Literature DB >> 8027309

Inhibition of PCR-based assay for Bordetella pertussis by using calcium alginate fiber and aluminum shaft components of a nasopharyngeal swab.

R M Wadowsky1, S Laus, T Libert, S J States, G D Ehrlich.   

Abstract

A PCR-based assay for Bordetella pertussis was inhibited by using a calcium alginate fiber-tipped swab with an aluminum shaft but not by using a Dacron fiber-tipped swab with a plastic shaft. The calcium alginate fiber component inhibited the assay following storage for less than 1 min in a suspension of 10(3) CFU of B. pertussis per ml, whereas the aluminum shaft component required storage for at least 48 h in order to cause inhibition. We recommend the Dacron swab over the calcium alginate swab for collecting specimens for testing in PCR-based assays.

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Year:  1994        PMID： 8027309      PMCID： PMC267180          DOI： 10.1128/jcm.32.4.1054-1057.1994

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

1.  Calcium alginate swabs.

Authors:  M J Budzynski; M S Nelson; M T Boodée; T B McClelland
Journal:  J Forensic Sci       Date:  1992-05       Impact factor: 1.832

2.  Specific identification of Bordetella pertussis by the polymerase chain reaction.

Authors:  S Houard; C Hackel; A Herzog; A Bollen
Journal:  Res Microbiol       Date:  1989-09       Impact factor: 3.992

3.  Pertussis toxin gene: nucleotide sequence and genetic organization.

Authors:  C Locht; J M Keith
Journal:  Science       Date:  1986-06-06       Impact factor: 47.728

4.  Recovery of Bordetella pertussis from four kinds of swabs.

Authors:  J E Hoppe; A Weiss
Journal:  Eur J Clin Microbiol       Date:  1987-04       Impact factor: 3.267

5.  Comparison of specimen transport systems for Bordetella pertussis.

Authors:  J E Hoppe; S Wörz; K Botzenhart
Journal:  Eur J Clin Microbiol       Date:  1986-12       Impact factor: 3.267

6.  Analysis of a repetitive DNA sequence from Bordetella pertussis and its application to the diagnosis of pertussis using the polymerase chain reaction.

Authors:  E M Glare; J C Paton; R R Premier; A J Lawrence; I T Nisbet
Journal:  J Clin Microbiol       Date:  1990-09       Impact factor: 5.948

7.  Calcium alginate beads as a slow-release system for delivering angiogenic molecules in vivo and in vitro.

Authors:  E C Downs; N E Robertson; T L Riss; M L Plunkett
Journal:  J Cell Physiol       Date:  1992-08       Impact factor: 6.384

8.  Comparison of polymerase chain reaction with culture and enzyme immunoassay for diagnosis of pertussis.

Authors:  Q He; J Mertsola; H Soini; M Skurnik; O Ruuskanen; M K Viljanen
Journal:  J Clin Microbiol       Date:  1993-03       Impact factor: 5.948

9.  PCR detection and differentiation of Chlamydia pneumoniae, Chlamydia psittaci and Chlamydia trachomatis.

Authors:  S J Rasmussen; F P Douglas; P Timms
Journal:  Mol Cell Probes       Date:  1992-10       Impact factor: 2.365
  9 in total
  28 in total

Review 1.  Molecular diagnosis of Chlamydia pneumoniae infection.

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2.  Evaluation of Chlamydia pneumoniae and Mycoplasma pneumoniae as etiologic agents of persistent cough in adolescents and adults.

Authors:  Robert M Wadowsky; Elias A Castilla; Stella Laus; Anita Kozy; Robert W Atchison; Lawrence A Kingsley; Joel I Ward; David P Greenberg
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4.  Isolation of pneumococcal DNA from nasopharyngeal samples for real-time, quantitative PCR: comparison of three methods.

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Journal:  Mol Diagn       Date:  2003

5.  Impact of nasopharyngeal swab types on detection of Bordetella pertussis by PCR and culture.

Authors:  Joann L Cloud; Weston Hymas; Karen C Carroll
Journal:  J Clin Microbiol       Date:  2002-10       Impact factor: 5.948

Review 6.  Laboratory diagnosis of lower respiratory tract infections: controversy and conundrums.

Authors:  Karen C Carroll
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7.  Development and use of an internal positive control for detection of Bordetella pertussis by PCR.

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Journal:  J Clin Microbiol       Date:  2005-05       Impact factor: 5.948

8.  Prevalence and sequence variants of IS481 in Bordetella bronchiseptica: implications for IS481-based detection of Bordetella pertussis.

Authors:  Karen B Register; Gary N Sanden
Journal:  J Clin Microbiol       Date:  2006-10-25       Impact factor: 5.948

9.  Evaluation of PCR assays in presence of antibody to thermostable DNA polymerases for detection of microbial agents: avoiding false negative results for specimen containing low-titer agent.

Authors:  S K Poddar; M H Sawyer; J D Connor
Journal:  J Clin Lab Anal       Date:  1998       Impact factor: 2.352

10.  Immunomagnetic separation and solid-phase detection of Bordetella pertussis.

Authors:  M Stark; E Reizenstein; M Uhlén; J Lundeberg
Journal:  J Clin Microbiol       Date:  1996-04       Impact factor: 5.948
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