Literature DB >> 8417033

Development and use of species-specific oligonucleotide probes for differentiation of Streptococcus uberis and Streptococcus parauberis.

R W Bentley1, J A Leigh, M D Collins.   

Abstract

Oligonucleotide probes specific for 16S rRNA and capable of differentiating Streptococcus uberis and S. parauberis from each other and other esculin-hydrolyzing streptococci were developed. Use of a mini-RNA extraction technique for gram-positive cocci associated with bovine mastitis has allowed the probes to be used for identification of esculin-hydrolyzing streptococci from two dairy herds at the Institute for Animal Health, Compton, United Kingdom. One hundred seventy-nine of 206 isolates were identified as S. uberis, 3 were identified as S. parauberis, and 24 were not identified. Isolates not identified by the probes were tested biochemically and found to be mainly Enterococcus faecium, E. faecalis, or S. bovis.

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Year:  1993        PMID： 8417033      PMCID： PMC262621          DOI： 10.1128/jcm.31.1.57-60.1993

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  15 in total

1.  DNA fingerprinting of Streptococcus uberis based on polymorphism of DNA encoding rRNA.

Authors:  A M Williams; M D Collins
Journal:  Lett Appl Microbiol       Date:  1991-01       Impact factor: 2.858

2.  Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences.

Authors:  R W Bentley; J A Leigh; M D Collins
Journal:  Int J Syst Bacteriol       Date:  1991-10

3.  Phylogenetic group-specific oligodeoxynucleotide probes for identification of single microbial cells.

Authors:  S J Giovannoni; E F DeLong; G J Olsen; N R Pace
Journal:  J Bacteriol       Date:  1988-02       Impact factor: 3.490

4.  Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

Authors:  P Chomczynski; N Sacchi
Journal:  Anal Biochem       Date:  1987-04       Impact factor: 3.365

5.  [Biochemical and serological characters of Streptococcus uberis].

Authors:  M Roguinsky
Journal:  Ann Inst Pasteur (Paris)       Date:  1971-02

6.  Differentiation of Streptococcus uberis from Streptococcus parauberis by polymerase chain reaction and restriction fragment length polymorphism analysis of 16S ribosomal DNA.

Authors:  B M Jayarao; J J Doré; G A Baumbach; K R Matthews; S P Oliver
Journal:  J Clin Microbiol       Date:  1991-12       Impact factor: 5.948

7.  Detection of sickle cell beta S-globin allele by hybridization with synthetic oligonucleotides.

Authors:  B J Conner; A A Reyes; C Morin; K Itakura; R L Teplitz; R B Wallace
Journal:  Proc Natl Acad Sci U S A       Date:  1983-01       Impact factor: 11.205

8.  Molecular taxonomic studies on Streptococcus uberis types I and II. Description of Streptococcus parauberis sp. nov.

Authors:  A M Williams; M D Collins
Journal:  J Appl Bacteriol       Date:  1990-05

9.  Nonradioactive labeling of oligonucleotides in vitro with the hapten digoxigenin by tailing with terminal transferase.

Authors:  G G Schmitz; T Walter; R Seibl; C Kessler
Journal:  Anal Biochem       Date:  1991-01       Impact factor: 3.365

10.  Rapid differentiation of streptococci isolated from cows with mastitis.

Authors:  P Schaufuss; C Lämmler; H Blobel
Journal:  J Clin Microbiol       Date:  1986-12       Impact factor: 5.948
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  5 in total

1.  Identification of major Streptococcal species by rrn-amplified ribosomal DNA restriction analysis.

Authors:  Laurent Schlegel; Francine Grimont; Patrick A D Grimont; Anne Bouvet
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

2.  Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis.

Authors:  A A Hassan; I U Khan; A Abdulmawjood; C Lämmler
Journal:  J Clin Microbiol       Date:  2001-04       Impact factor: 5.948

3.  Development of PCR-based hybridization protocol for identification of streptococcal species.

Authors:  R W Bentley; J A Leigh
Journal:  J Clin Microbiol       Date:  1995-05       Impact factor: 5.948

4.  Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer.

Authors:  B R Berridge; J D Fuller; J de Azavedo; D E Low; H Bercovier; P F Frelier
Journal:  J Clin Microbiol       Date:  1998-09       Impact factor: 5.948

Review 5.  What happened to the streptococci: overview of taxonomic and nomenclature changes.

Authors:  Richard Facklam
Journal:  Clin Microbiol Rev       Date:  2002-10       Impact factor: 26.132
  5 in total

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