Literature DB >> 9177173

Transient aggregates in protein folding are easily mistaken for folding intermediates.

M Silow1, M Oliveberg.   

Abstract

It has been questioned recently whether populated intermediates are important for the protein folding process or are artefacts trapped in nonproductive pathways. We report here that the rapidly formed intermediate of the spliceosomal protein U1A is an off-pathway artefact caused by transient aggregation of denatured protein under native conditions. Transient aggregates are easily mistaken for structured monomers and could be a general problem in time-resolved folding studies.

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Year:  1997        PMID： 9177173      PMCID： PMC21005          DOI： 10.1073/pnas.94.12.6084

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  25 in total

1.  A kinetic study of the competition between renaturation and aggregation during the refolding of denatured-reduced egg white lysozyme.

Authors:  M E Goldberg; R Rudolph; R Jaenicke
Journal:  Biochemistry       Date:  1991-03-19       Impact factor: 3.162

2.  Folding of chymotrypsin inhibitor 2. 1. Evidence for a two-state transition.

Authors:  S E Jackson; A R Fersht
Journal:  Biochemistry       Date:  1991-10-29       Impact factor: 3.162

3.  Transient folding intermediates characterized by protein engineering.

Authors:  A Matouschek; J T Kellis; L Serrano; M Bycroft; A R Fersht
Journal:  Nature       Date:  1990-08-02       Impact factor: 49.962

Review 4.  Intermediates in the folding reactions of small proteins.

Authors:  P S Kim; R L Baldwin
Journal:  Annu Rev Biochem       Date:  1990       Impact factor: 23.643

5.  Crystal structure of the RNA-binding domain of the U1 small nuclear ribonucleoprotein A.

Authors:  K Nagai; C Oubridge; T H Jessen; J Li; P R Evans
Journal:  Nature       Date:  1990-12-06       Impact factor: 49.962

6.  Theory of cooperative transitions in protein molecules. I. Why denaturation of globular protein is a first-order phase transition.

Authors:  E I Shakhnovich; A V Finkelstein
Journal:  Biopolymers       Date:  1989-10       Impact factor: 2.505

7.  Structural characterization of folding intermediates in cytochrome c by H-exchange labelling and proton NMR.

Authors:  H Roder; G A Elöve; S W Englander
Journal:  Nature       Date:  1988-10-20       Impact factor: 49.962

8.  NMR evidence for an early framework intermediate on the folding pathway of ribonuclease A.

Authors:  J B Udgaonkar; R L Baldwin
Journal:  Nature       Date:  1988-10-20       Impact factor: 49.962

9.  Determination and analysis of urea and guanidine hydrochloride denaturation curves.

Authors:  C N Pace
Journal:  Methods Enzymol       Date:  1986       Impact factor: 1.600

Review 10.  Denatured states of proteins.

Authors:  K A Dill; D Shortle
Journal:  Annu Rev Biochem       Date:  1991       Impact factor: 23.643
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  50 in total

1.  Folding of beta-sandwich proteins: three-state transition of a fibronectin type III module.

Authors:  E Cota; J Clarke
Journal:  Protein Sci       Date:  2000-01       Impact factor: 6.725

2.  From snapshot to movie: phi analysis of protein folding transition states taken one step further.

Authors:  T Ternström; U Mayor; M Akke; M Oliveberg
Journal:  Proc Natl Acad Sci U S A       Date:  1999-12-21       Impact factor: 11.205

3.  Association of partially-folded intermediates of staphylococcal nuclease induces structure and stability.

Authors:  V N Uversky; A S Karnoup; R Khurana; D J Segel; S Doniach; A L Fink
Journal:  Protein Sci       Date:  1999-01       Impact factor: 6.725

4.  Reversible formation of on-pathway macroscopic aggregates during the folding of maltose binding protein.

Authors:  C Ganesh; F N Zaidi; J B Udgaonkar; R Varadarajan
Journal:  Protein Sci       Date:  2001-08       Impact factor: 6.725

5.  Exploring protein aggregation and self-propagation using lattice models: phase diagram and kinetics.

Authors:  R I Dima; D Thirumalai
Journal:  Protein Sci       Date:  2002-05       Impact factor: 6.725

6.  Observation of strange kinetics in protein folding.

Authors:  J Sabelko; J Ervin; M Gruebele
Journal:  Proc Natl Acad Sci U S A       Date:  1999-05-25       Impact factor: 11.205

7.  Solvent-amino acid interaction energies in three-dimensional-lattice Monte Carlo simulations of a model 27-mer protein: Folding thermodynamics and kinetics.

Authors:  Kai Leonhard; John M Prausnitz; Clayton J Radke
Journal:  Protein Sci       Date:  2004-02       Impact factor: 6.725

8.  Protein folding pathways and kinetics: molecular dynamics simulations of beta-strand motifs.

Authors:  Hyunbum Jang; Carol K Hall; Yaoqi Zhou
Journal:  Biophys J       Date:  2002-08       Impact factor: 4.033

9.  Expansion and compression of a protein folding intermediate by GroEL.

Authors:  Zong Lin; Hays S Rye
Journal:  Mol Cell       Date:  2004-10-08       Impact factor: 17.970

10.  GB1 is not a two-state folder: identification and characterization of an on-pathway intermediate.

Authors:  Angela Morrone; Rajanish Giri; Rudesh D Toofanny; Carlo Travaglini-Allocatelli; Maurizio Brunori; Valerie Daggett; Stefano Gianni
Journal:  Biophys J       Date:  2011-10-19       Impact factor: 4.033
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