Literature DB >> 23254134

Influence of temperature, medium, and storage duration on Chlamydia trachomatis DNA detection by PCR.

Laura van Dommelen1, Petra F G Wolffs, Frank H van Tiel, Nicole Dukers, Selma B Herngreen, Cathrien A Bruggeman, Christian J P A Hoebe.   

Abstract

We validated the use of stored samples for Chlamydia trachomatis research. C. trachomatis DNA was detected by real-time PCR in clinical (urine and self-taken vaginal swabs) and spiked samples using six different media, five different time points (up to 2 years), and four different temperature conditions. C. trachomatis was detected in all 423 samples, and no clinically relevant degradation impact was detected.

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Year:  2012        PMID： 23254134      PMCID： PMC3592091          DOI： 10.1128/JCM.02631-12

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

1.  Mailed, home-obtained urine specimens: a reliable screening approach for detecting asymptomatic Chlamydia trachomatis infections.

Authors:  S A Morré; I G van Valkengoed; A de Jong; A J Boeke; J T van Eijk; C J Meijer; A J van den Brule
Journal:  J Clin Microbiol       Date:  1999-04       Impact factor: 5.948

2.  Development and validation of a rotor-gene real-time PCR assay for detection, identification, and quantification of Chlamydia trachomatis in a single reaction.

Authors:  Hamid Jalal; Hannah Stephen; Martin D Curran; Janet Burton; Michelle Bradley; Christopher Carne
Journal:  J Clin Microbiol       Date:  2006-01       Impact factor: 5.948

3.  Lessons learned from a population-based chlamydia screening pilot.

Authors:  Hannelore M Götz; Jan E A M van Bergen; Irene K Veldhuijzen; Christian J P A Hoebe; Jan Broer; A J J Coenen; F de Groot; M J C Verhooren; D T van Schaik; Jan H Richardus
Journal:  Int J STD AIDS       Date:  2006-12       Impact factor: 1.359

4.  Effect of swab type and storage temperature on the isolation of Chlamydia trachomatis from clinical specimens.

Authors:  J B Mahony; M A Chernesky
Journal:  J Clin Microbiol       Date:  1985-11       Impact factor: 5.948

5.  Effect of storage temperature on survival of Chlamydia trachomatis after lyophilization.

Authors:  Adrian Eley; Ian Geary; Abbas Bahador; Hamid Hakimi
Journal:  J Clin Microbiol       Date:  2006-07       Impact factor: 5.948

6.  Transport and storage conditions for cultural recovery of Chlamydia pneumoniae.

Authors:  M Maass; K Dalhoff
Journal:  J Clin Microbiol       Date:  1995-07       Impact factor: 5.948

7.  Survival of Chlamydia trachomatis in different transport media and at different temperatures: diagnostic implications.

Authors:  K H Tjiam; B Y van Heijst; J C de Roo; A de Beer; T van Joost; M F Michel; E Stolz
Journal:  Br J Vener Dis       Date:  1984-04

8.  TaqMan assay for Swedish Chlamydia trachomatis variant.

Authors:  Arnold Catsburg; Laura van Dommelen; Vitaly Smelov; Henry J C de Vries; Alevtina Savitcheva; Marius Domeika; Björn Herrmann; Sander Ouburg; Christian J P A Hoebe; Anders Nilsson; Paul H M Savelkoul; Servaas A Morré
Journal:  Emerg Infect Dis       Date:  2007-09       Impact factor: 6.883

9.  Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon.

Authors:  Jim F Huggett; Tanya Novak; Jeremy A Garson; Clare Green; Stephen D Morris-Jones; Robert F Miller; Alimuddin Zumla
Journal:  BMC Res Notes       Date:  2008-08-28
  9 in total
  11 in total

1.  Microbiological Characteristics of Chlamydia trachomatis and Neisseria gonorrhoeae Infections in South African Women.

