Literature DB >> 21136326

A New Experiment for the Measurement of nJ(C,P) Coupling Constants Including 3J(C4'i,Pi) and 3J(C4'i,Pi+1) in Oligonucleotides.

C Richter1, B Reif, K Wörner, S Quant, J P Marino, J W Engels, C Griesinger, H Schwalbe.   

Abstract

A new experiment for the measurement of nJ(C,P) coupling constants along the phosphodiester backbone in RNA and DNA based on a quantitative-J HCP experiment is presented. In addition to coupling constants, in which a carbon atom couples to only one phosphorus atom, both the intraresidual 3J(C4'i,Pi) and the sequential 3J(C4'i,Pi+1) for the C4' resonances that couple to two phosphorus atoms can be obtained. Coupling constants obtained by this new method are compared to values obtained from the P-FIDS experiment. Together with 3J(H,P) coupling constants measured using the P-FIDS experiment, the backbone angles β and ∈ can be determined.

Entities:  

Year:  1998        PMID： 21136326     DOI： 10.1023/A:1008258226683

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  18 in total

1.  Chi 1 angle information from a simple two-dimensional NMR experiment that identifies trans 3JNC gamma couplings in isotopically enriched proteins.

Authors:  J S Hu; A Bax
Journal:  J Biomol NMR       Date:  1997-04       Impact factor: 2.835

2.  Pulse schemes for the measurement of 3JC'C gamma and 3JNC gamma scalar couplings in 15N,13C uniformly labeled proteins.

Authors:  R Konrat; D R Muhandiram; N A Farrow; L E Kay
Journal:  J Biomol NMR       Date:  1997-06       Impact factor: 2.835

3.  Determination of a complete set of coupling constants in 13C-labeled oligonucleotides.

Authors:  H Schwalbe; J P Marino; G C King; R Wechselberger; W Bermel; C Griesinger
Journal:  J Biomol NMR       Date:  1994-09       Impact factor: 2.835

4.  Efficient enzymatic synthesis of 13C,15N-labeled DNA for NMR studies.

Authors:  D E Smith; J Y Su; F M Jucker
Journal:  J Biomol NMR       Date:  1997-10       Impact factor: 2.835

5.  Preparation of isotopically labeled ribonucleotides for multidimensional NMR spectroscopy of RNA.

Authors:  R T Batey; M Inada; E Kujawinski; J D Puglisi; J R Williamson
Journal:  Nucleic Acids Res       Date:  1992-09-11       Impact factor: 16.971

6.  Three-dimensional heteronuclear NMR studies of RNA.

Authors:  E P Nikonowicz; A Pardi
Journal:  Nature       Date:  1992-01-09       Impact factor: 49.962

7.  Improved measurement of 13C, 31P J coupling constants in isotopically labeled RNA.

Authors:  P Legault; F M Jucker; A Pardi
Journal:  FEBS Lett       Date:  1995-04-03       Impact factor: 4.124

8.  NMR of enzymatically synthesized uniformly 13C15N-labeled DNA oligonucleotides.

Authors:  D P Zimmer; D M Crothers
Journal:  Proc Natl Acad Sci U S A       Date:  1995-04-11       Impact factor: 11.205

9.  Sequential backbone assignment of uniformly 13C-labeled RNAs by a two-dimensional P(CC)H-TOCSY triple resonance NMR experiment.

Authors:  S S Wijmenga; H A Heus; H A Leeuw; H Hoppe; M van der Graaf; C W Hilbers
Journal:  J Biomol NMR       Date:  1995-01       Impact factor: 2.835

10.  Carbon-13 NMR in conformational analysis of nucleic acid fragments. 2. A reparametrization of the Karplus equation for vicinal NMR coupling constants in CCOP and HCOP fragments.

Authors:  P P Lankhorst; C A Haasnoot; C Erkelens; C Altona
Journal:  J Biomol Struct Dyn       Date:  1984-06
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  3 in total

1.  A novel PH-cT-COSY methodology for measuring JPH coupling constants in unlabeled nucleic acids. application to HIV-2 TAR RNA.

Authors:  Teresa Carlomagno; Mirko Hennig; James R Williamson
Journal:  J Biomol NMR       Date:  2002-01       Impact factor: 2.835

2.  Conformational ensembles of RNA oligonucleotides from integrating NMR and molecular simulations.

Authors:  Sandro Bottaro; Giovanni Bussi; Scott D Kennedy; Douglas H Turner; Kresten Lindorff-Larsen
Journal:  Sci Adv       Date:  2018-05-18       Impact factor: 14.136

3.  High-resolution NMR structure of an RNA model system: the 14-mer cUUCGg tetraloop hairpin RNA.

Authors:  Senada Nozinovic; Boris Fürtig; Hendrik R A Jonker; Christian Richter; Harald Schwalbe
Journal:  Nucleic Acids Res       Date:  2009-11-11       Impact factor: 16.971
  3 in total

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