Literature DB >> 8968935

Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test.

F Gamboa1, J M Manterola, B Viñado, L Matas, M Giménez, J Lonca, J R Manzano, C Rodrigo, P J Cardona, E Padilla, J Domínguez, V Ausina.   

Abstract

The Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test (AMTDT) was adapted for the detection of Mycobacterium tuberculosis complex in 224 nonrespiratory specimens from 188 patients. The sensitivity and specificity of the AMTDT for such specimens, after resolution of discrepant results, were 85.7 and 100%, respectively. Pretreatment of nonrespiratory specimens with sodium dodecyl (lauryl) sulfate is mandatory to obtain consistent and reproducible AMTDT results. The use of 500 microliters of decontaminated specimen improves the sensitivity of the test. Because the AMTDT detects stable rRNA from noncultivable bacilli, it is not useful for monitoring patients receiving treatment.

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Year:  1997        PMID： 8968935      PMCID： PMC229566          DOI： 10.1128/jcm.35.1.307-310.1997

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  26 in total

1.  Sensitivity and specificity of PCR for detection of Mycobacterium tuberculosis: a blind comparison study among seven laboratories.

Authors:  G T Noordhoek; A H Kolk; G Bjune; D Catty; J W Dale; P E Fine; P Godfrey-Faussett; S N Cho; T Shinnick; S B Svenson
Journal:  J Clin Microbiol       Date:  1994-02       Impact factor: 5.948

2.  A more reliable PCR for detection of Mycobacterium tuberculosis in clinical samples.

Authors:  L F Kox; D Rhienthong; A M Miranda; N Udomsantisuk; K Ellis; J van Leeuwen; S van Heusden; S Kuijper; A H Kolk
Journal:  J Clin Microbiol       Date:  1994-03       Impact factor: 5.948

3.  Diagnostic value of an amplification method (Gen-Probe) compared with that of culture for diagnosis of tuberculosis.

Authors:  F Vlaspolder; P Singer; C Roggeveen
Journal:  J Clin Microbiol       Date:  1995-10       Impact factor: 5.948

4.  Evaluation of Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test and PCR for direct detection of Mycobacterium tuberculosis in clinical specimens.

Authors:  N Miller; S G Hernandez; T J Cleary
Journal:  J Clin Microbiol       Date:  1994-02       Impact factor: 5.948

5.  Direct detection of Mycobacterium tuberculosis complex in respiratory specimens by a target-amplified test system.

Authors:  G E Pfyffer; P Kissling; R Wirth; R Weber
Journal:  J Clin Microbiol       Date:  1994-04       Impact factor: 5.948

6.  Evaluation of a commercial rRNA amplification assay for direct detection of Mycobacterium tuberculosis in processed sputum.

Authors:  M T La Rocco; A Wanger; H Ocera; E Macias
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1994-09       Impact factor: 3.267

7.  Evaluation of a commercial rRNA target amplification assay for detection of Mycobacterium tuberculosis complex in respiratory specimens.

Authors:  S Ehlers; M Pirmann; W Zaki; H Hahn
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1994-10       Impact factor: 3.267

8.  Use of Gen-Probe AccuProbes to identify Mycobacterium avium complex, Mycobacterium tuberculosis complex, Mycobacterium kansasii, and Mycobacterium gordonae directly from BACTEC TB broth cultures.

Authors:  B S Reisner; A M Gatson; G L Woods
Journal:  J Clin Microbiol       Date:  1994-12       Impact factor: 5.948

9.  Screening of respiratory tract specimens for the presence of Mycobacterium tuberculosis by using the Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test.

Authors:  T Bodmer; A Gurtner; K Schopfer; L Matter
Journal:  J Clin Microbiol       Date:  1994-06       Impact factor: 5.948

10.  Assessment of mycobacterial viability by RNA amplification.

Authors:  G M van der Vliet; P Schepers; R A Schukkink; B van Gemen; P R Klatser
Journal:  Antimicrob Agents Chemother       Date:  1994-09       Impact factor: 5.191
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  13 in total

1.  Evaluation of the BDProbeTec ET system for direct detection of Mycobacterium tuberculosis in pulmonary and extrapulmonary samples: a multicenter study.

Authors:  Gianna Mazzarelli; Laura Rindi; Paola Piccoli; Claudio Scarparo; Carlo Garzelli; Enrico Tortoli
Journal:  J Clin Microbiol       Date:  2003-04       Impact factor: 5.948

2.  Clinical evaluation of the automated COBAS AMPLICOR MTB assay for testing respiratory and nonrespiratory specimens.

Authors:  U Reischl; N Lehn; H Wolf; L Naumann
Journal:  J Clin Microbiol       Date:  1998-10       Impact factor: 5.948

3.  Rapid diagnosis of extrapulmonary tuberculosis by ligase chain reaction amplification.

Authors:  F Gamboa; J Dominguez; E Padilla; J M Manterola; E Gazapo; J Lonca; L Matas; A Hernandez; P J Cardona; V Ausina
Journal:  J Clin Microbiol       Date:  1998-05       Impact factor: 5.948

4.  Clinical evaluation of the enhanced Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test for rapid diagnosis of tuberculosis in prison inmates.

Authors:  J S Bergmann; G Yuoh; G Fish; G L Woods
Journal:  J Clin Microbiol       Date:  1999-05       Impact factor: 5.948

5.  Evaluation of Gen-Probe amplified mycobacterium tuberculosis direct test by using respiratory and nonrespiratory specimens in a tertiary care center laboratory.

Authors:  Cathal E O'Sullivan; David R Miller; Peggy S Schneider; Glenn D Roberts
Journal:  J Clin Microbiol       Date:  2002-05       Impact factor: 5.948

6.  Detection and identification of mycobacteria by amplification of RNA and DNA in pretreated blood and bone marrow aspirates by a simple lysis method.

Authors:  F Gamboa; J M Manterola; J Lonca; L Matas; B Viñado; M Giménez; P J Cardona; E Padilla; V Ausina
Journal:  J Clin Microbiol       Date:  1997-08       Impact factor: 5.948

7.  Detection of viable Mycobacterium tuberculosis by reverse transcriptase-strand displacement amplification of mRNA.

Authors:  T J Hellyer; L E DesJardin; L Teixeira; M D Perkins; M D Cave; K D Eisenach
Journal:  J Clin Microbiol       Date:  1999-03       Impact factor: 5.948

8.  Clinical evaluation of the BDProbeTec strand displacement amplification assay for rapid diagnosis of tuberculosis.

Authors:  J S Bergmann; G L Woods
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948

9.  Quantitative analysis of mRNA as a marker for viability of Mycobacterium tuberculosis.

Authors:  T J Hellyer; L E DesJardin; G L Hehman; M D Cave; K D Eisenach
Journal:  J Clin Microbiol       Date:  1999-02       Impact factor: 5.948

10.  Comparison of conventional bacteriology with nucleic acid amplification (amplified mycobacterium direct test) for diagnosis of tuberculous meningitis before and after inception of antituberculosis chemotherapy.

Authors:  Guy E Thwaites; Maxine Caws; Tran Thi Hong Chau; Nguyen Thi Dung; James I Campbell; Nguyen Hoan Phu; Tran Tinh Hien; Nicholas J White; Jeremy J Farrar
Journal:  J Clin Microbiol       Date:  2004-03       Impact factor: 5.948
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