Authors:  Jan Henk Dubbink; Dewi J de Waaij; Myrte Bos; Lisette van der Eem; Cécile Bébéar; Nontembeko Mbambazela; Sander Ouburg; Remco P H Peters; Servaas A Morré
Journal:  J Clin Microbiol       Date:  2015-10-28       Impact factor: 5.948

2.  Fluorescent Biosensor Based on Hairpin DNA Stabilized Copper Nanoclusters for Chlamydia trachomatis Detection.

Authors:  Luyao Liu; Qinqin Bai; Xuebing Zhang; Chunxue Lu; Zhongyu Li; Hao Liang; Lili Chen
Journal:  J Fluoresc       Date:  2022-05-25       Impact factor: 2.525

3.  Despite Excellent Test Characteristics of the cobas 4800 CT/NG Assay, Detection of Oropharyngeal Chlamydia trachomatis and Neisseria gonorrhoeae Remains Challenging.

Authors:  J M van Niekerk; B M J W van der Veer; C J P A Hoebe; J van de Bovenkamp; C van Herk; I H M van Loo; L B van Alphen; P F G Wolffs
Journal:  J Clin Microbiol       Date:  2021-01-21       Impact factor: 5.948

4.  Influence of storage time on DNA of Chlamydia trachomatis, Ureaplasma urealyticum, and Neisseria gonorrhoeae for accurate detection by quantitative real-time polymerase chain reaction.

Authors:  Y Lu; C Z Rong; J Y Zhao; X J Lao; L Xie; S Li; X Qin
Journal:  Braz J Med Biol Res       Date:  2016-08-25       Impact factor: 2.590

5.  Reduced-cost Chlamydia trachomatis-specific multiplex real-time PCR diagnostic assay evaluated for ocular swabs and use by trachoma research programmes.

Authors:  Robert Butcher; Jo Houghton; Tamsyn Derrick; Athumani Ramadhani; Beatriz Herrera; Anna R Last; Patrick A Massae; Matthew J Burton; Martin J Holland; Chrissy H Roberts
Journal:  J Microbiol Methods       Date:  2017-05-06       Impact factor: 2.363

6.  Chlamydia trachomatis intra-bacterial and total plasmid copy number in clinical urogenital samples.

Authors:  J A M C Dirks; K Janssen; C J P A Hoebe; T H B Geelen; M Lucchesi; N H T M Dukers-Muijrers; P F G Wolffs
Journal:  Sci Rep       Date:  2021-01-08       Impact factor: 4.379

7.  Higher organism load associated with failure of azithromycin to treat rectal chlamydia.

Authors:  F Y S Kong; S N Tabrizi; C K Fairley; S Phillips; G Fehler; M Law; L A Vodstrcil; M Chen; C S Bradshaw; J S Hocking
Journal:  Epidemiol Infect       Date:  2016-05-16       Impact factor: 4.434

8.  A 30-Min Nucleic Acid Amplification Point-of-Care Test for Genital Chlamydia trachomatis Infection in Women: A Prospective, Multi-center Study of Diagnostic Accuracy.

Authors:  E M Harding-Esch; E C Cousins; S-L C Chow; L T Phillips; C L Hall; N Cooper; S S Fuller; A V Nori; R Patel; S Thomas-William; G Whitlock; S J E Edwards; M Green; J Clarkson; B Arlett; J K Dunbar; C M Lowndes; S T Sadiq
Journal:  EBioMedicine       Date:  2018-01-10       Impact factor: 8.143

9.  Ocular Chlamydia trachomatis Infection Under the Surgery, Antibiotics, Facial Cleanliness, and Environmental Improvement Strategy in Amhara, Ethiopia, 2011-2015.

Authors:  Scott D Nash; Aisha E P Stewart; Mulat Zerihun; Eshetu Sata; Demelash Gessese; Berhanu Melak; Tekola Endeshaw; Melsew Chanyalew; Ambahun Chernet; Belay Bayissasse; Jeanne Moncada; Thomas M Lietman; Paul M Emerson; Jonathan D King; Zerihun Tadesse; E Kelly Callahan
Journal:  Clin Infect Dis       Date:  2018-11-28       Impact factor: 9.079

10.  Diagnostic accuracy of a prototype rapid chlamydia and gonorrhoea recombinase polymerase amplification assay: a multicentre cross-sectional preclinical evaluation.

Authors:  E M Harding-Esch; S S Fuller; S-L C Chow; A V Nori; M A Harrison; M Parker; O Piepenburg; M S Forrest; D G Brooks; R Patel; P E Hay; N Fearnley; M J Pond; J K Dunbar; P D Butcher; T Planche; C M Lowndes; S T Sadiq
Journal:  Clin Microbiol Infect       Date:  2018-06-12       Impact factor: 8.067
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