| Literature DB >> 9451814 |
F von Wintzingerode1, U B Göbel, E Stackebrandt.
Abstract
After nearly 10 years of PCR-based analysis of prokaryotic small-subunit ribosomal RNAs for ecological studies it seems necessary to summarize reported pitfalls of this approach which will most likely lead to an erroneous description on the microbial diversity of a given habitat. The following article will cover specific aspects of sample collection, cell lysis, nucleic acid extraction, PCR amplification, separation of amplified DNA, application of nucleic probes and data analysis.Entities:
Mesh：
Substances：
Year: 1997 PMID： 9451814 DOI： 10.1111/j.1574-6976.1997.tb00351.x
Source DB: PubMed Journal: FEMS Microbiol Rev ISSN： 0168-6445 Impact factor: 16.408