Literature DB >> 1841700

Improved three-dimensional 1H-13C-1H correlation spectroscopy of a 13C-labeled protein using constant-time evolution.

M Ikura1, L E Kay, A Bax.   

Abstract

An improved version of the three-dimensional HCCH-COSY NMR experiment is described that correlates the resonances of geminal and vicinal proton pairs with the chemical shift of the 13C nucleus attached to one of the protons. The experiment uses constant-time evolution of transverse 13C magnetization which optimizes transfer of magnetization and thus improves the sensitivity of the experiment over the original scheme. The experiment is demonstrated for calmodulin complexed with a 26-residue peptide comprising the binding site of skeletal muscle myosin light chain kinase.

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Year:  1991        PMID： 1841700     DOI： 10.1007/bf01875522

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  49 in total

1.  Assignment of selectively 13C-labeled cellopentaose synthesized using an engineered glycosynthase.

Authors:  E Brun; H Brumer; L F MacKenzie; S G Withers; L P McIntosh
Journal:  J Biomol NMR       Date:  2001-09       Impact factor: 2.835

2.  Solution structure of a yeast ubiquitin-like protein Smt3: the role of structurally less defined sequences in protein-protein recognitions.

Authors:  Wanyun Sheng; Xiubei Liao
Journal:  Protein Sci       Date:  2002-06       Impact factor: 6.725

3.  Polarization transfer by cross-correlated relaxation in solution NMR with very large molecules.

Authors:  R Riek; G Wider; K Pervushin; K Wüthrich
Journal:  Proc Natl Acad Sci U S A       Date:  1999-04-27       Impact factor: 11.205

4.  Solution structure of the epsin N-terminal homology (ENTH) domain of human epsin.

Authors:  Seizo Koshiba; Takanori Kigawa; Akira Kikuchi; Shigeyuki Yokoyama
Journal:  J Struct Funct Genomics       Date:  2002

5.  Measurement of two-bond JCOH alpha coupling constants in proteins uniformly enriched with 13C.

Authors:  G W Vuister; A Bax
Journal:  J Biomol NMR       Date:  1992-07       Impact factor: 2.835

6.  Three-dimensional deuterium-carbon correlation experiments for high-resolution solid-state MAS NMR spectroscopy of large proteins.

Authors:  Daniela Lalli; Paul Schanda; Anup Chowdhury; Joren Retel; Matthias Hiller; Victoria A Higman; Lieselotte Handel; Vipin Agarwal; Bernd Reif; Barth van Rossum; Umit Akbey; Hartmut Oschkinat
Journal:  J Biomol NMR       Date:  2011-10-25       Impact factor: 2.835

7.  Tools for the automated assignment of high-resolution three-dimensional protein NMR spectra based on pattern recognition techniques.

Authors:  D Croft; J Kemmink; K P Neidig; H Oschkinat
Journal:  J Biomol NMR       Date:  1997-10       Impact factor: 2.835

8.  A conserved spider silk domain acts as a molecular switch that controls fibre assembly.

Authors:  Franz Hagn; Lukas Eisoldt; John G Hardy; Charlotte Vendrely; Murray Coles; Thomas Scheibel; Horst Kessler
Journal:  Nature       Date:  2010-05-13       Impact factor: 49.962

9.  A modified strategy for sequence specific assignment of protein NMR spectra based on amino acid type selective experiments.

Authors:  Mario Schubert; Dirk Labudde; Dietmar Leitner; Hartmut Oschkinat; Peter Schmieder
Journal:  J Biomol NMR       Date:  2005-02       Impact factor: 2.835

10.  High-resolution NMR characterization of a spider-silk mimetic composed of 15 tandem repeats and a CRGD motif.

Authors:  Glendon D McLachlan; Joseph Slocik; Robert Mantz; David Kaplan; Sean Cahill; Mark Girvin; Steve Greenbaum
Journal:  Protein Sci       Date:  2009-01       Impact factor: 6.725
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