Literature DB >> 9255945

Pulse schemes for the measurement of 3JC'C gamma and 3JNC gamma scalar couplings in 15N,13C uniformly labeled proteins.

R Konrat1, D R Muhandiram, N A Farrow, L E Kay.   

Abstract

Pulse sequences are presented for the measurement of 3JC'C gamma and 3JNC gamma scalar couplings for all C gamma containing residues in 15N,13C uniformly labeled proteins. The methods described are based on quantitative J correlation spectroscopy pioneered by Bax and co-workers [Bax et. al. (1994) Methods Enzymol., 239, 79-105]. The combination of 3JC'C gamma and 3JNC gamma scalar coupling constants allows the assignment of discrete rotameric states about the chi 1 torsion angle in cases where such states exist or, alternatively, facilitates the establishment of noncanonical chi 1 conformations or the presence of rotameric averaging. The methods are applied to a 1.5 mM sample of staphylococcal nuclease.

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Year:  1997        PMID： 9255945     DOI： 10.1023/a:1018354712430

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  12 in total

1.  Structures of larger proteins in solution: three- and four-dimensional heteronuclear NMR spectroscopy.

Authors:  G M Clore; A M Gronenborn
Journal:  Science       Date:  1991-06-07       Impact factor: 47.728

2.  The crystal structure of the ternary complex of staphylococcal nuclease, Ca2+, and the inhibitor pdTp, refined at 1.65 A.

Authors:  P J Loll; E E Lattman
Journal:  Proteins       Date:  1989

3.  NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors:  F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
Journal:  J Biomol NMR       Date:  1995-11       Impact factor: 2.835

Review 4.  Measurement of homo- and heteronuclear J couplings from quantitative J correlation.

Authors:  A Bax; G W Vuister; S Grzesiek; F Delaglio; A C Wang; R Tschudin; G Zhu
Journal:  Methods Enzymol       Date:  1994       Impact factor: 1.600

5.  Triple-resonance NOESY-based experiments with improved spectral resolution: applications to structural characterization of unfolded, partially folded and folded proteins.

Authors:  O Zhang; J D Forman-Kay; D Shortle; L E Kay
Journal:  J Biomol NMR       Date:  1997-02       Impact factor: 2.835

6.  Dynamics of methyl groups in proteins as studied by proton-detected 13C NMR spectroscopy. Application to the leucine residues of staphylococcal nuclease.

Authors:  L K Nicholson; L E Kay; D M Baldisseri; J Arango; P E Young; A Bax; D A Torchia
Journal:  Biochemistry       Date:  1992-06-16       Impact factor: 3.162

7.  Conformation of valine side chains in ribonuclease T1 determined by NMR studies of homonuclear and heteronuclear 3J coupling constants.

Authors:  Y Karimi-Nejad; J M Schmidt; H Rüterjans; H Schwalbe; C Greisinger
Journal:  Biochemistry       Date:  1994-05-10       Impact factor: 3.162

8.  Measurement of 15N-13C J couplings in staphylococcal nuclease.

Authors:  F Delaglio; D A Torchia; A Bax
Journal:  J Biomol NMR       Date:  1991-11       Impact factor: 2.835

9.  Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions.

Authors:  M Piotto; V Saudek; V Sklenár
Journal:  J Biomol NMR       Date:  1992-11       Impact factor: 2.835

10.  Heteronuclear relayed E.COSY applied to the determination of accurate 3J(HN,C') and 3J(H beta,C') coupling constants in desulfovibrio vulgaris flavodoxin.

Authors:  J M Schmidt; F Löhr; H Rüterjans
Journal:  J Biomol NMR       Date:  1996-03       Impact factor: 2.835
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  6 in total

1.  Simultaneous measurement of protein one-bond and two-bond nitrogen-carbon coupling constants using an internally referenced quantitative J-correlated [(15)N,(1)H]-TROSY-HNC experiment.

Authors:  Hans L J Wienk; Mitcheell M Martínez; Gary N Yalloway; Jürgen M Schmidt; Carlos Pérez; Heinz Rüterjans; Frank Löhr
Journal:  J Biomol NMR       Date:  2003-02       Impact factor: 2.835

2.  A New Experiment for the Measurement of nJ(C,P) Coupling Constants Including 3J(C4'i,Pi) and 3J(C4'i,Pi+1) in Oligonucleotides.

Authors:  C Richter; B Reif; K Wörner; S Quant; J P Marino; J W Engels; C Griesinger; H Schwalbe
Journal:  J Biomol NMR       Date:  1998-08       Impact factor: 2.835

3.  Accurate determination of leucine and valine side-chain conformations using U-[15N/13C/2H]/[1H-(methine/methyl)-Leu/Val] isotope labeling, NOE pattern recognition, and methine Cgamma-Hgamma/Cbeta-Hbeta residual dipolar couplings: application to the 34-kDa enzyme IIA(chitobiose).

Authors:  Chun Tang; Junji Iwahara; G Marius Clore
Journal:  J Biomol NMR       Date:  2005-10       Impact factor: 2.835

4.  Sensitivity enhanced NMR spectroscopy by quenching scalar coupling mediated relaxation: application to the direct observation of hydrogen bonds in 13C/15N-labeled proteins.

Authors:  A Liu; W Hu; S Qamar; A Majumdar
Journal:  J Biomol NMR       Date:  2000-05       Impact factor: 2.835

5.  Measuring the chi 1 torsion angle in protein by CH-CH cross-correlated relaxation: a new resolution-optimised experiment.

Authors:  Teresa Carlomagno; Wolfgang Bermel; Christian Griesinger
Journal:  J Biomol NMR       Date:  2003-10       Impact factor: 2.835

6.  100% complete assignment of non-labile (1)H, (13)C, and (15)N signals for calcium-loaded Calbindin D(9k) P43G.

Authors:  Nur Alia Oktaviani; Renee Otten; Klaas Dijkstra; Ruud M Scheek; Eva Thulin; Mikael Akke; Frans A A Mulder
Journal:  Biomol NMR Assign       Date:  2010-11-12       Impact factor: 0.746
  6 in total

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