Literature DB >> 16208009

Prospective comparison of cell cultures and nucleic acid amplification tests for laboratory diagnosis of Chlamydia trachomatis Infections.

Deborah J Jespersen1, Karen S Flatten, Mary F Jones, Thomas F Smith.   

Abstract

Specimens submitted in M5 medium for cell culture detection of Chlamydia trachomatis were tested by nucleic acid amplification testing (NAAT) and in cell cultures. Of 35 (genital) and 26 (nongenital) specimens positive for C. trachomatis, 21 and 14 specimens, respectively, were detected exclusively by NAAT. NAAT is significantly (P<0.0001) more sensitive than cell culture and should be considered the new "gold standard" for the laboratory diagnosis of C. trachomatis infections.

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Year:  2005        PMID： 16208009      PMCID： PMC1248517          DOI： 10.1128/JCM.43.10.5324-5326.2005

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  16 in total

Review 1.  The accuracy and efficacy of screening tests for Chlamydia trachomatis: a systematic review.

Authors:  Emma J Watson; Allan Templeton; Ian Russell; Jorma Paavonen; Per-Anders Mardh; Angelika Stary; Babil Stray Pederson
Journal:  J Med Microbiol       Date:  2002-12       Impact factor: 2.472

2.  Use of sequential enzyme immunoassay and direct fluorescent antibody tests for detection of Chlamydia trachomatis infections in women.

Authors:  J R Schwebke; W E Stamm; H H Handsfield
Journal:  J Clin Microbiol       Date:  1990-11       Impact factor: 5.948

Review 3.  Laboratory techniques for the diagnosis of chlamydial infections.

Authors:  D Taylor-Robinson; B J Thomas
Journal:  Genitourin Med       Date:  1991-06

Review 4.  Tests for infection with Chlamydia trachomatis.

Authors:  D Taylor-Robinson
Journal:  Int J STD AIDS       Date:  1996 Jan-Feb       Impact factor: 1.359

5.  Accuracy of Chlamydia trachomatis antigen detection methods in a low-prevalence population in a primary care setting.

Authors:  P H Gann; J E Herrmann; L Candib; R W Hudson
Journal:  J Clin Microbiol       Date:  1990-07       Impact factor: 5.948

6.  Screening tests to detect Chlamydia trachomatis and Neisseria gonorrhoeae infections--2002.

Authors:  Robert E Johnson; Wilbert J Newhall; John R Papp; Joan S Knapp; Carolyn M Black; Thomas L Gift; Richard Steece; Lauri E Markowitz; Owen J Devine; Cathleen M Walsh; Susan Wang; Dorothy C Gunter; Kathleen L Irwin; Susan DeLisle; Stuart M Berman
Journal:  MMWR Recomm Rep       Date:  2002-10-18

7.  Comparison of iodine- and fluorescein-labeled monoclonal antibodies for detection of Chlamydia trachomatis inclusions in cells grown in glass vials.

Authors:  D J Wilson; T F Smith; D M Ilstrup
Journal:  Diagn Microbiol Infect Dis       Date:  1984-01       Impact factor: 2.803

8.  Evaluation of the Amplicor Chlamydia trachomatis test versus culture in genital samples in various prevalence populations.

Authors:  B de Barbeyrac; I Pellet; B Dutilh; C Bébéar; B Dumon; M Géniaux; C Bébéar
Journal:  Genitourin Med       Date:  1994-06

9.  Comparison of the APTIMA CT and GC assays with the APTIMA combo 2 assay, the Abbott LCx assay, and direct fluorescent-antibody and culture assays for detection of Chlamydia trachomatis and Neisseria gonorrhoeae.

Authors:  B Boyadzhyan; T Yashina; J H Yatabe; M Patnaik; C S Hill
Journal:  J Clin Microbiol       Date:  2004-07       Impact factor: 5.948

10.  Use of multiple nucleic acid amplification tests to define the infected-patient "gold standard" in clinical trials of new diagnostic tests for Chlamydia trachomatis infections.

Authors:  David H Martin; Malanda Nsuami; Julius Schachter; Edward W Hook; Dennis Ferrero; Thomas C Quinn; Charlotte Gaydos
Journal:  J Clin Microbiol       Date:  2004-10       Impact factor: 5.948
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  3 in total

1.  Randomized controlled trial of the relative efficacy of high-dose intravenous ceftriaxone and oral cefixime combined with doxycycline for the treatment of Chlamydia trachomatis and Neisseria gonorrhoeae co-infection.

Authors:  Phuong Thi Thu Nguyen; Ha Viet Pham; Dung Hoang Van; Linh Van Pham; Hoi Thanh Nguyen; Hung Van Nguyen
Journal:  BMC Infect Dis       Date:  2022-07-09       Impact factor: 3.667

Review 2.  Chlamydia trachomatis today: treatment, detection, immunogenetics and the need for a greater global understanding of chlamydial disease pathogenesis.

Authors:  D Dean
Journal:  Drugs Today (Barc)       Date:  2009-11       Impact factor: 2.245

3.  In-cell western assay as a high-throughput approach for Chlamydia trachomatis quantification and susceptibility testing to antimicrobials.

Authors:  Simone Filardo; Marisa Di Pietro; Patrizio Pasqualetti; Martina Manera; Fabiana Diaco; Rosa Sessa
Journal:  PLoS One       Date:  2021-05-11       Impact factor: 3.240
  3 in total

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