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Literature DB >> 7814507

Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay.

H A Rotbart¹, M H Sawyer, S Fast, C Lewinski, N Murphy, E F Keyser, J Spadoro, S Y Kao, M Loeffelholz.

Abstract

A 5-h, user-friendly PCR assay for the diagnosis of enteroviral meningitis was developed. Reverse transcription and amplification were performed in a one-step reaction using rTth polymerase. Carryover contamination was prevented with dUTP and uracil N-glycosylate. Detection was performed colorimetrically on a microwell titer plate. Sensitivity, specificity, positive predictive value, and negative predictive value were 94.7, 97.4, 94.7, and 97.4%, respectively.

Entities: Chemical Disease

Mesh：

Year: 1994 PMID： 7814507 PMCID： PMC264111 DOI： 10.1128/jcm.32.10.2590-2592.1994

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

11 in total

1. General primer-mediated polymerase chain reaction for detection of enteroviruses: application for diagnostic routine and persistent infections.

Authors: G J Zoll; W J Melchers; H Kopecka; G Jambroes; H J van der Poel; J M Galama
Journal: J Clin Microbiol Date: 1992-01 Impact factor: 5.948

2. Diagnosis of enteroviral meningitis with the polymerase chain reaction.

Authors: H A Rotbart
Journal: J Pediatr Date: 1990-07 Impact factor: 4.406

3. Enzymatic RNA amplification of the enteroviruses.

Authors: H A Rotbart
Journal: J Clin Microbiol Date: 1990-03 Impact factor: 5.948

4. Temporal and geographic patterns of isolates of nonpolio enterovirus in the United States, 1970-1983.

Authors: R A Strikas; L J Anderson; R A Parker
Journal: J Infect Dis Date: 1986-02 Impact factor: 5.226

5. Detection and differentiation of picornaviruses in clinical samples following genomic amplification.

Authors: D M Olive; S Al-Mufti; W Al-Mulla; M A Khan; A Pasca; G Stanway; W Al-Nakib
Journal: J Gen Virol Date: 1990-09 Impact factor: 3.891

6. Diagnosis of enteroviral central nervous system infection by polymerase chain reaction during a large community outbreak.

Authors: M H Sawyer; D Holland; N Aintablian; J D Connor; E F Keyser; N J Waecker
Journal: Pediatr Infect Dis J Date: 1994-03 Impact factor: 2.129

7. Aseptic meningitis in infants < 2 years of age: diagnosis and etiology.

Authors: L E Berlin; M L Rorabaugh; F Heldrich; K Roberts; T Doran; J F Modlin
Journal: J Infect Dis Date: 1993-10 Impact factor: 5.226

8. Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction.

Authors: M J Loeffelholz; C A Lewinski; S R Silver; A P Purohit; S A Herman; D A Buonagurio; E A Dragon
Journal: J Clin Microbiol Date: 1992-11 Impact factor: 5.948

9. Detection of hepatitis C virus RNA by a combined reverse transcription-polymerase chain reaction assay.

Authors: K K Young; R M Resnick; T W Myers
Journal: J Clin Microbiol Date: 1993-04 Impact factor: 5.948

10. The clinical relevance of 'CSF viral culture'. A two-year experience with aseptic meningitis in Rochester, NY.

Authors: T Chonmaitree; M A Menegus; K R Powell
Journal: JAMA Date: 1982-04-02 Impact factor: 56.272

49 in total

1. Molecular detection and identification of an enterovirus during an outbreak of aseptic meningitis.

Authors: N Siafakas; A Georgopoulou; P Markoulatos; N Spyrou; G Stanway
Journal: J Clin Lab Anal Date: 2001 Impact factor: 2.352

2. Multicenter quality assessment of PCR methods for detection of enteroviruses.

Authors: P Muir; A Ras; P E Klapper; G M Cleator; K Korn; C Aepinus; A Fomsgaard; P Palmer; A Samuelsson; A Tenorio; B Weissbrich; A M van Loon
Journal: J Clin Microbiol Date: 1999-05 Impact factor: 5.948

3. Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens.

Authors: P P Young; R S Buller; G A Storch
Journal: J Clin Microbiol Date: 2000-11 Impact factor: 5.948

4. Rapid detection of enterovirus RNA in cerebrospinal fluid specimens with a novel single-tube real-time reverse transcription-PCR assay.

Authors: W A Verstrepen; S Kuhn; M M Kockx; M E Van De Vyvere; A H Mertens
Journal: J Clin Microbiol Date: 2001-11 Impact factor: 5.948

5. Multicenter proficiency testing of nucleic acid amplification methods for the detection of enteroviruses.

Authors: K E Van Vliet; P Muir; J M Echevarria; P E Klapper; G M Cleator; A M Van Loon
Journal: J Clin Microbiol Date: 2001-09 Impact factor: 5.948

6. Comparison of the NucliSens Basic kit (Nucleic Acid Sequence-Based Amplification) and the Argene Biosoft Enterovirus Consensus Reverse Transcription-PCR assays for rapid detection of enterovirus RNA in clinical specimens.

Authors: Marie L Landry; Robin Garner; David Ferguson
Journal: J Clin Microbiol Date: 2003-11 Impact factor: 5.948

7. New reverse transcription-PCR assay for rapid and sensitive detection of enterovirus genomes in cerebrospinal fluid specimens of patients with aseptic meningitis.

Authors: Jérôme Jacques; Jocelyne Carquin; Véronique Brodard; Hélène Moret; Dorine Lebrun; Maude Bouscambert; Jacques Motte; Gérard Rémy; Laurent Andréoletti
Journal: J Clin Microbiol Date: 2003-12 Impact factor: 5.948