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Literature DB >> 8955416

Inhibition of RecA-mediated cleavage in covalent dimers of UmuD.

Abstract

Disulfide-cross-linked UmuD2 derivatives were cleaved poorly upon incubation with activated RecA. Reducing the disulfide bonds prior to incubating the derivatives with RecA dramatically increased their extent of cleavage. These observations suggest that the UmuD monomer is a better substrate for the RecA-mediated cleavage reaction than the dimer.

Entities: Chemical Gene

Mesh：

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Year: 1996 PMID： 8955416 PMCID： PMC178647 DOI： 10.1128/jb.178.24.7304-7307.1996

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

27 in total

1. Dominant negative umuD mutations decreasing RecA-mediated cleavage suggest roles for intact UmuD in modulation of SOS mutagenesis.

Authors: J R Battista; T Ohta; T Nohmi; W Sun; G C Walker
Journal: Proc Natl Acad Sci U S A Date: 1990-09 Impact factor: 11.205

2. Dimerization of a specific DNA-binding protein on the DNA.

Authors: B Kim; J W Little
Journal: Science Date: 1992-01-10 Impact factor: 47.728

3. UmuC mutagenesis protein of Escherichia coli: purification and interaction with UmuD and UmuD'.

Authors: R Woodgate; M Rajagopalan; C Lu; H Echols
Journal: Proc Natl Acad Sci U S A Date: 1989-10 Impact factor: 11.205

4. The LexA repressor binds within the deep helical groove of the activated RecA filament.

Authors: X Yu; E H Egelman
Journal: J Mol Biol Date: 1993-05-05 Impact factor: 5.469

5. LexA and lambda Cl repressors as enzymes: specific cleavage in an intermolecular reaction.

Authors: B Kim; J W Little
Journal: Cell Date: 1993-06-18 Impact factor: 41.582

6. Interactions of Escherichia coli UmuD with activated RecA analyzed by cross-linking UmuD monocysteine derivatives.

Authors: M H Lee; G C Walker
Journal: J Bacteriol Date: 1996-12 Impact factor: 3.490

7. A monocysteine approach for probing the structure and interactions of the UmuD protein.

Authors: M H Lee; T Ohta; G C Walker
Journal: J Bacteriol Date: 1994-08 Impact factor: 3.490

8. In vitro analysis of mutant LexA proteins with an increased rate of specific cleavage.

Authors: K L Roland; M H Smith; J A Rupley; J W Little
Journal: J Mol Biol Date: 1992-11-20 Impact factor: 5.469

9. Autodigestion and RecA-dependent cleavage of Ind- mutant LexA proteins.

Authors: L L Lin; J W Little
Journal: J Mol Biol Date: 1989-12-05 Impact factor: 5.469

10. Structure of the UmuD' protein and its regulation in response to DNA damage.

Authors: T S Peat; E G Frank; J P McDonald; A S Levine; R Woodgate; W A Hendrickson
Journal: Nature Date: 1996-04-25 Impact factor: 49.962

5 in total

1. A model for a umuDC-dependent prokaryotic DNA damage checkpoint.

Authors: T Opperman; S Murli; B T Smith; G C Walker
Journal: Proc Natl Acad Sci U S A Date: 1999-08-03 Impact factor: 11.205

2. A role for the umuDC gene products of Escherichia coli in increasing resistance to DNA damage in stationary phase by inhibiting the transition to exponential growth.

Authors: S Murli; T Opperman; B T Smith; G C Walker
Journal: J Bacteriol Date: 2000-02 Impact factor: 3.490