Literature DB >> 8464924

Molecular cloning and expression of cDNA for mammalian translation initiation factor 5.

K Das1, J Chevesich, U Maitra.   

Abstract

Eukaryotic translation initiation factor 5 (eIF-5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.AUG.Met-tRNAf-eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the formation of a functional 80S initiation complex. A rat cDNA that encodes eIF-5 has been isolated and expressed in Escherichia coli to yield a catalytically active eIF-5 protein. The 3.55-kb cDNA encodes a protein of 429 amino acids (calculated M(r) 48,926) with properties that are similar to eIF-5 isolated from rabbit reticulocyte lysates. The deduced amino acid sequence of eIF-5 contains sequence motifs characteristic of proteins of the GTPase superfamily.

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Year:  1993        PMID： 8464924      PMCID： PMC46236          DOI： 10.1073/pnas.90.7.3058

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  27 in total

1.  Characterization of eukaryotic initiation factor 5 from rabbit reticulocytes. Evidence that the initiation factor is a monomeric protein of Mr of about 58,000-62,000.

Authors:  P Raychaudhuri; J Chevesich; S Ghosh; U Maitra
Journal:  J Biol Chem       Date:  1987-10-15       Impact factor: 5.157

2.  Identification of two messenger RNA cap binding proteins in wheat germ. Evidence that the 28-kDa subunit of eIF-4B and the 26-kDa subunit of eIF-4F are antigenically distinct polypeptides.

Authors:  K S Browning; S R Lax; J M Ravel
Journal:  J Biol Chem       Date:  1987-08-15       Impact factor: 5.157

3.  Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

Authors:  H Schägger; G von Jagow
Journal:  Anal Biochem       Date:  1987-11-01       Impact factor: 3.365

4.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Authors:  P Chomczynski; N Sacchi
Journal:  Anal Biochem       Date:  1987-04       Impact factor: 3.365

5.  Lambda gt 11: gene isolation with antibody probes and other applications.

Authors:  M Snyder; S Elledge; D Sweetser; R A Young; R W Davis
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

6.  Rat preprocarboxypeptidase H. Cloning, characterization, and sequence of the cDNA and regulation of the mRNA by corticotropin-releasing factor.

Authors:  C Rodríguez; K A Brayton; M Brownstein; J E Dixon
Journal:  J Biol Chem       Date:  1989-04-05       Impact factor: 5.157

7.  A comprehensive set of sequence analysis programs for the VAX.

Authors:  J Devereux; P Haeberli; O Smithies
Journal:  Nucleic Acids Res       Date:  1984-01-11       Impact factor: 16.971

8.  Brain-specific genes have identifier sequences in their introns.

Authors:  R J Milner; F E Bloom; C Lai; R A Lerner; J G Sutcliffe
Journal:  Proc Natl Acad Sci U S A       Date:  1984-02       Impact factor: 11.205

9.  Further characterization of eukaryotic initiation factor 5 from rabbit reticulocytes. Immunochemical characterization and phosphorylation by casein kinase II.

Authors:  S Ghosh; J Chevesich; U Maitra
Journal:  J Biol Chem       Date:  1989-03-25       Impact factor: 5.157

10.  Formation and release of eukaryotic initiation factor 2 X GDP complex during eukaryotic ribosomal polypeptide chain initiation complex formation.

Authors:  P Raychaudhuri; A Chaudhuri; U Maitra
Journal:  J Biol Chem       Date:  1985-02-25       Impact factor: 5.157
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  9 in total

1.  Mutational analysis of mammalian translation initiation factor 5 (eIF5): role of interaction between the beta subunit of eIF2 and eIF5 in eIF5 function in vitro and in vivo.

Authors:  S Das; U Maitra
Journal:  Mol Cell Biol       Date:  2000-06       Impact factor: 4.272

2.  Archaeal translation initiation revisited: the initiation factor 2 and eukaryotic initiation factor 2B alpha-beta-delta subunit families.

Authors:  N C Kyrpides; C R Woese
Journal:  Proc Natl Acad Sci U S A       Date:  1998-03-31       Impact factor: 11.205

Review 3.  The plant translational apparatus.

Authors:  K S Browning
Journal:  Plant Mol Biol       Date:  1996-10       Impact factor: 4.076

4.  GTP hydrolysis controls stringent selection of the AUG start codon during translation initiation in Saccharomyces cerevisiae.

Authors:  H K Huang; H Yoon; E M Hannig; T F Donahue
Journal:  Genes Dev       Date:  1997-09-15       Impact factor: 11.361

Review 5.  Initiation of protein synthesis in eukaryotes.

Authors:  H O Voorma; A A Thomas; H A Van Heugten
Journal:  Mol Biol Rep       Date:  1994-05       Impact factor: 2.316

6.  Universally conserved translation initiation factors.

Authors:  N C Kyrpides; C R Woese
Journal:  Proc Natl Acad Sci U S A       Date:  1998-01-06       Impact factor: 11.205

7.  Phosphorylation of mammalian translation initiation factor 5 (eIF5) in vitro and in vivo.

Authors:  Romit Majumdar; Amitabha Bandyopadhyay; Haiteng Deng; Umadas Maitra
Journal:  Nucleic Acids Res       Date:  2002-03-01       Impact factor: 16.971

8.  A novel zinc finger protein encoded by a couch potato homologue from Solanum tuberosum enables a sucrose transport-deficient yeast strain to grow on sucrose.

Authors:  C Kühn; W B Frommer
Journal:  Mol Gen Genet       Date:  1995-06-25

9.  Multidomain organization of eukaryotic guanine nucleotide exchange translation initiation factor eIF-2B subunits revealed by analysis of conserved sequence motifs.

Authors:  E V Koonin
Journal:  Protein Sci       Date:  1995-08       Impact factor: 6.725
  9 in total

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