Literature DB >> 10074512

Mailed, home-obtained urine specimens: a reliable screening approach for detecting asymptomatic Chlamydia trachomatis infections.

S A Morré1, I G van Valkengoed, A de Jong, A J Boeke, J T van Eijk, C J Meijer, A J van den Brule.   

Abstract

The use of mailed, home-obtained urine specimens could facilitate screening programs for the detection of asymptomatic Chlamydia trachomatis infections. Since transport time could have an adverse effect on the sensitivity of C. trachomatis detection by PCR, the influence of DNA degradation on amplification was monitored over the course of 1 week. Therefore, urine specimens were aliquoted on the day of collection or arrival. Two groups of urine specimens were investigated. Group I contains first-void C. trachomatis-positive and -negative urine samples. DNA degradation was monitored in group I samples for 7 days at room temperature (RT) and at 4 degrees C by amplifying different lengths of the human beta-globin gene and the C. trachomatis plasmid target. DNA degradation was observed only for the larger human beta-globin fragments at days 5 to 7 at RT. In contrast, at 4 degrees C all targets could be amplified. Urine specimens were also frozen and thawed before aliquoting to mimic freezing during transport. This resulted in a lower sensitivity for the detection of C. trachomatis after thawing and 3 to 4 days at RT. In addition, mailed, home-obtained C. trachomatis-positive urine specimens (group II) were analyzed for 7 days after arrival by two commercially available C. trachomatis detection systems (PCR and ligase chain reaction [LCR]). The C. trachomatis plasmid target in mailed, home-obtained urine specimens could be amplified by both PCR and LCR after 1 week of storage and/or transport at RT. In conclusion, our findings indicate that mailed, home-obtained urine specimens are suitable for the sensitive detection of asymptomatic C. trachomatis infections by amplification methods, even if the transport time is up to 1 week at RT. These findings support the feasibility and validity of screening programs based on mailed, home-obtained urine specimens. Larger studies should be initiated to confirm our results.

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Year:  1999        PMID： 10074512      PMCID： PMC88635     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

1.  Effect of off-site transportation on detection of Neisseria gonorrhoeae in endocervical specimens.

Authors:  P C Iwen; R A Walker; K L Warren; D M Kelly; J Linder; S H Hinrichs
Journal:  Arch Pathol Lab Med       Date:  1996-11       Impact factor: 5.534

2.  Diagnosis of urogenital Chlamydia trachomatis infection in women based on mailed samples obtained at home: multipractice comparative study.

Authors:  L Ostergaard; J K Møller; B Andersen; F Olesen
Journal:  BMJ       Date:  1996-11-09

3.  RNA amplification by nucleic acid sequence-based amplification with an internal standard enables reliable detection of Chlamydia trachomatis in cervical scrapings and urine samples.

Authors:  S A Morré; P Sillekens; M V Jacobs; P van Aarle; S de Blok; B van Gemen; J M Walboomers; C J Meijer; A J van den Brule
Journal:  J Clin Microbiol       Date:  1996-12       Impact factor: 5.948

4.  A cost-effectiveness analysis of screening and treatment for Chlamydia trachomatis infection in asymptomatic women.

Authors:  M Genç; A Mårdh
Journal:  Ann Intern Med       Date:  1996-01-01       Impact factor: 25.391

5.  Diagnosis of Chlamydia trachomatis genitourinary infection in women by ligase chain reaction assay of urine.

Authors:  H H Lee; M A Chernesky; J Schachter; J D Burczak; W W Andrews; S Muldoon; G Leckie; W E Stamm
Journal:  Lancet       Date:  1995-01-28       Impact factor: 79.321

6.  Genotyping of Chlamydia trachomatis serovars derived from heterosexual partners and a detailed genomic analysis of serovar F.

Authors:  J Lan; C J Meijer; A R van den Hoek; J M Ossewaarde; J M Walboomers; A J van den Brule
Journal:  Genitourin Med       Date:  1995-10

7.  Prevention of pelvic inflammatory disease by screening for cervical chlamydial infection.

Authors:  D Scholes; A Stergachis; F E Heidrich; H Andrilla; K K Holmes; W E Stamm
Journal:  N Engl J Med       Date:  1996-05-23       Impact factor: 91.245

8.  Direct detection of Chlamydia trachomatis in urine specimens from symptomatic and asymptomatic men by using a rapid polymerase chain reaction assay.

