AIMS: The recommended method for the international sensitivity index (ISI) calibration of whole blood point of care testing (POCT) prothrombin time (PT) systems was originally described by Tripodi et al in 1993 but is too complex and demanding. The present European Concerted Action on Anticoagulation (ECAA) study aimed to assess the reliability of simpler ISI calibration using lyophilised plasma samples. METHODS: ISI calibrations using three different types of ECAA lyophilised plasma samples (artificially depleted, individual, and pooled coumarin) were compared with whole blood calibrations on CoaguChek Mini and TAS PT-NC POCT monitors at 10 centres. RESULTS: With CoaguChek Mini systems, lyophilised coumarin plasma samples (both single donation and pooled) gave ISI and international normalised ratio (INR) values comparable to whole blood. With artificially depleted plasma, ISI and INR values were too high. With TAS PT-NC systems, all three types of lyophilised plasma samples gave inaccurate ISI and unreliable INR results, similar to previous ECAA findings with fresh plasma calibrations. CONCLUSIONS: With CoaguChek Mini systems, ISI calibration can be simplified by the use of ECAA lyophilised plasma samples from coumarin treated patients. Further study is needed to devise a simpler calibration method for the TAS PT-NC system.
AIMS: The recommended method for the international sensitivity index (ISI) calibration of whole blood point of care testing (POCT) prothrombin time (PT) systems was originally described by Tripodi et al in 1993 but is too complex and demanding. The present European Concerted Action on Anticoagulation (ECAA) study aimed to assess the reliability of simpler ISI calibration using lyophilised plasma samples. METHODS: ISI calibrations using three different types of ECAA lyophilised plasma samples (artificially depleted, individual, and pooled coumarin) were compared with whole blood calibrations on CoaguChek Mini and TAS PT-NC POCT monitors at 10 centres. RESULTS: With CoaguChek Mini systems, lyophilised coumarin plasma samples (both single donation and pooled) gave ISI and international normalised ratio (INR) values comparable to whole blood. With artificially depleted plasma, ISI and INR values were too high. With TAS PT-NC systems, all three types of lyophilised plasma samples gave inaccurate ISI and unreliable INR results, similar to previous ECAA findings with fresh plasma calibrations. CONCLUSIONS: With CoaguChek Mini systems, ISI calibration can be simplified by the use of ECAA lyophilised plasma samples from coumarin treated patients. Further study is needed to devise a simpler calibration method for the TAS PT-NC system.
Authors: Leon Poller; Michelle Keown; Nikhil Chauhan; Anton M H P van den Besselaar; Joyce Meeuwisse-Braun; Armando Tripodi; Mariagrazia Clerici; Jørgen Jespersen Journal: Clin Chem Date: 2002-02 Impact factor: 8.327
Authors: L Poller; M Keown; N Chauhan; A M H P van Den Besselaar; A Tripodi; J Jespersen; C Shiach; M H Horellou; D Dias; N Egberg; J A Iriarte; I Kontopoulou-Griva; B Otridge Journal: Br J Haematol Date: 2002-03 Impact factor: 6.998
Authors: L Poller; M Keown; N Chauhan; A M H P van den Besselaar; A Tripodi; C Shiach; J Jespersen Journal: Thromb Haemost Date: 2002-05 Impact factor: 5.249
Authors: Leon Poller; Michelle Keown; Nikhil Chauhan; Anton M H P van den Besselaar; Armando Tripodi; Caroline Shiach; Jorgen Jespersen Journal: Clin Chem Date: 2002-10 Impact factor: 8.327
Authors: L Poller; T W Barrowcliffe; A M van den Besselaar; J Jespersen; A Tripodi; D Houghton Journal: Thromb Haemost Date: 1996-12 Impact factor: 5.249
Authors: Walter Ageno; Alexander S Gallus; Ann Wittkowsky; Mark Crowther; Elaine M Hylek; Gualtiero Palareti Journal: Chest Date: 2012-02 Impact factor: 9.410
Authors: Wendy S Baker; Kathleen J Albright; Heidi Spratt; Megan Berman; Peggy A Mann; Jaime Unabia; John R Petersen Journal: Data Brief Date: 2017-11-07