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Literature DB >> 23192291

GFT projection NMR for efficient (1)H/ (13)C sugar spin system identification in nucleic acids.

Hanudatta S Atreya¹, Bharathwaj Sathyamoorthy, Garima Jaipuria, Victor Beaumont, Gabriele Varani, Thomas Szyperski.

Abstract

A newly implemented G-matrix Fourier transform (GFT) (4,3)D HC(C)CH experiment is presented in conjunction with (4,3)D HCCH to efficiently identify (1)H/(13)C sugar spin systems in (13)C labeled nucleic acids. This experiment enables rapid collection of highly resolved relay 4D HC(C)CH spectral information, that is, shift correlations of (13)C-(1)H groups separated by two carbon bonds. For RNA, (4,3)D HC(C)CH takes advantage of the comparably favorable 1'- and 3'-CH signal dispersion for complete spin system identification including 5'-CH. The (4,3)D HC(C)CH/HCCH based strategy is exemplified for the 30-nucleotide 3'-untranslated region of the pre-mRNA of human U1A protein.

Entities: Chemical Disease Gene Species

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Year: 2012 PMID： 23192291 DOI： 10.1007/s10858-012-9687-5

Source DB: PubMed Journal: J Biomol NMR ISSN： 0925-2738 Impact factor: 2.835

31 in total

1. The 2D [31P] spin-echo-difference constant-time [13C, 1H]-HMQC experiment for simultaneous determination of 3J(H3'P) and 3J(C4'P) in 13C-labeled nucleic acids and their protein complexes.

Authors: T Szyperski; C Fernández; A Ono; K Wüthrich; M Kainosho
Journal: J Magn Reson Date: 1999-10 Impact factor: 2.229

2. Probing structure and functional dynamics of (large) proteins with aromatic rings: L-GFT-TROSY (4,3)D HCCH NMR spectroscopy.

Authors: Alexander Eletsky; Hanudatta S Atreya; Gaohua Liu; Thomas Szyperski
Journal: J Am Chem Soc Date: 2005-10-26 Impact factor: 15.419

Review 3. RNA structure and NMR spectroscopy.

Authors: G Varani; I Tinoco
Journal: Q Rev Biophys Date: 1991-11 Impact factor: 5.318

4. The program XEASY for computer-supported NMR spectral analysis of biological macromolecules.

Authors: C Bartels; T H Xia; M Billeter; P Güntert; K Wüthrich
Journal: J Biomol NMR Date: 1995-07 Impact factor: 2.835

5. Resonance assignment of proteins with high shift degeneracy based on 5D spectral information encoded in G2FT NMR experiments.

Authors: Hanudatta S Atreya; Alexander Eletsky; Thomas Szyperski
Journal: J Am Chem Soc Date: 2005-04-06 Impact factor: 15.419

6. NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

Authors: F Delaglio; S Grzesiek; G W Vuister; G Zhu; J Pfeifer; A Bax
Journal: J Biomol NMR Date: 1995-11 Impact factor: 2.835

7. Conformational changes of the BS2 operator DNA upon complex formation with the Antennapedia homeodomain studied by NMR with 13C/15N-labeled DNA.

Authors: C Fernández; T Szyperski; M Billeter; A Ono; H Iwai; M Kainosho; K Wüthrich
Journal: J Mol Biol Date: 1999-09-24 Impact factor: 5.469

8. Novel three-dimensional 1H-13C-31P triple resonance experiments for sequential backbone correlations in nucleic acids.

Authors: G Varani; F Aboul-ela; F Allain; C C Gubser
Journal: J Biomol NMR Date: 1995-04 Impact factor: 2.835

GFT projection NMR for efficient (1)H/ (13)C sugar spin system identification in nucleic acids.

1. The 2D [31P] spin-echo-difference constant-time [13C, 1H]-HMQC experiment for simultaneous determination of 3J(H3'P) and 3J(C4'P) in 13C-labeled nucleic acids and their protein complexes.

2. Probing structure and functional dynamics of (large) proteins with aromatic rings: L-GFT-TROSY (4,3)D HCCH NMR spectroscopy.

Review 3. RNA structure and NMR spectroscopy.

4. The program XEASY for computer-supported NMR spectral analysis of biological macromolecules.

5. Resonance assignment of proteins with high shift degeneracy based on 5D spectral information encoded in G2FT NMR experiments.

6. NMRPipe: a multidimensional spectral processing system based on UNIX pipes.

7. Conformational changes of the BS2 operator DNA upon complex formation with the Antennapedia homeodomain studied by NMR with 13C/15N-labeled DNA.

8. Novel three-dimensional 1H-13C-31P triple resonance experiments for sequential backbone correlations in nucleic acids.

9. Sequential assignments in uniformly 13C- and 15N-labelled RNAs: the HC(N,P) and HC(N,P)-CCH-TOCSY experiments.

10. Improved measurement of 13C, 31P J coupling constants in isotopically labeled RNA.

1. Development and application of aromatic [(13)C, (1)H] SOFAST-HMQC NMR experiment for nucleic acids.

2. Spatially selective heteronuclear multiple-quantum coherence spectroscopy for biomolecular NMR studies.

3. Strategy for automated NMR resonance assignment of RNA: application to 48-nucleotide K10.