Authors:  G Jaschek; C A Gaydos; L E Welsh; T C Quinn
Journal:  J Clin Microbiol       Date:  1993-05       Impact factor: 5.948

9.  Genital Chlamydia trachomatis infections in Uppsala County, Sweden, 1985-1993: declining rates for how much longer?

Authors:  B Herrmann; M Egger
Journal:  Sex Transm Dis       Date:  1995 Jul-Aug       Impact factor: 2.830

10.  Processing of long-stored archival cervical smears for human papillomavirus detection by the polymerase chain reaction.

Authors:  A M de Roda Husman; P J Snijders; H V Stel; A J van den Brule; C J Meijer; J M Walboomers
Journal:  Br J Cancer       Date:  1995-08       Impact factor: 7.640
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  16 in total

1.  Pooling of urine specimens for detection of asymptomatic Chlamydia trachomatis infections by PCR in a low-prevalence population: cost-saving strategy for epidemiological studies and screening programs.

Authors:  S A Morré; C J Meijer; C Munk; S Krüger-Kjaer; J F Winther; H O Jørgensens; A J van Den Brule
Journal:  J Clin Microbiol       Date:  2000-04       Impact factor: 5.948

2.  Pooling cervical swabs for detection of Chlamydia trachomatis by PCR: sensitivity, dilution, inhibition, and cost-saving aspects.

Authors:  S A Morré; R van Dijk; C J Meijer; A J van den Brule; S K Kjaer; C Munk
Journal:  J Clin Microbiol       Date:  2001-06       Impact factor: 5.948

3.  Prospective comparison of cell cultures and nucleic acid amplification tests for laboratory diagnosis of Chlamydia trachomatis Infections.

Authors:  Deborah J Jespersen; Karen S Flatten; Mary F Jones; Thomas F Smith
Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

Review 4.  Home versus clinic-based specimen collection for Chlamydia trachomatis and Neisseria gonorrhoeae.

Authors:  Anna S Graseck; Shirley L Shih; Jeffrey F Peipert
Journal:  Expert Rev Anti Infect Ther       Date:  2011-02       Impact factor: 5.091

5.  Cost effectiveness analysis of a population based screening programme for asymptomatic Chlamydia trachomatis infections in women by means of home obtained urine specimens.

Authors:  I G van Valkengoed; M J Postma; S A Morré; A J van den Brule; C J Meijer; L M Bouter; A J Boeke
Journal:  Sex Transm Infect       Date:  2001-08       Impact factor: 3.519

6.  A prediction rule for selective screening of Chlamydia trachomatis infection.

Authors:  H M Götz; J E A M van Bergen; I K Veldhuijzen; J Broer; C J P A Hoebe; E W Steyerberg; A J J Coenen; F de Groot; M J C Verhooren; D T van Schaik; J H Richardus
Journal:  Sex Transm Infect       Date:  2005-02       Impact factor: 3.519

7.  Low diagnostic accuracy of selective screening criteria for asymptomatic Chlamydia trachomatis infections in the general population.

Authors:  I G van Valkengoed; S A Morré; A J van den Brule; C J Meijer; W Devillé; L M Bouter; A J Boeke
Journal:  Sex Transm Infect       Date:  2000-10       Impact factor: 3.519

8.  Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR.

Authors:  Laura van Dommelen; Petra F G Wolffs; Frank H van Tiel; Nicole Dukers; Selma B Herngreen; Cathrien A Bruggeman; Christian J P A Hoebe
Journal:  J Clin Microbiol       Date:  2012-12-19       Impact factor: 5.948

9.  Follow-up, treatment, and reinfection rates among asymptomatic chlamydia trachomatis cases in general practice.

Authors:  Irene G M van Valkengoed; Servaas A Morré; Adriaan J C van den Brule; Chris J L M Meijer; Lex M Bouter; Jacques Th M van Eijk; A Joan P Boeke
Journal:  Br J Gen Pract       Date:  2002-08       Impact factor: 5.386

10.  Implications of storing urinary DNA from different populations for molecular analyses.

Authors:  Angela Cannas; Glendah Kalunga; Clare Green; Ludovica Calvo; Patrick Katemangwe; Klaus Reither; Mark D Perkins; Leonard Maboko; Michael Hoelscher; Elizabeth A Talbot; Peter Mwaba; Alimuddin I Zumla; Enrico Girardi; Jim F Huggett
Journal:  PLoS One       Date:  2009-09-10       Impact factor: 3.240
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