; M O'Driscoll; G Patel; E Salih; W Templeton; E Thomas; C M Topham; F Willenbrock
Journal:  Biochem J       Date:  1988-12-01       Impact factor: 3.857

4.  Clarification of the pH-dependent kinetic behaviour of papain by using reactivity probes and analysis of alkylation and catalysed acylation reactions in terms of multihydronic state models: implications for electrostatics calculations and interpretation of the consequences of site-specific mutations such as Asp-158-Asn and Asp-158-Glu.

Authors:  G W Mellor; M Patel; E W Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1993-08-15       Impact factor: 3.857

5.  A polyclonal antibody preparation with Michaelian catalytic properties.

Authors:  G Gallacher; C S Jackson; M Searcey; G T Badman; R Goel; C M Topham; G W Mellor; K Brocklehurst
Journal:  Biochem J       Date:  1991-11-01       Impact factor: 3.857

6.  Catalytic-site characteristics of the porcine calpain II 80 kDa/18 kDa heterodimer revealed by selective reaction of its essential thiol group with two-hydronic-state time-dependent inhibitors: evidence for a catalytic site Cys/His interactive system and an ionizing modulatory group.

Authors:  G W Mellor; S K Sreedharan; D Kowlessur; E W Thomas; K Brocklehurst
Journal:  Biochem J       Date:  1993-02-15       Impact factor: 3.857

7.  A re-appraisal of the structural basis of stereochemical recognition in papain. Insensitivity of binding-site-catalytic-site signalling to P2-chirality in a time-dependent inhibition.

Authors:  W Templeton; D Kowlessur; E W Thomas; C M Topham; K Brocklehurst
Journal:  Biochem J       Date:  1990-03-15       Impact factor: 3.857

8.  Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.

Authors:  M P Thomas; C M Topham; D Kowlessur; G W Mellor; E W Thomas; D Whitford; K Brocklehurst
Journal:  Biochem J       Date:  1994-06-15       Impact factor: 3.857
  8 in total

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