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Literature DB >> 16208011

Evaluation of universal probes and primer sets for assessing total bacterial load in clinical samples: general implications and practical use in endodontic antimicrobial therapy.

H P Horz¹, M E Vianna, B P F A Gomes, G Conrads.

Abstract

By re-examining 10 previously published "universal" PCR assays using the ARB phylogenetic software package and database with 41,000 16S rRNA gene sequences, we found that they differed considerably in their coverage of the domain Bacteria. We evaluated the broadest-range real-time quantitative PCR protocol for its efficacy in measuring the antimicrobial effects of endodontic treatments.

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Year: 2005 PMID： 16208011 PMCID： PMC1248440 DOI： 10.1128/JCM.43.10.5332-5337.2005

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

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