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Literature DB >> 866195

Salt-induced structural changes in nucleosomes.

Abstract

Nucleosomes and oligonucleosomes were prepared by digestion of human placental nuclei with staphlococcal nuclease and fractionated by gel filtration chromatography. The effect of increasing salt on the structure of nucleosomes was examined in the presence and absence of 10 mM MgCl2. Nucleosomes and oligonucleosomes are insoluble over a broad range of salt concentration. Nucleosomes are insoluble in larger than or equal to 120 mM (NH4)2SO4 containing 10 mM MgCl2 allowing analyses of changes in nucleosomal DNA by C.D. spectroscopy. Nucleosomes are insoluble in less than or equal to 120 mM (NH4)2SO4 containing 10 mM MgCl2 as demonstrated by turbidity measurements. We conclude that the insolubility of nucleosomes accompanies salt-induced structural changes possibly due to individual particle condensation. As the salt concentration is increased the nucleosomes condense and then relax at higher salt concentrations.

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Year: 1977 PMID： 866195 PMCID： PMC342489 DOI： 10.1093/nar/4.4.853

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

37 in total

Salt-induced structural changes in nucleosomes.

1. The structure of the globin genes in chromatin.

2. Thermal denaturation profiles and the structure of chromatin.

3. Low-angle neutron scattering from chromatin subunit particles.

4. Chromatin and nucleosome structure.

5. The reversible denaturation of deoxyribonucleic acid-dependent ribonucleic acid polymerase of Escherichia coli.

6. Spheroid chromatin units (v bodies).

7. Structure of chromatin.

8. Biochemical evidence of variability in the DNA repeat length in the chromatin of higher eukaryotes.

9. RNA chain elongation on a chromatin template.

10. Template properties of liver chromatin.

1. The sv40 transcription complex. II. Non-dissociation of protein from SV40 chromatin during transcription.

2. The sv40 transcription complex. I. Effect of viral chromatin proteins on endogenous RNA polymerase activity.

3. Conformational changes of the chromatin subunit.

4. Chromosome-bound mitotic factors: release by endonucleases.

5. Transcription of nucleosomes from human chromatin.

6. Chromosomal localization of the human placental lactogen-growth hormone gene cluster to 17q22-24.

7. Responses of mammalian metaphase chromosomes to endonuclease digestion.

8. Isolation and characterization of a spacerless dinucleosome from H1-deleted chromatin.

9. Use of RNA polymerase as an enzymatic probe of nucleosomal structure.