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Literature DB >> 11854495

Mechanical disruption of individual nucleosomes reveals a reversible multistage release of DNA.

Brent D Brower-Toland¹, Corey L Smith, Richard C Yeh, John T Lis, Craig L Peterson, Michelle D Wang.

Abstract

The dynamic structure of individual nucleosomes was examined by stretching nucleosomal arrays with a feedback-enhanced optical trap. Forced disassembly of each nucleosome occurred in three stages. Analysis of the data using a simple worm-like chain model yields 76 bp of DNA released from the histone core at low stretching force. Subsequently, 80 bp are released at higher forces in two stages: full extension of DNA with histones bound, followed by detachment of histones. When arrays were relaxed before the dissociated state was reached, nucleosomes were able to reassemble and to repeat the disassembly process. The kinetic parameters for nucleosome disassembly also have been determined.

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Year: 2002 PMID： 11854495 PMCID： PMC122302 DOI： 10.1073/pnas.022638399

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

27 in total

Mechanical disruption of individual nucleosomes reveals a reversible multistage release of DNA.

1. Effects of histone acetylation on the equilibrium accessibility of nucleosomal DNA target sites.

Review 2. The nucleosome core particle: does it have structural and physiologic relevance?

3. Sequence and position-dependence of the equilibrium accessibility of nucleosomal DNA target sites.

4. Pulling a single chromatin fiber reveals the forces that maintain its higher-order structure.

5. SWI/SNF chromatin remodeling requires changes in DNA topology.

6. Fast kinetics of chromatin assembly revealed by single-molecule videomicroscopy and scanning force microscopy.

7. Unfolding individual nucleosomes by stretching single chromatin fibers with optical tweezers.

Review 8. Probing the relation between force--lifetime--and chemistry in single molecular bonds.

9. Stepwise unfolding of titin under force-clamp atomic force microscopy.

10. Generation of superhelical torsion by ATP-dependent chromatin remodeling activities.

1. New insights into unwrapping DNA from the nucleosome from a single-molecule optical tweezers method.

Review 2. Optical tweezers stretching of chromatin.

Review 3. Micromechanical studies of mitotic chromosomes.

Review 4. Stretching and imaging single DNA molecules and chromatin.

5. Assembly of single chromatin fibers depends on the tension in the DNA molecule: magnetic tweezers study.

Review 6. Computational approaches to identify promoters and cis-regulatory elements in plant genomes.

7. Probing protein-DNA interactions by unzipping a single DNA double helix.

8. Packaging of single DNA molecules by the yeast mitochondrial protein Abf2p.

9. Direct observation of RuvAB-catalyzed branch migration of single Holliday junctions.

10. Single molecule elasticity measurements: a biophysical approach to bacterial nucleoid organization.