Literature DB >> 9862131

3D HCCH-COSY-TOCSY experiment for the assignment of ribose and amino acid side chains in 13C labeled RNA and protein.

W Hu1, L T Kakalis, L Jiang, F Jiang, X Ye, A Majumdar.   

Abstract

A new 3D HCCH-COSY-TOCSY experiment is presented for the assignment of RNA sugar and protein side chains. The experiment, which combines COSY and TOCSY units, is more powerful than the sum of individual HCCH-COSY and HCCH-TOCSY pulse sequences. The experiment was applied to a 13C, 15N-labeled 26 mer RNA complexed with the antibiotic tobramycin, and a 12 kDa 13C, 15N-labeled FKBP12 protein sample. The power of HCCH-COSY-TOCSY is demonstrated through complete spin system assignments of sugars in the 26 mer RNA sample, which could not be assigned using a combination of HCCH-COSY, HCCH-TOCSY and 13C-edited NOESY experiments.

Entities:  

Mesh：

Substances：

Year:  1998        PMID： 9862131     DOI： 10.1023/a:1008365301124

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  10 in total

1.  Directed TOCSY, a method for selection of directed correlations by optimal combinations of isotropic and longitudinal mixing.

Authors:  S J Glaser; H Schwalbe; J P Marino; C Griesinger
Journal:  J Magn Reson B       Date:  1996-08

2.  NMR View: A computer program for the visualization and analysis of NMR data.

Authors:  B A Johnson; R A Blevins
Journal:  J Biomol NMR       Date:  1994-09       Impact factor: 2.835

3.  NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors:  F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
Journal:  J Biomol NMR       Date:  1995-11       Impact factor: 2.835

4.  Specific binding of aminoglycoside antibiotics to RNA.

Authors:  Y Wang; R R Rando
Journal:  Chem Biol       Date:  1995-05

5.  Proton and nitrogen sequential assignments and secondary structure determination of the human FK506 and rapamycin binding protein.

Authors:  M K Rosen; S W Michnick; M Karplus; S L Schreiber
Journal:  Biochemistry       Date:  1991-05-14       Impact factor: 3.162

6.  1H, 13C, and 15N assignments and secondary structure of the FK506 binding protein when bound to ascomycin.

Authors:  R X Xu; D Nettesheim; E T Olejniczak; R Meadows; G Gemmecker; S W Fesik
Journal:  Biopolymers       Date:  1993-04       Impact factor: 2.505

7.  Improved three-dimensional 1H-13C-1H correlation spectroscopy of a 13C-labeled protein using constant-time evolution.

Authors:  M Ikura; L E Kay; A Bax
Journal:  J Biomol NMR       Date:  1991-09       Impact factor: 2.835

8.  A 4D HCCH-TOCSY experiment for assigning the side chain 1H and 13C resonances of proteins.

Authors:  E T Olejniczak; R X Xu; S W Fesik
Journal:  J Biomol NMR       Date:  1992-11       Impact factor: 2.835

9.  Amino acid type determination in the sequential assignment procedure of uniformly 13C/15N-enriched proteins.

Authors:  S Grzesiek; A Bax
Journal:  J Biomol NMR       Date:  1993-03       Impact factor: 2.835

10.  A general method for assigning NMR spectra of denatured proteins using 3D HC(CO)NH-TOCSY triple resonance experiments.

Authors:  T M Logan; E T Olejniczak; R X Xu; S W Fesik
Journal:  J Biomol NMR       Date:  1993-03       Impact factor: 2.835
  10 in total
  8 in total

1.  A single-nucleotide natural variation (U4 to C4) in an influenza A virus promoter exhibits a large structural change: implications for differential viral RNA synthesis by RNA-dependent RNA polymerase.

Authors:  Mi-Kyung Lee; Sung-Hun Bae; Chin-Ju Park; Hae-Kap Cheong; Chaejoon Cheong; Byong-Seok Choi
Journal:  Nucleic Acids Res       Date:  2003-02-15       Impact factor: 16.971

2.  Structural mimicry in the phage phi21 N peptide-boxB RNA complex.

Authors:  Christopher D Cilley; James R Williamson
Journal:  RNA       Date:  2003-06       Impact factor: 4.942

3.  New applications of 2D filtered/edited NOESY for assignment and structure elucidation of RNA and RNA-protein complexes.

Authors:  Robert D Peterson; Carla A Theimer; Haihong Wu; Juli Feigon
Journal:  J Biomol NMR       Date:  2004-01       Impact factor: 2.835

4.  Multiple-quantum HCN-CCH-TOCSY experiment for 13C/15N labeled RNA oligonucleotides.

Authors:  W Hu; L Jiang
Journal:  J Biomol NMR       Date:  1999-12       Impact factor: 2.835

5.  Structure of the K-turn U4 RNA: a combined NMR and SANS study.

Authors:  Melanie Falb; Irene Amata; Frank Gabel; Bernd Simon; Teresa Carlomagno
Journal:  Nucleic Acids Res       Date:  2010-05-13       Impact factor: 16.971

6.  Solution structure of the influenza A virus cRNA promoter: implications for differential recognition of viral promoter structures by RNA-dependent RNA polymerase.

Authors:  Chin-Ju Park; Sung-Hun Bae; Mi-Kyung Lee; Gabriele Varani; Byong-Seok Choi
Journal:  Nucleic Acids Res       Date:  2003-06-01       Impact factor: 16.971

7.  The structure of the SOLE element of oskar mRNA.

Authors:  Bernd Simon; Pawel Masiewicz; Anne Ephrussi; Teresa Carlomagno
Journal:  RNA       Date:  2015-06-18       Impact factor: 4.942

8.  Studying intact bacterial peptidoglycan by proton-detected NMR spectroscopy at 100 kHz MAS frequency.

Authors:  Catherine Bougault; Isabel Ayala; Waldemar Vollmer; Jean-Pierre Simorre; Paul Schanda
Journal:  J Struct Biol       Date:  2018-07-19       Impact factor: 2.867
  8 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.