Literature DB >> 11060104

Evaluation of a commercial DNA enzyme immunoassay for detection of enterovirus reverse transcription-PCR products amplified from cerebrospinal fluid specimens.

P P Young1, R S Buller, G A Storch.   

Abstract

We evaluated the DiaSorin DNA enzyme immunoassay (DEIA) kit for detection of enteroviral reverse transcription-PCR (RT-PCR) products amplified from cerebrospinal fluid. By use of an optical density of 0.05 as the absorbance cutoff, 35% of 198 specimens were PCR positive, whereas 16% were culture positive. DEIA was rapid and sensitive and can help implement enterovirus RT-PCR in clinical laboratories.

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Year:  2000        PMID： 11060104      PMCID： PMC87577     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  9 in total

1.  Multicenter quality assessment of PCR methods for detection of enteroviruses.

Authors:  P Muir; A Ras; P E Klapper; G M Cleator; K Korn; C Aepinus; A Fomsgaard; P Palmer; A Samuelsson; A Tenorio; B Weissbrich; A M van Loon
Journal:  J Clin Microbiol       Date:  1999-05       Impact factor: 5.948

2.  Clinical utility of the polymerase chain reaction for diagnosis of enteroviral meningitis in infancy.

Authors:  A Ahmed; F Brito; C Goto; S M Hickey; K D Olsen; M Trujillo; G H McCracken
Journal:  J Pediatr       Date:  1997-09       Impact factor: 4.406

3.  Enzymatic RNA amplification of the enteroviruses.

Authors:  H A Rotbart
Journal:  J Clin Microbiol       Date:  1990-03       Impact factor: 5.948

4.  Reproducibility of AMPLICOR enterovirus PCR test results.

Authors:  H A Rotbart
Journal:  J Clin Microbiol       Date:  1997-12       Impact factor: 5.948

5.  Diagnosis of enteroviral meningitis by using PCR with a colorimetric microwell detection assay.

Authors:  H A Rotbart; M H Sawyer; S Fast; C Lewinski; N Murphy; E F Keyser; J Spadoro; S Y Kao; M Loeffelholz
Journal:  J Clin Microbiol       Date:  1994-10       Impact factor: 5.948

6.  Detection of enteroviruses by spot hybridization.

Authors:  T Hyypiä; P Stålhandske; R Vainionpää; U Pettersson
Journal:  J Clin Microbiol       Date:  1984-03       Impact factor: 5.948

7.  Enteroviral meningitis in infancy: potential role for polymerase chain reaction in patient management.

Authors:  Y Schlesinger; M H Sawyer; G A Storch
Journal:  Pediatrics       Date:  1994-08       Impact factor: 7.124

8.  Evaluation of a commercially available reverse transcription-PCR assay for diagnosis of enteroviral infection in archival and prospectively collected cerebrospinal fluid specimens.

Authors:  F Pozo; I Casas; A Tenorio; G Trallero; J M Echevarria
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948

9.  Rapid diagnosis of enterovirus infection by magnetic bead extraction and polymerase chain reaction detection of enterovirus RNA in clinical specimens.

Authors:  P Muir; F Nicholson; M Jhetam; S Neogi; J E Banatvala
Journal:  J Clin Microbiol       Date:  1993-01       Impact factor: 5.948
  9 in total
  4 in total

1.  Detection of precytopathic effect of enteroviruses in clinical specimens by centrifugation-enhanced antigen detection.

Authors:  S M Lipson; K David; F Shaikh; L Qian
Journal:  J Clin Microbiol       Date:  2001-08       Impact factor: 5.948

Review 2.  Molecular approaches to detecting herpes simplex virus and enteroviruses in the central nervous system.

Authors:  Thomas W Smalling; Susan E Sefers; Haijing Li; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2002-07       Impact factor: 5.948

3.  Correlation of cerebrospinal fluid (CSF) cell counts and elevated CSF protein levels with enterovirus reverse transcription-PCR results in pediatric and adult patients.

Authors:  William S Mulford; Richard S Buller; Max Q Arens; Gregory A Storch
Journal:  J Clin Microbiol       Date:  2004-09       Impact factor: 5.948

4.  Comparison of coliforms and coliphages as tools for assessment of viral contamination in river water.

Authors:  S Skraber; B Gassilloud; C Gantzer
Journal:  Appl Environ Microbiol       Date:  2004-06       Impact factor: 4.792
  4 in total

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