Literature DB >> 7534230

Evaluation of a commercial rRNA target amplification assay for detection of Mycobacterium tuberculosis complex in respiratory specimens.

S Ehlers1, M Pirmann, W Zaki, H Hahn.   

Abstract

Seventy-one respiratory samples were analyzed in a new commercially available target rRNA amplification assay aimed at detecting Mycobacterium tuberculosis complex directly in patient specimens. The assay (Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test) correctly identified 26 of 27 samples positive for Mycobacterium tuberculosis and 43 of 44 samples negative for Mycobacterium tuberculosis in culture. When appropriate controls are performed, the assay also performs well with other specimens, such as tissue biopsies, lymph node or bone marrow aspirates and pleural and ascitic exudates.

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Year:  1994        PMID： 7534230     DOI： 10.1007/bf02111344

Source DB:  PubMed          Journal:  Eur J Clin Microbiol Infect Dis        ISSN： 0934-9723            Impact factor:   3.267


  15 in total

1.  Sputum digestion and decontamination with N-acetyl-L-cysteine-sodium hydroxide for culture of mycobacteria.

Authors:  G P KUBICA; W E DYE; M L COHN; G MIDDLEBROOK
Journal:  Am Rev Respir Dis       Date:  1963-05

2.  Assay formats involving acridinium-ester-labeled DNA probes.

Authors:  L J Arnold; P W Hammond; W A Wiese; N C Nelson
Journal:  Clin Chem       Date:  1989-08       Impact factor: 8.327

3.  Collaborative feasibility study of a biphasic system (Roche Septi-Chek AFB) for rapid detection and isolation of mycobacteria.

Authors:  H D Isenberg; R F D'Amato; L Heifets; P R Murray; M Scardamaglia; M C Jacobs; P Alperstein; A Niles
Journal:  J Clin Microbiol       Date:  1991-08       Impact factor: 5.948

4.  Specific detection of Mycobacterium tuberculosis complex strains by polymerase chain reaction.

Authors:  P W Hermans; A R Schuitema; D Van Soolingen; C P Verstynen; E M Bik; J E Thole; A H Kolk; J D van Embden
Journal:  J Clin Microbiol       Date:  1990-06       Impact factor: 5.948

5.  Polymerase chain reaction for detection of Mycobacterium tuberculosis in sputum.

Authors:  A B Andersen; S Thybo; P Godfrey-Faussett; N G Stoker
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1993-12       Impact factor: 3.267

6.  Sensitivity and specificity of PCR for detection of Mycobacterium tuberculosis: a blind comparison study among seven laboratories.

Authors:  G T Noordhoek; A H Kolk; G Bjune; D Catty; J W Dale; P E Fine; P Godfrey-Faussett; S N Cho; T Shinnick; S B Svenson
Journal:  J Clin Microbiol       Date:  1994-02       Impact factor: 5.948

7.  Automatable radiometric detection of growth of Mycobacterium tuberculosis in selective media.

Authors:  G Middlebrook; Z Reggiardo; W D Tigertt
Journal:  Am Rev Respir Dis       Date:  1977-06

8.  Detection of Mycobacterium tuberculosis in clinical specimens by polymerase chain reaction and Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test.

Authors:  C Abe; K Hirano; M Wada; Y Kazumi; M Takahashi; Y Fukasawa; T Yoshimura; C Miyagi; S Goto
Journal:  J Clin Microbiol       Date:  1993-12       Impact factor: 5.948

9.  Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by amplification of rRNA.

Authors:  V Jonas; M J Alden; J I Curry; K Kamisango; C A Knott; R Lankford; J M Wolfe; D F Moore
Journal:  J Clin Microbiol       Date:  1993-09       Impact factor: 5.948

10.  Direct detection of Mycobacterium tuberculosis complex in respiratory specimens by a target-amplified test system.

Authors:  G E Pfyffer; P Kissling; R Wirth; R Weber
Journal:  J Clin Microbiol       Date:  1994-04       Impact factor: 5.948
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  8 in total

1.  Rapid diagnosis of extrapulmonary tuberculosis by ligase chain reaction amplification.

Authors:  F Gamboa; J Dominguez; E Padilla; J M Manterola; E Gazapo; J Lonca; L Matas; A Hernandez; P J Cardona; V Ausina
Journal:  J Clin Microbiol       Date:  1998-05       Impact factor: 5.948

2.  Direct detection of Mycobacterium tuberculosis complex in nonrespiratory specimens by Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test.

Authors:  F Gamboa; J M Manterola; B Viñado; L Matas; M Giménez; J Lonca; J R Manzano; C Rodrigo; P J Cardona; E Padilla; J Domínguez; V Ausina
Journal:  J Clin Microbiol       Date:  1997-01       Impact factor: 5.948

3.  Diagnosis of extrapulmonary tuberculosis by Gen-Probe amplified Mycobacterium tuberculosis direct test.

Authors:  S Ehlers; R Ignatius; T Regnath; H Hahn
Journal:  J Clin Microbiol       Date:  1996-09       Impact factor: 5.948

4.  Genus level identification of mycobacteria from clinical specimens by using an easy-to-handle Mycobacterium-specific PCR assay.

Authors:  F Stauffer; H Haber; A Rieger; R Mutschlechner; P Hasenberger; V J Tevere; K K Young
Journal:  J Clin Microbiol       Date:  1998-03       Impact factor: 5.948

5.  Rapid diagnosis of extrapulmonary tuberculosis by PCR: impact of sample preparation and DNA extraction.

Authors:  S Honoré-Bouakline; J P Vincensini; V Giacuzzo; P H Lagrange; J L Herrmann
Journal:  J Clin Microbiol       Date:  2003-06       Impact factor: 5.948

6.  Diagnostic performance of amplified Mycobacterium tuberculosis direct test with cerebrospinal fluid, other nonrespiratory, and respiratory specimens.

Authors:  G E Pfyffer; P Kissling; E M Jahn; H M Welscher; M Salfinger; R Weber
Journal:  J Clin Microbiol       Date:  1996-04       Impact factor: 5.948

7.  Diagnostic value of an amplification method (Gen-Probe) compared with that of culture for diagnosis of tuberculosis.

Authors:  F Vlaspolder; P Singer; C Roggeveen
Journal:  J Clin Microbiol       Date:  1995-10       Impact factor: 5.948

8.  Commercial nucleic-acid amplification tests for diagnosis of pulmonary tuberculosis in respiratory specimens: meta-analysis and meta-regression.

Authors:  Daphne I Ling; Laura L Flores; Lee W Riley; Madhukar Pai
Journal:  PLoS One       Date:  2008-02-06       Impact factor: 3.240
  8 in total

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