Literature DB >> 9278501

Fluorescence-, isotope- or biotin-labeling of the 5 '-end of single-stranded DNA/RNA using T4 RNA ligase.

Y Kinoshita1, K Nishigaki, Y Husimi.   

Abstract

A rapid 5'-labeling method of single-stranded DNA/RNA was developed, which is based on the utilization of an adenylated intermediate in the reaction of T4 RNA ligase. This method is commonly useful for fluorescence-, isotope- or biotin-labeling of the 5'-ends of both oligo- and polynucleotides.

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Year:  1997        PMID： 9278501      PMCID： PMC146945          DOI： 10.1093/nar/25.18.3747

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  12 in total

1.  Protein biotinylation.

Authors:  E A Bayer; M Wilchek
Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

2.  Unexpectedly general replaceability of ATP in ATP-requiring enzymes.

Authors:  Y Kinoshita; K Nishigaki
Journal:  J Biochem       Date:  1997-07       Impact factor: 3.387

3.  Site specific labelling of sugar residues in oligoribonucleotides: reactions of aliphatic isocyanates with 2' amino groups.

Authors:  S T Sigurdson; F Eckstein
Journal:  Nucleic Acids Res       Date:  1996-08-15       Impact factor: 16.971

4.  Photocleavable biotin phosphoramidite for 5'-end-labeling, affinity purification and phosphorylation of synthetic oligonucleotides.

Authors:  J Olejnik; E Krzymańska-Olejnik; K J Rothschild
Journal:  Nucleic Acids Res       Date:  1996-01-15       Impact factor: 16.971

5.  Single-stranded DNA ligation by T4 RNA ligase for PCR cloning of 5'-noncoding fragments and coding sequence of a specific gene.

Authors:  X H Zhang; V L Chiang
Journal:  Nucleic Acids Res       Date:  1996-03-01       Impact factor: 16.971

6.  One-lane chemical sequencing of PCR amplified DNA: the use of terminal transferase and of the base analogue inosine.

Authors:  E Di Mauro; G Costanzo; R Negri
Journal:  Nucleic Acids Res       Date:  1994-09-11       Impact factor: 16.971

7.  Chemical and enzymatic biotin-labeling of oligodeoxyribonucleotides.

Authors:  T Kempe; W I Sundquist; F Chow; S L Hu
Journal:  Nucleic Acids Res       Date:  1985-01-11       Impact factor: 16.971

8.  Bacteriophage T4 RNA ligase. Reaction intermediates and interaction of substrates.

Authors:  A Sugino; T J Snoper; N R Cozzarelli
Journal:  J Biol Chem       Date:  1977-03-10       Impact factor: 5.157

9.  Incorporation of 1,N6-ethenoadenosine into the 3' terminus of tRNA using T4 RNA ligase. 2. Preparation and ribosome interaction of fluorescent Escherichia coli tRNAMetf.

Authors:  H Paulsen; W Wintermeyer
Journal:  Eur J Biochem       Date:  1984-01-02

10.  Single-step elongation of oligodeoxynucleotides using terminal deoxynucleotidyl transferase.

Authors:  H Schott; H Schrade
Journal:  Eur J Biochem       Date:  1984-09-17
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  15 in total

1.  Y-ligation: an efficient method for ligating single-stranded DNAs and RNAs with T4 RNA ligase.

Authors:  K Nishigaki; K Taguchi; Y Kinoshita; T Aita; Y Husimi
Journal:  Mol Divers       Date:  1998       Impact factor: 2.943

2.  Real-time monitoring of nucleic acid ligation in homogenous solutions using molecular beacons.

Authors:  Zhiwen Tang; Kemin Wang; Weihong Tan; Jun Li; Lingfeng Liu; Qiuping Guo; Xiangxian Meng; Changbei Ma; Shasheng Huang
Journal:  Nucleic Acids Res       Date:  2003-12-01       Impact factor: 16.971

3.  Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1.

Authors:  Thorarinn Blondal; Sigridur H Hjorleifsdottir; Olafur F Fridjonsson; Arnthor Aevarsson; Sigurlaug Skirnisdottir; Anna Gudny Hermannsdottir; Gudmundur O Hreggvidsson; Albert Vernon Smith; Jakob K Kristjansson
Journal:  Nucleic Acids Res       Date:  2003-12-15       Impact factor: 16.971

4.  Enzymes used in molecular biology: a useful guide.

Authors:  Laure Rittié; Bernard Perbal
Journal:  J Cell Commun Signal       Date:  2008-09-03       Impact factor: 5.782

5.  Oligoribonucleotide circularization by 'template-mediated' ligation with T4 RNA ligase: synthesis of circular hammerhead ribozymes.

Authors:  L Wang; D E Ruffner
Journal:  Nucleic Acids Res       Date:  1998-05-15       Impact factor: 16.971

Review 6.  Coming Together: RNAs and Proteins Assemble under the Single-Molecule Fluorescence Microscope.

Authors:  Ameya P Jalihal; Paul E Lund; Nils G Walter
Journal:  Cold Spring Harb Perspect Biol       Date:  2019-04-01       Impact factor: 10.005

7.  Two-step aminoacylation of tRNA without channeling in Archaea.

Authors:  Hari Bhaskaran; John J Perona
Journal:  J Mol Biol       Date:  2011-06-25       Impact factor: 5.469

Review 8.  Single molecule fluorescence approaches shed light on intracellular RNAs.

Authors:  Sethuramasundaram Pitchiaya; Laurie A Heinicke; Thomas C Custer; Nils G Walter
Journal:  Chem Rev       Date:  2014-01-08       Impact factor: 60.622

Review 9.  Fluorescent DNA-based enzyme sensors.

Authors:  Nan Dai; Eric T Kool
Journal:  Chem Soc Rev       Date:  2011-02-02       Impact factor: 54.564

10.  A two-stage mechanism of viral RNA compaction revealed by single molecule fluorescence.

Authors:  Alexander Borodavka; Roman Tuma; Peter G Stockley
Journal:  RNA Biol       Date:  2013-02-19       Impact factor: 4.652
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