Literature DB >> 9431965

Inhibition of PCR by agar from bacteriological transport media.

A P Gibb1, S Wong.   

Abstract

We observed inhibition of PCR in throat swabs submitted in routine bacteriological transport media. Experimental studies showed that agar, which was extracted with DNA by DNAzol (Gibco BRL, Gaithersburg, Md.), was the inhibitory agent. No inhibitory effect was observed with a transport medium which did not contain agar.

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Year:  1998        PMID： 9431965      PMCID： PMC124852     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  A modified formula for the preparation of Stuart's Transport Medium.

Authors:  C R Amies
Journal:  Can J Public Health       Date:  1967-07

2.  Comparison of nasopharyngeal aspirates and throat swab specimens in a polymerase chain reaction-based test for Mycoplasma pneumoniae.

Authors:  M Reznikov; T K Blackmore; J J Finlay-Jones; D L Gordon
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1995-01       Impact factor: 3.267

3.  Inhibition of the polymerase chain reaction by mucolytic agents.

Authors:  H G Deneer; I Knight
Journal:  Clin Chem       Date:  1994-01       Impact factor: 8.327

4.  Inhibition of PCR-based assay for Bordetella pertussis by using calcium alginate fiber and aluminum shaft components of a nasopharyngeal swab.

Authors:  R M Wadowsky; S Laus; T Libert; S J States; G D Ehrlich
Journal:  J Clin Microbiol       Date:  1994-04       Impact factor: 5.948

5.  Detection of Mycoplasma pneumoniae in clinical samples from pediatric patients by polymerase chain reaction.

Authors:  L Skakni; A Sardet; J Just; J Landman-Parker; J Costil; N Moniot-Ville; F Bricout; A Garbarg-Chenon
Journal:  J Clin Microbiol       Date:  1992-10       Impact factor: 5.948

6.  Direct PCR enables detection of Mycoplasma pneumoniae in patients with respiratory tract infections.

Authors:  J H Tjhie; F J van Kuppeveld; R Roosendaal; W J Melchers; R Gordijn; D M MacLaren; J M Walboomers; C J Meijer; A J van den Brule
Journal:  J Clin Microbiol       Date:  1994-01       Impact factor: 5.948

7.  Detection of Mycoplasma pneumoniae by polymerase chain reaction and nonradioactive hybridization in microtiter plates.

Authors:  E Lüneberg; J S Jensen; M Frosch
Journal:  J Clin Microbiol       Date:  1993-05       Impact factor: 5.948
  7 in total
  14 in total

Review 1.  Molecular diagnosis of Mycoplasma pneumoniae respiratory tract infections.

Authors:  K Loens; D Ursi; H Goossens; M Ieven
Journal:  J Clin Microbiol       Date:  2003-11       Impact factor: 5.948

2.  Development and use of an internal positive control for detection of Bordetella pertussis by PCR.

Authors:  Stéphanie Herwegh; Christophe Carnoy; Frédéric Wallet; Caroline Loïez; René J Courcol
Journal:  J Clin Microbiol       Date:  2005-05       Impact factor: 5.948

3.  Evaluation of the BD Max MRSA XT assay for use with different swab types.

Authors:  Alexander H Dalpke; Marjeta Hofko; Christian Stock; Stefan Zimmermann
Journal:  J Clin Microbiol       Date:  2014-09-17       Impact factor: 5.948

4.  Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories.

Authors:  Kathy L Toohey-Kurth; Donna M Mulrooney; Susanne Hinkley; Mary Lea Killian; Janice C Pedersen; Mangkey A Bounpheng; Roman Pogranichniy; Steve Bolin; Roger Maes; Rebecca L Tallmadge; Laura B Goodman; Beate M Crossley
Journal:  J Vet Diagn Invest       Date:  2020-09-30       Impact factor: 1.279

5.  Inhibition monitoring in veterinary molecular testing.

Authors:  Lifang Yan; Kathy L Toohey-Kurth; Beate M Crossley; Jianfa Bai; Amy L Glaser; Rebecca L Tallmadge; Laura B Goodman
Journal:  J Vet Diagn Invest       Date:  2019-11-18       Impact factor: 1.279

6.  Comparison of culture versus quantitative real-time polymerase chain reaction for the detection of Taylorella equigenitalis in field samples from naturally infected horses in Canada and Germany.

Authors:  Susan Nadin-Davis; Margaret K Knowles; Teresa Burke; Reinhard Böse; John Devenish
Journal:  Can J Vet Res       Date:  2015-07       Impact factor: 1.310

7.  High rate of N2 fixation by East Siberian cryophilic soil bacteria as determined by measuring acetylene reduction in nitrogen-poor medium solidified with gellan gum.

Authors:  Shintaro Hara; Yasuyuki Hashidoko; Roman V Desyatkin; Ryusuke Hatano; Satoshi Tahara
Journal:  Appl Environ Microbiol       Date:  2009-03-13       Impact factor: 4.792

8.  Use of CultureSwab Plus swabs with Amies gel agar for testing of naris specimens with the GeneOhm MRSA assay.

Authors:  P P Bourbeau; J A Riley; B C Shoemaker; K S Jones
Journal:  J Clin Microbiol       Date:  2007-05-09       Impact factor: 5.948

9.  Development of an optimized method for the detection of airborne viruses with real-time PCR analysis.

Authors:  Panos G Ziros; Petros A Kokkinos; Euaggelia Legaki; Apostolos Vantarakis
Journal:  Virol J       Date:  2011-07-27       Impact factor: 4.099

10.  Performance of transport and selective media for swine Bordetella bronchiseptica recovery and it comparison to polymerase chain reaction detection.

Authors:  Tania Alen Coutinho; Mari Lourdes Bernardi; Marisa Ribeiro de Itapema Cardoso; Sandra Maria Borowski; Andrea Micke Moreno; David Emilio Santos Neves de Barcellos
Journal:  Braz J Microbiol       Date:  2009-09-01       Impact factor: 2.476
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