Literature DB >> 32996402

Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories.

Kathy L Toohey-Kurth1, Donna M Mulrooney2, Susanne Hinkley3, Mary Lea Killian4, Janice C Pedersen4, Mangkey A Bounpheng5, Roman Pogranichniy6, Steve Bolin7, Roger Maes7, Rebecca L Tallmadge8, Laura B Goodman8, Beate M Crossley9.   

Abstract

The exquisite sensitivity of in vitro amplification assays such as real-time polymerase chain reaction (rtPCR) requires the establishment of thorough and robust laboratory practices. To this end, an American Association of Veterinary Laboratory Diagnosticians (AAVLD) committee of subject matter experts was convened to develop a set of best practices for performance of nucleic acid amplification assays. Consensus advice for the performance of preanalytical, analytical, and postanalytical steps is presented here, along with a review of supporting literature.

Keywords:  AAVLD; practice guidelines; qPCR; real-time PCR

Mesh：

Year:  2020        PMID： 32996402      PMCID： PMC7649542          DOI： 10.1177/1040638720962076

Source DB:  PubMed          Journal:  J Vet Diagn Invest        ISSN： 1040-6387            Impact factor:   1.279


  71 in total

1.  The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments.

Authors:  Stephen A Bustin; Vladimir Benes; Jeremy A Garson; Jan Hellemans; Jim Huggett; Mikael Kubista; Reinhold Mueller; Tania Nolan; Michael W Pfaffl; Gregory L Shipley; Jo Vandesompele; Carl T Wittwer
Journal:  Clin Chem       Date:  2009-02-26       Impact factor: 8.327

2.  PCR: how to kill unwanted DNA.

Authors:  A M Prince; L Andrus
Journal:  Biotechniques       Date:  1992-03       Impact factor: 1.993

3.  Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction.

Authors:  K Mullis; F Faloona; S Scharf; R Saiki; G Horn; H Erlich
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1986

4.  Guidelines for Sanger sequencing and molecular assay monitoring.

Authors:  Beate M Crossley; Jianfa Bai; Amy Glaser; Roger Maes; Elizabeth Porter; Mary Lea Killian; Travis Clement; Kathy Toohey-Kurth
Journal:  J Vet Diagn Invest       Date:  2020-02-18       Impact factor: 1.279

5.  Laboratory diagnosis and transmissibility of bovine viral diarrhea virus from a bull with a persistent testicular infection.

Authors:  Benjamin W Newcomer; Kathy Toohey-Kurth; Yan Zhang; Bruce W Brodersen; M Shonda Marley; Kellye S Joiner; Yijing Zhang; Patricia K Galik; Kay P Riddell; M Daniel Givens
Journal:  Vet Microbiol       Date:  2014-03-02       Impact factor: 3.293

6.  Degradation of the Poliovirus 1 genome by chlorine dioxide.

Authors:  J Simonet; C Gantzer
Journal:  J Appl Microbiol       Date:  2006-04       Impact factor: 3.772

7.  Detection of Group B Streptococcus Directly from Collected ESwab Samples by Use of the BD Max GBS Assay.

Authors:  Suzane Silbert; Talita T Rocchetti; Alicia Gostnell; Carly Kubasek; Raymond Widen
Journal:  J Clin Microbiol       Date:  2016-04-06       Impact factor: 5.948

Review 8.  qPCR primer design revisited.

Authors:  Stephen Bustin; Jim Huggett
Journal:  Biomol Detect Quantif       Date:  2017-11-22

9.  Impact of storage conditions on the quality of nucleic acids in paraffin embedded tissues.

Authors:  Daniel Groelz; Christian Viertler; Daniela Pabst; Nadine Dettmann; Kurt Zatloukal
Journal:  PLoS One       Date:  2018-09-07       Impact factor: 3.240

Review 10.  Validation of laboratory tests for infectious diseases in wild mammals: review and recommendations.

Authors:  Beibei Jia; Axel Colling; David E Stallknecht; David Blehert; John Bingham; Beate Crossley; Debbie Eagles; Ian A Gardner
Journal:  J Vet Diagn Invest       Date:  2020-05-29       Impact factor: 1.279
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  2 in total

1.  Suggested guidelines for validation of real-time PCR assays in veterinary diagnostic laboratories.

Authors:  Kathy Toohey-Kurth; Monica M Reising; Rebecca L Tallmadge; Laura B Goodman; Jianfa Bai; Steven R Bolin; Janice C Pedersen; Mangkey A Bounpheng; Roman M Pogranichniy; Jane Christopher-Hennings; Mary Lea Killian; Donna M Mulrooney; Roger Maes; Shri Singh; Beate M Crossley
Journal:  J Vet Diagn Invest       Date:  2020-09-28       Impact factor: 1.279

2.  An Assessment of Diagnostic Assays and Sample Types in the Detection of an Attenuated Genotype 5 African Swine Fever Virus in European Pigs over a 3-Month Period.

Authors:  Karyn A Havas; Andrey E Gogin; Julia V Basalaeva; Irina P Sindryakova; Olga L Kolbasova; Ilya A Titov; Valentina M Lyska; Sergey Y Morgunov; Mikhail E Vlasov; Timofey A Sevskikh; Elena Y Pivova; Dmitry A Kudrjashov; Kent Doolittle; Silvia Zimmerman; Wendy Witbeck; Luis G Gimenez-Lirola; Joel Nerem; Gordon D Spronk; Jeffrey J Zimmerman; Alexey D Sereda
Journal:  Pathogens       Date:  2022-03-26
  2 in total

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