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Literature DB >> 10685339

Multiple-quantum HCN-CCH-TOCSY experiment for 13C/15N labeled RNA oligonucleotides.

Abstract

A multiple-quantum 3D HCN-CCH-TOCSY experiment is presented for the assignment of RNA ribose resonances. The experiment makes use of the chemical shift dispersion of N1 of pyrimidine and N9 of purine to distinguish the ribose spin systems. It provides an alternative approach for the assignment of ribose resonances to the currently used COSY- and TOCSY-type experiments in which either 13C or 1H is utilized to distinguish the different spin systems. Compared to the single-quantum version, the sensitivity of the multiple-quantum HCN-CCH-TOCSY experiment is enhanced on average by a factor of 2 for a 23-mer RNA aptamer complexed with neomycin.

Entities: Chemical Gene

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Year: 1999 PMID： 10685339 DOI： 10.1023/a:1008391007013

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

12 in total

1. New applications of 2D filtered/edited NOESY for assignment and structure elucidation of RNA and RNA-protein complexes.

Authors: Robert D Peterson; Carla A Theimer; Haihong Wu; Juli Feigon
Journal: J Biomol NMR Date: 2004-01 Impact factor: 2.835

2. Novel 2D and 3D multiple-quantum bi-directional HCNCH experiments for the correlation of ribose and base protons/carbons in 13C/15N labeled RNA.

Authors: W Hu; Y Q Gosser; W Xu; D J Patel
Journal: J Biomol NMR Date: 2001-06 Impact factor: 2.835

2 in total

Multiple-quantum HCN-CCH-TOCSY experiment for 13C/15N labeled RNA oligonucleotides.

1. 3D HCCH-COSY-TOCSY experiment for the assignment of ribose and amino acid side chains in 13C labeled RNA and protein.

2. NMR View: A computer program for the visualization and analysis of NMR data.

3. NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

4. Saccharide-RNA recognition in a complex formed between neomycin B and an RNA aptamer.

5. A novel RNA motif for neomycin recognition.

6. Improved three-dimensional 1H-13C-1H correlation spectroscopy of a 13C-labeled protein using constant-time evolution.

7. An efficient procedure for assignment of the proton, carbon and nitrogen resonances in 13C/15N labeled nucleic acids.

8. Spin-locked multiple quantum coherence for signal enhancement in heteronuclear multidimensional NMR experiments.

9. A 4D HCCH-TOCSY experiment for assigning the side chain 1H and 13C resonances of proteins.

10. Sequential assignments in uniformly 13C- and 15N-labelled RNAs: the HC(N,P) and HC(N,P)-CCH-TOCSY experiments.

1. New applications of 2D filtered/edited NOESY for assignment and structure elucidation of RNA and RNA-protein complexes.

2. Novel 2D and 3D multiple-quantum bi-directional HCNCH experiments for the correlation of ribose and base protons/carbons in 13C/15N labeled RNA.