Literature DB >> 8785501

Sequential assignments in uniformly 13C- and 15N-labelled RNAs: the HC(N,P) and HC(N,P)-CCH-TOCSY experiments.

R Ramachandran1, C Sich, M Grüne, V Soskic, L R Brown.   

Abstract

An approach for the simultaneous acquisition of HCN and HCP as well as HCN-CCH-TOCSY and HCP-CCH-TOCSY triple resonance data sets for 13C-/15N-labelled RNAs is presented. The new HCN-CCH-TOCSY scheme unambiguously links all sugar resonances to the base nitrogen. In addition, simultaneous acquisition of HCN-CCH-TOCSY and HCP-CCH-TOCSY data sets provides sequential and base-type information in a single experiment, thereby saving data acquisition time as well as providing complementary data sets that are useful in clarifying ambiguous assignments. Virtually complete sequence-specific phosphate-ribose 1H, 31P, and base 15N1,9, assignments as well as partial 13C assignments could be obtained in a single experiment for a 0.5-mM sample of a 19-mer ribonucleotide.

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Year:  1996        PMID： 8785501     DOI： 10.1007/bf00202042

Source DB:  PubMed          Journal:  J Biomol NMR        ISSN： 0925-2738            Impact factor:   2.835


  12 in total

Review 1.  RNA structure and NMR spectroscopy.

Authors:  G Varani; I Tinoco
Journal:  Q Rev Biophys       Date:  1991-11       Impact factor: 5.318

2.  Synthesis and purification of large amounts of RNA oligonucleotides.

Authors:  J R Wyatt; M Chastain; J D Puglisi
Journal:  Biotechniques       Date:  1991-12       Impact factor: 1.993

3.  Preparation of 13C and 15N labelled RNAs for heteronuclear multi-dimensional NMR studies.

Authors:  E P Nikonowicz; A Sirr; P Legault; F M Jucker; L M Baer; A Pardi
Journal:  Nucleic Acids Res       Date:  1992-09-11       Impact factor: 16.971

4.  Preparation of isotopically labeled ribonucleotides for multidimensional NMR spectroscopy of RNA.

Authors:  R T Batey; M Inada; E Kujawinski; J D Puglisi; J R Williamson
Journal:  Nucleic Acids Res       Date:  1992-09-11       Impact factor: 16.971

5.  Two- and three-dimensional HCN experiments for correlating base and sugar resonances in 15N,13C-labeled RNA oligonucleotides.

Authors:  V Sklenár; R D Peterson; M R Rejante; J Feigon
Journal:  J Biomol NMR       Date:  1993-11       Impact factor: 2.835

6.  The nature of preferred hairpin structures in 16S-like rRNA variable regions.

Authors:  J Wolters
Journal:  Nucleic Acids Res       Date:  1992-04-25       Impact factor: 16.971

7.  Sequential backbone assignment in 13C-labeled DNA by the 1H, 13C, 31P triple-resonance experiment, HCP-CCH-COSY.

Authors:  S Tate; A Ono; M Kainosho
Journal:  J Magn Reson B       Date:  1995-01

8.  Unambiguous through-bond sugar-to-base correlations for purines in 13C,15N-labeled nucleic acids: the HsCsNb,HsCs(N)bCb, and HbNbCb experiments.

Authors:  B T Farmer; L Müller; E P Nikonowicz; A Pardi
Journal:  J Biomol NMR       Date:  1994-01       Impact factor: 2.835

9.  Sequential backbone assignment of uniformly 13C-labeled RNAs by a two-dimensional P(CC)H-TOCSY triple resonance NMR experiment.

Authors:  S S Wijmenga; H A Heus; H A Leeuw; H Hoppe; M van der Graaf; C W Hilbers
Journal:  J Biomol NMR       Date:  1995-01       Impact factor: 2.835

10.  Correlation of nucleotide base and sugar protons in a 15N-labeled HIV-1 RNA oligonucleotide by 1H-15N HSQC experiments.

Authors:  V Sklenár; R D Peterson; M R Rejante; J Feigon
Journal:  J Biomol NMR       Date:  1994-01       Impact factor: 2.835
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Authors:  J Leppert; B Heise; R Ramachandran
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3.  Transverse relaxation optimized triple-resonance NMR experiments for nucleic acids.

Authors:  R Fiala; J Czernek; V Sklenár
Journal:  J Biomol NMR       Date:  2000-04       Impact factor: 2.835

4.  Multiple-quantum HCN-CCH-TOCSY experiment for 13C/15N labeled RNA oligonucleotides.

Authors:  W Hu; L Jiang
Journal:  J Biomol NMR       Date:  1999-12       Impact factor: 2.835

5.  Automated NMR resonance assignment strategy for RNA via the phosphodiester backbone based on high-dimensional through-bond APSY experiments.

Authors:  Barbara Krähenbühl; Issam El Bakkali; Elena Schmidt; Peter Güntert; Gerhard Wider
Journal:  J Biomol NMR       Date:  2014-04-27       Impact factor: 2.835

6.  A TROSY relayed HCCH-COSY experiment for correlating adenine H2/H8 resonances in uniformly 13C-labeled RNA molecules.

Authors:  B Simon; K Zanier; M Sattler
Journal:  J Biomol NMR       Date:  2001-06       Impact factor: 2.835

7.  GFT projection NMR for efficient (1)H/ (13)C sugar spin system identification in nucleic acids.

Authors:  Hanudatta S Atreya; Bharathwaj Sathyamoorthy; Garima Jaipuria; Victor Beaumont; Gabriele Varani; Thomas Szyperski
Journal:  J Biomol NMR       Date:  2012-11-29       Impact factor: 2.835

8.  Sequential acquisition of multi-dimensional heteronuclear chemical shift correlation spectra with ¹H detection.

Authors:  Peter Bellstedt; Yvonne Ihle; Christoph Wiedemann; Anika Kirschstein; Christian Herbst; Matthias Görlach; Ramadurai Ramachandran
Journal:  Sci Rep       Date:  2014-03-27       Impact factor: 4.379

9.  C4'/H4' selective, non-uniformly sampled 4D HC(P)CH experiment for sequential assignments of (13)C-labeled RNAs.

Authors:  Saurabh Saxena; Jan Stanek; Mirko Cevec; Janez Plavec; Wiktor Koźmiński
Journal:  J Biomol NMR       Date:  2014-09-10       Impact factor: 2.835
  9 in total

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