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Literature DB >> 15459666

Imaging gene expression in single living cells.

Yaron Shav-Tal¹, Robert H Singer, Xavier Darzacq.

Abstract

Technical advances in the field of live-cell imaging have introduced the cell biologist to a new, dynamic, subcellular world. The static world of molecules in fixed cells has now been extended to the time dimension. This allows the visualization and quantification of gene expression and intracellular trafficking events of the studied molecules and the associated enzymatic processes in individual cells, in real time.

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Year: 2004 PMID： 15459666 PMCID： PMC4942131 DOI： 10.1038/nrm1494

Source DB: PubMed Journal: Nat Rev Mol Cell Biol ISSN： 1471-0072 Impact factor: 94.444

68 in total

1. The glucocorticoid receptor: rapid exchange with regulatory sites in living cells.

Authors: J G McNally; W G Müller; D Walker; R Wolford; G L Hager
Journal: Science Date: 2000-02-18 Impact factor: 47.728

Review 2. Kinetic modelling approaches to in vivo imaging.

Authors: R D Phair; T Misteli
Journal: Nat Rev Mol Cell Biol Date: 2001-12 Impact factor: 94.444

Review 3. Gene expression and the myth of the average cell.

Authors: Jeffrey M Levsky; Robert H Singer
Journal: Trends Cell Biol Date: 2003-01 Impact factor: 20.808

4. Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking.

Authors: Maxime Dahan; Sabine Lévi; Camilla Luccardini; Philippe Rostaing; Béatrice Riveau; Antoine Triller
Journal: Science Date: 2003-10-17 Impact factor: 47.728

Review 5. 4D imaging to assay complex dynamics in live specimens.

Authors: Daniel Gerlich; Jan Ellenberg
Journal: Nat Cell Biol Date: 2003-09 Impact factor: 28.824

6. Stochastic patterns in globin gene expression are established prior to transcriptional activation and are clonally inherited.

Authors: Mariken de Krom; Mariette van de Corput; Marieke von Lindern; Frank Grosveld; John Strouboulis
Journal: Mol Cell Date: 2002-06 Impact factor: 17.970

7. Global chromosome positions are transmitted through mitosis in mammalian cells.

Authors: Daniel Gerlich; Joël Beaudouin; Bernd Kalbfuss; Nathalie Daigle; Roland Eils; Jan Ellenberg
Journal: Cell Date: 2003-03-21 Impact factor: 41.582

Review 8. Single-molecule visualization in cell biology.

Authors: Yasushi Sako; Toshio Yanagida
Journal: Nat Rev Mol Cell Biol Date: 2003-09 Impact factor: 94.444

9. Fluorescent protein spectra.

Authors: G Patterson; R N Day; D Piston
Journal: J Cell Sci Date: 2001-03 Impact factor: 5.285

10. In vivo analyses of cytoplasmic transport and cytoskeletal organization during Drosophila oogenesis: characterization of a multi-step anterior localization pathway.

Authors: W E Theurkauf; T I Hazelrigg
Journal: Development Date: 1998-09 Impact factor: 6.868

40 in total

Review 1. Proteins on the move: insights gained from fluorescent protein technologies.

Authors: Atsushi Miyawaki
Journal: Nat Rev Mol Cell Biol Date: 2011-09-23 Impact factor: 94.444

Review 2. The moving parts of the nucleolus.

Authors: M O J Olson; Miroslav Dundr
Journal: Histochem Cell Biol Date: 2005-03-02 Impact factor: 4.304

Imaging gene expression in single living cells.

1. The glucocorticoid receptor: rapid exchange with regulatory sites in living cells.

Review 2. Kinetic modelling approaches to in vivo imaging.

Review 3. Gene expression and the myth of the average cell.

4. Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking.

Review 5. 4D imaging to assay complex dynamics in live specimens.

6. Stochastic patterns in globin gene expression are established prior to transcriptional activation and are clonally inherited.

7. Global chromosome positions are transmitted through mitosis in mammalian cells.

Review 8. Single-molecule visualization in cell biology.

9. Fluorescent protein spectra.

10. In vivo analyses of cytoplasmic transport and cytoskeletal organization during Drosophila oogenesis: characterization of a multi-step anterior localization pathway.

Review 1. Proteins on the move: insights gained from fluorescent protein technologies.

Review 2. The moving parts of the nucleolus.

3. From analog to digital: exploring cell dynamics with single quantum dots.

Review 4. Dynamics of transcription and mRNA export.

Review 5. The Cajal body: a meeting place for spliceosomal snRNPs in the nuclear maze.

6. Classical versus stochastic kinetics modeling of biochemical reaction systems.

7. A reaction-diffusion model to study RNA motion by quantitative fluorescence recovery after photobleaching.

8. In vivo dynamics of RNA polymerase II transcription.

9. RNA-based affinity purification reveals 7SK RNPs with distinct composition and regulation.

10. Single-cell c-myc gene expression in relationship to nuclear domains.