Literature DB >> 2716852

Avoiding false positives with PCR.

S Kwok1, R Higuchi.   

Abstract

The exquisite sensitivity of the polymerase chain reaction means DNA contamination can ruin an entire experiment. Tidiness and adherence to a strict set of protocols can avoid disaster.

Mesh：

Year:  1989        PMID： 2716852     DOI： 10.1038/339237a0

Source DB:  PubMed          Journal:  Nature        ISSN： 0028-0836            Impact factor:   49.962


  736 in total

Review 1.  Molecular diagnosis of Chlamydia pneumoniae infection.

Authors:  J Boman; C A Gaydos; T C Quinn
Journal:  J Clin Microbiol       Date:  1999-12       Impact factor: 5.948

2.  Phylogenetic characterization of genotype 4 hepatitis C virus isolates from Argentina.

Authors:  V Alfonso; D Flichman; S Sookoian; V A Mbayed; R H Campos
Journal:  J Clin Microbiol       Date:  2001-05       Impact factor: 5.948

Review 3.  Demystified ... the polymerase chain reaction.

Authors:  K R Baumforth; P N Nelson; J E Digby; J D O'Neil; P G Murray
Journal:  Mol Pathol       Date:  1999-02

4.  Virus load and sequence variation in simian retrovirus type 2 infection.

Authors:  L L Rosenblum; R A Weiss; M O McClure
Journal:  J Virol       Date:  2000-04       Impact factor: 5.103

5.  Contamination and sensitivity issues with a real-time universal 16S rRNA PCR.

Authors:  C E Corless; M Guiver; R Borrow; V Edwards-Jones; E B Kaczmarski; A J Fox
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

6.  Quantitation of cell-free and cell-associated Kaposi's sarcoma-associated herpesvirus DNA by real-time PCR.

Authors:  I E White; T B Campbell
Journal:  J Clin Microbiol       Date:  2000-05       Impact factor: 5.948

7.  Diagnosis of pneumococcal pneumonia by polymerase chain reaction (PCR) in whole blood: a prospective clinical study.

Authors:  M L Lorente; M Falguera; A Nogués; A R González; M T Merino; M R Caballero
Journal:  Thorax       Date:  2000-02       Impact factor: 9.139

8.  Clinically applicable multiplex PCR for four middle ear pathogens.

Authors:  P H Hendolin; L Paulin; J Ylikoski
Journal:  J Clin Microbiol       Date:  2000-01       Impact factor: 5.948

9.  Contaminations occurring in fungal PCR assays.

Authors:  J Loeffler; H Hebart; R Bialek; L Hagmeyer; D Schmidt; F P Serey; M Hartmann; J Eucker; H Einsele
Journal:  J Clin Microbiol       Date:  1999-04       Impact factor: 5.948

Review 10.  Diagnosis of viral and chlamydial keratoconjunctivitis: which laboratory test?

Authors:  E M Elnifro; R J Cooper; P E Klapper; A S Bailey; A B Tullo
Journal:  Br J Ophthalmol       Date:  1999-05       Impact factor: 4.638
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