Literature DB >> 19940056

Enhanced detection of enteroviruses in clinical samples by reverse transcription-PCR using complementary locked primer technology.

JiYoung Hong1, Byunghak Kang, Ahyoun Kim, Seoyoun Hwang, Sunwha Lee, Jonghyen Kim, Hyun-Young Lee, Sang-Hyeon Kang, Doo-Sung Cheon.   

Abstract

To increase detection sensitivity, we modified primers using complementary locked primer (CLP) technology. The sensitivity of the reverse transcription-PCR (RT-PCR) with CLP-modified primers was 10- to 100-fold higher than that of RT-PCR without these primers. CLP-modified primers can increase sensitivity, providing a widely accessible method for molecular diagnosis.

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Year:  2009        PMID： 19940056      PMCID： PMC2815621          DOI： 10.1128/JCM.01790-09

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

1.  Rapid detection of enterovirus RNA in cerebrospinal fluid specimens with a novel single-tube real-time reverse transcription-PCR assay.

Authors:  W A Verstrepen; S Kuhn; M M Kockx; M E Van De Vyvere; A H Mertens
Journal:  J Clin Microbiol       Date:  2001-11       Impact factor: 5.948

2.  Evaluation of the roche AMPLICOR enterovirus PCR assay in the diagnosis of enteroviral central nervous system infections.

Authors:  K C Carroll; B Taggart; J Robison; C Byington; D Hillyard
Journal:  J Clin Virol       Date:  2000-12       Impact factor: 3.168

3.  Comparison of the NucliSens Basic kit (Nucleic Acid Sequence-Based Amplification) and the Argene Biosoft Enterovirus Consensus Reverse Transcription-PCR assays for rapid detection of enterovirus RNA in clinical specimens.

Authors:  Marie L Landry; Robin Garner; David Ferguson
Journal:  J Clin Microbiol       Date:  2003-11       Impact factor: 5.948

4.  General primer-mediated polymerase chain reaction for detection of enteroviruses: application for diagnostic routine and persistent infections.

Authors:  G J Zoll; W J Melchers; H Kopecka; G Jambroes; H J van der Poel; J M Galama
Journal:  J Clin Microbiol       Date:  1992-01       Impact factor: 5.948

5.  Diagnosis of enteroviral meningitis with the polymerase chain reaction.

Authors:  H A Rotbart
Journal:  J Pediatr       Date:  1990-07       Impact factor: 4.406

Review 6.  Reverse-transcription polymerase chain reaction detection of the enteroviruses.

Authors:  J R Romero
Journal:  Arch Pathol Lab Med       Date:  1999-12       Impact factor: 5.534

7.  Development and evaluation of a real-time RT-PCR assay on the LightCycler for the rapid detection of enterovirus in cerebrospinal fluid specimens.

Authors:  J Petitjean; A Vabret; J Dina; S Gouarin; F Freymuth
Journal:  J Clin Virol       Date:  2005-10-06       Impact factor: 3.168

8.  Modelling of silica diffusion experiments with 32Si in Boom Clay.

Authors:  Marc Aertsens; Pierre De Cannière; Hugo Moors
Journal:  J Contam Hydrol       Date:  2003-03       Impact factor: 3.188

9.  Aseptic meningitis in infants < 2 years of age: diagnosis and etiology.

Authors:  L E Berlin; M L Rorabaugh; F Heldrich; K Roberts; T Doran; J F Modlin
Journal:  J Infect Dis       Date:  1993-10       Impact factor: 5.226

10.  The impact of an enteroviral RT-PCR assay on the diagnosis of aseptic meningitis and patient management.

Authors:  K A Stellrecht; I Harding; A M Woron; M L Lepow; R A Venezia
Journal:  J Clin Virol       Date:  2002-07       Impact factor: 3.168
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  3 in total

1.  Development of a highly sensitive real-time one step RT-PCR combined complementary locked primer technology and conjugated minor groove binder probe.

Authors:  JiYoung Hong; Byunghak Kang; Ahyoun Kim; Seoyeon Hwang; Jinhee Ahn; Sunhwa Lee; Jonghyen Kim; Jae-Hak Park; Doo-Sung Cheon
Journal:  Virol J       Date:  2011-06-29       Impact factor: 4.099

2.  A comparative analysis of routine techniques: Reverse transcriptase polymerase chain reaction (RT-PCR) and five cell lines for detection of enteroviruses in stool specimens.

Authors:  F Abbasian; H Tabatabaie; M Sarijloo; S Shahmahmoodi; A Yousefi; T Saberbaghi; T Mokhtari Azad; R Nategh
Journal:  Iran J Microbiol       Date:  2011-06

3.  Sequence analysis of the first B5 subgenogroup strain of enterovirus 71 isolated in Korea.

Authors:  Yu Jung Won; Lae Hyung Kang; Ah Ra Lee; Bomina Paik; Hyun Kim; Sung Geun Lee; Seung Won Park; Seung Jin Hong; Soon Young Paik
Journal:  J Microbiol       Date:  2020-03-28       Impact factor: 3.422
  3 in total

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