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Literature DB >> 600791

Isolation and characterization of a spacerless dinucleosome from H1-deleted chromatin.

Abstract

Calf thymus chromatin, depleted in histone H1, was digested with micrococcal nuclease and fractionated by column chromatography. 140 base pair nucleosome core particles were isolated along with an unusual particle containing 2 histone octamers and 240 base pairs of DNA. Evidence is presented that the spacer DNA region is absent from these modified dinucleosomes, which appear as stable products of the digestion process. The physical properties of both particles are presented along with brief speculation on their possible origin and function.

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Year: 1977 PMID： 600791 PMCID： PMC343227 DOI： 10.1093/nar/4.12.4077

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

34 in total

1. Chromatin nu bodies: isolation, subfractionation and physical characterization.

Authors: A L Olins; R D Carlson; E B Wright; D E Olins
Journal: Nucleic Acids Res Date: 1976-12 Impact factor: 16.971

2. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

3. The DNA repeat lengths in chromatins from sea urchin sperm and gastrule cells are markedly different.

Authors: C Spadafora; M Bellard; J L Compton; P Chambon
Journal: FEBS Lett Date: 1976-10-15 Impact factor: 4.124

4. Quantitative analysis of the digestion of yeast chromatin by staphylococcal nuclease.

Authors: D Lohr; R T Kovacic; K E Van Holde
Journal: Biochemistry Date: 1977-02-08 Impact factor: 3.162

5. Comparative subunit structure of HeLa, yeast, and chicken erythrocyte chromatin.

Authors: D Lohr; J Corden; K Tatchell; R T Kovacic; K E Van Holde
Journal: Proc Natl Acad Sci U S A Date: 1977-01 Impact factor: 11.205

6. Action of micrococcal nuclease on chromatin and the location of histone H1.

Authors: M Noll; R D Kornberg
Journal: J Mol Biol Date: 1977-01-25 Impact factor: 5.469

7. Proteolytic digestion of histones indicates that the nucleosomes of nuclei and of sheared chromatin are similar.

Authors: D B Marks; B J Keller
Journal: Biochem Biophys Res Commun Date: 1977-01-10 Impact factor: 3.575

8. Nuclease digestion of reconstituted chromatin.

Authors: M Yaneva; B Tasheva; G Dessev
Journal: FEBS Lett Date: 1976-11 Impact factor: 4.124

9. Salt-induced structural changes in nucleosomes.

Authors: C G Sahasrabuddhe; G F Saunders
Journal: Nucleic Acids Res Date: 1977-04 Impact factor: 16.971

10. Studies of ColE1-plasmid DNA and its interactions with histones: sedimentation velocity studies of monodisperse complexes reconstituted with calf-thymus histones.

Authors: G Voordouw; D Kalif; H Eisenberg
Journal: Nucleic Acids Res Date: 1977 Impact factor: 16.971

17 in total

Isolation and characterization of a spacerless dinucleosome from H1-deleted chromatin.

1. Chromatin nu bodies: isolation, subfractionation and physical characterization.

2. Protein measurement with the Folin phenol reagent.

3. The DNA repeat lengths in chromatins from sea urchin sperm and gastrule cells are markedly different.

4. Quantitative analysis of the digestion of yeast chromatin by staphylococcal nuclease.

5. Comparative subunit structure of HeLa, yeast, and chicken erythrocyte chromatin.

6. Action of micrococcal nuclease on chromatin and the location of histone H1.

7. Proteolytic digestion of histones indicates that the nucleosomes of nuclei and of sheared chromatin are similar.

8. Nuclease digestion of reconstituted chromatin.

9. Salt-induced structural changes in nucleosomes.

10. Studies of ColE1-plasmid DNA and its interactions with histones: sedimentation velocity studies of monodisperse complexes reconstituted with calf-thymus histones.

1. Specific chemical labeling of DNA fragments.

2. Driving proteins off DNA using applied tension.

3. Compact oligomers and nucleosome phasing.

4. 31P NMR studies of the solution structure and dynamics of nucleosomes and DNA.

5. Circular dichroism analysis of mononucleosome DNA conformation.

Review 6. Chromatin replication, reconstitution and assembly.

7. Effect of DNA length on the nucleosome low salt transition.

8. Reduced repeat length of nascent nucleosomal DNA is generated by replicating chromatin in vivo.

9. Solution structural studies of the A and Z forms of DNA.

10. In vitro core particle and nucleosome assembly at physiological ionic strength.