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Literature DB >> 9399539

Reproducibility of AMPLICOR enterovirus PCR test results.

Abstract

The reproducibility of AMPLICOR enterovirus PCR test results was determined with clinical samples of cerebrospinal fluid, serum, urine, and throat and rectal swabs. Among 608 samples from which duplicate aliquots were run simultaneously, only seven pairs gave discordant results. Among 104 samples from which duplicate aliquots were run in separate assays, no discordance was seen. Overall, the reproducibility of test kit results was 99% (705 of 712).

Entities: Species

Mesh：

Substances：
RNA, Viral

Year: 1997 PMID： 9399539 PMCID： PMC230167 DOI： 10.1128/jcm.35.12.3301-3302.1997

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

7 in total

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Authors: L P Nielsen; J F Modlin; H A Rotbart
Journal: Pediatr Infect Dis J Date: 1996-07 Impact factor: 2.129

Review 2. Nonpolio enteroviruses and the febrile young infant: epidemiologic, clinical and diagnostic aspects.

Authors: R Dagan
Journal: Pediatr Infect Dis J Date: 1996-01 Impact factor: 2.129

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Authors: H A Rotbart; A Ahmed; S Hickey; R Dagan; G H McCracken; R J Whitley; J F Modlin; M Cascino; J F O'Connell; M A Menegus; D Blum
Journal: Pediatr Infect Dis J Date: 1997-04 Impact factor: 2.129

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Journal: Nature Date: 1989-05-18 Impact factor: 49.962

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Authors: H A Rotbart; M H Sawyer; S Fast; C Lewinski; N Murphy; E F Keyser; J Spadoro; S Y Kao; M Loeffelholz
Journal: J Clin Microbiol Date: 1994-10 Impact factor: 5.948

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Authors: Y Schlesinger; M H Sawyer; G A Storch
Journal: Pediatrics Date: 1994-08 Impact factor: 7.124

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Authors: M J Abzug; M Loeffelholz; H A Rotbart
Journal: J Pediatr Date: 1995-03 Impact factor: 4.406

7 in total

1. Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens.

Authors: P P Young; R S Buller; G A Storch
Journal: J Clin Microbiol Date: 2000-11 Impact factor: 5.948

2. Comparison of the NucliSens Basic kit (Nucleic Acid Sequence-Based Amplification) and the Argene Biosoft Enterovirus Consensus Reverse Transcription-PCR assays for rapid detection of enterovirus RNA in clinical specimens.

Authors: Marie L Landry; Robin Garner; David Ferguson
Journal: J Clin Microbiol Date: 2003-11 Impact factor: 5.948

3. Correlation of cerebrospinal fluid (CSF) cell counts and elevated CSF protein levels with enterovirus reverse transcription-PCR results in pediatric and adult patients.

Authors: William S Mulford; Richard S Buller; Max Q Arens; Gregory A Storch
Journal: J Clin Microbiol Date: 2004-09 Impact factor: 5.948

4. Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked primer technology.

Authors: JiYoung Hong; Byunghak Kang; Ahyoun Kim; Seoyoun Hwang; Sunwha Lee; Jonghyen Kim; Hyun-Young Lee; Sang-Hyeon Kang; Doo-Sung Cheon
Journal: J Clin Microbiol Date: 2009-11-25 Impact factor: 5.948