Literature DB >> 17596351

Use of quantitative PCR and culture methods to characterize ecological flux in bacterial biofilms.

F Dalwai1, D A Spratt, J Pratten.   

Abstract

An in vitro model of supragingival plaque associated with gingivitis was characterized by traditional culture techniques, comparative 16S rRNA gene sequencing of isolates, and quantitative PCR (QPCR). Actinomyces naeslundii, Prevotella spp., and Porphyromonas gingivalis increased under conditions emulating gingivitis. Gram-negative species and total bacteria were dramatically underestimated by culture compared to the estimates obtained by QPCR.

Entities:  

Mesh：

Substances：

Year:  2007        PMID： 17596351      PMCID： PMC2045240          DOI： 10.1128/JCM.01131-07

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  31 in total

1.  Quantitative real-time PCR for Porphyromonas gingivalis and total bacteria.

Authors:  S R Lyons; A L Griffen; E J Leys
Journal:  J Clin Microbiol       Date:  2000-06       Impact factor: 5.948

2.  Evaluation of universal probes and primer sets for assessing total bacterial load in clinical samples: general implications and practical use in endodontic antimicrobial therapy.

Authors:  H P Horz; M E Vianna; B P F A Gomes; G Conrads
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

3.  Streptococcal diversity in oral biofilms with respect to salivary function.

Authors:  J D Rudney; Y Pan; R Chen
Journal:  Arch Oral Biol       Date:  2003-07       Impact factor: 2.633

4.  Comparison of subgingival bacterial sampling with oral lavage for detection and quantification of periodontal pathogens by real-time polymerase chain reaction.

Authors:  Khalil Boutaga; Paul H M Savelkoul; Edwin G Winkel; Arie J van Winkelhoff
Journal:  J Periodontol       Date:  2007-01       Impact factor: 6.993

5.  Modeling shifts in microbial populations associated with health or disease.

Authors:  F Dalwai; D A Spratt; J Pratten
Journal:  Appl Environ Microbiol       Date:  2006-05       Impact factor: 4.792

6.  Quantitative detection of periodontopathogens by real-time PCR.

Authors:  Claudia Nonnenmacher; Alexander Dalpke; Reinier Mutters; Klaus Heeg
Journal:  J Microbiol Methods       Date:  2004-10       Impact factor: 2.363

7.  Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, tetQ gene and total bacteria.

Authors:  Hiroshi Maeda; Chiyo Fujimoto; Yasuhiro Haruki; Takemasa Maeda; Susumu Kokeguchi; Millan Petelin; Hideo Arai; Ichiro Tanimoto; Fusanori Nishimura; Shogo Takashiba
Journal:  FEMS Immunol Med Microbiol       Date:  2003-10-24

8.  Development of a selective medium for detection and enumeration of Actinomyces viscosus and Actinomyces naeslundii in dental plaque.

Authors:  L J Zylber; H V Jordan
Journal:  J Clin Microbiol       Date:  1982-02       Impact factor: 5.948

9.  Bacteriology of experimental gingivitis in young adult humans.

Authors:  W E Moore; L V Holdeman; R M Smibert; I J Good; J A Burmeister; K G Palcanis; R R Ranney
Journal:  Infect Immun       Date:  1982-11       Impact factor: 3.441

10.  Bacteriology of human experimental gingivitis: effect of plaque age.

Authors:  S A Syed; W J Loesche
Journal:  Infect Immun       Date:  1978-09       Impact factor: 3.441
View more
  11 in total

1.  Effects of statins on multispecies oral biofilm identify simvastatin as a drug candidate targeting Porphyromonas gingivalis.

Authors:  Marta Kamińska; Ardita Aliko; Annelie Hellvard; Ewa Bielecka; Veronika Binder; Agata Marczyk; Jan Potempa; Nicolas Delaleu; Tomasz Kantyka; Piotr Mydel
Journal:  J Periodontol       Date:  2018-12-26       Impact factor: 6.993

2.  Possible variation of the human oral bacterial community after wearing removable partial dentures by DGGE.

Authors:  Xiao Zhu; Shaohai Wang; Yihai Gu; Xiaoyu Li; Hui Yan; He Yan; Shin-Ichi Miyoshi; Lei Shi
Journal:  World J Microbiol Biotechnol       Date:  2012-03-06       Impact factor: 3.312

3.  Loss of NHE3 alters gut microbiota composition and influences Bacteroides thetaiotaomicron growth.

Authors:  Melinda A Engevik; Eitaro Aihara; Marshall H Montrose; Gary E Shull; Daniel J Hassett; Roger T Worrell
Journal:  Am J Physiol Gastrointest Liver Physiol       Date:  2013-09-26       Impact factor: 4.052

4.  Molecular detection of bacteria associated to caries activity in dentinal lesions.

Authors:  Beatriz Gonçalves Neves; Rafael Nóbrega Stipp; Daniela da Silva Bezerra; Sarah Florindo de Figueiredo Guedes; Lidiany Karla Azevedo Rodrigues
Journal:  Clin Oral Investig       Date:  2016-11-12       Impact factor: 3.573

5.  Actinomyces in chronic granulomatous disease: an emerging and unanticipated pathogen.

Authors:  Janine Reichenbach; Uri Lopatin; Nizar Mahlaoui; Bojana Beovic; Ulrich Siler; Reinhard Zbinden; Reinhard A Seger; Louise Galmiche; Nicole Brousse; Samer Kayal; Tayfun Güngör; Stéphane Blanche; Steven M Holland
Journal:  Clin Infect Dis       Date:  2009-12-01       Impact factor: 9.079

6.  Microbial profiling of dental plaque from mechanically ventilated patients.

Authors:  Kirsty M Sands; Joshua A Twigg; Michael A O Lewis; Matt P Wise; Julian R Marchesi; Ann Smith; Melanie J Wilson; David W Williams
Journal:  J Med Microbiol       Date:  2015-12-21       Impact factor: 2.472

7.  Rapid Multiplex Real-Time PCR Method for the Detection and Quantification of Selected Cariogenic and Periodontal Bacteria.

Authors:  Jan Lochman; Martina Zapletalova; Hana Poskerova; Lydie Izakovicova Holla; Petra Borilova Linhartova
Journal:  Diagnostics (Basel)       Date:  2019-12-22

8.  MiR-21 Is Remotely Governed by the Commensal Bacteria and Impairs Anti-TB Immunity by Down-Regulating IFN-γ.

Authors:  Fang Yang; Yi Yang; Yiwei Chen; Guobao Li; Guoliang Zhang; Lingming Chen; Zhiyi Zhang; Qiongdan Mai; Gucheng Zeng
Journal:  Front Microbiol       Date:  2021-01-21       Impact factor: 5.640

9.  As-yet-uncultivated oral bacteria: breadth and association with oral and extra-oral diseases.

Authors:  José F Siqueira; Isabela N Rôças
Journal:  J Oral Microbiol       Date:  2013-05-23       Impact factor: 5.474

10.  Chair-side quantitative oral-microflora screening for assessing familial correlation of periodontal status and caries prevalence.

Authors:  Yung-Kai Huang; Wei-Fang Lee; Meng-Jiy Wang; Yus-Han Sophie Chang; Wen-Shiun Tchaou; Wei-Jen Chang; Sheng-Yang Lee; Joen-Rong Sheu; Nai-Chia Teng
Journal:  PLoS One       Date:  2014-01-30       Impact factor: 3.240
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.