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Literature DB >> 11283100

Evaluation of PCR methods for rapid identification and differentiation of Streptococcus uberis and Streptococcus parauberis.

A A Hassan¹, I U Khan, A Abdulmawjood, C Lämmler.

Abstract

Streptococcus uberis and Streptococcus parauberis reference strains and isolates obtained from routine diagnostics were investigated by PCR with oligonucleotide primers designed according to species-specific parts of the 16S rRNA gene, the 23S rRNA gene, and the 16S-23S rRNA intergenic spacer region of both species. All three primer pairs allowed an identification of 67 isolates as S. uberis and 4 isolates as S. parauberis.

Entities: Chemical Species

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Year: 2001 PMID： 11283100 PMCID： PMC87983 DOI： 10.1128/JCM.39.4.1618-1621.2001

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

15 in total

1. Amplification of 16S ribosomal RNA gene sequences for the identification of streptococci of Lancefield group B.

Authors: A Abdulmawjood; C Lämmler
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2. Development of gene probes for the specific identification of Streptococcus uberis and Streptococcus parauberis based upon large subunit rRNA gene sequences.

Authors: N M Harland; J A Leigh; M D Collins
Journal: J Appl Bacteriol Date: 1993-05

3. Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences.

Authors: R W Bentley; J A Leigh; M D Collins
Journal: Int J Syst Bacteriol Date: 1991-10

4. Determination of intraspecies variations of the V2 region of the 16S rRNA gene of Streptococcus equi subsp. zooepidemicus.

Authors: A Abdulmawjood; C H Lämmler
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5. Differentiation of Streptococcus uberis from Streptococcus parauberis by polymerase chain reaction and restriction fragment length polymorphism analysis of 16S ribosomal DNA.

Authors: B M Jayarao; J J Doré; G A Baumbach; K R Matthews; S P Oliver
Journal: J Clin Microbiol Date: 1991-12 Impact factor: 5.948

6. Characterization of the Lancefield group C streptococcus 16S-23S RNA gene intergenic spacer and its potential for identification and sub-specific typing.

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7. Development of PCR-based hybridization protocol for identification of streptococcal species.

Authors: R W Bentley; J A Leigh
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8. Restriction fragment length polymorphism analysis of 16S ribosomal DNA of Streptococcus and Enterococcus species of bovine origin.

Authors: B M Jayarao; J J Doré; S P Oliver
Journal: J Clin Microbiol Date: 1992-09 Impact factor: 5.948

9. Biochemical and serological properties of Streptococcus uberis.

Authors: C Lämmler
Journal: Zentralbl Veterinarmed B Date: 1991-12

10. Development and use of species-specific oligonucleotide probes for differentiation of Streptococcus uberis and Streptococcus parauberis.

Authors: R W Bentley; J A Leigh; M D Collins
Journal: J Clin Microbiol Date: 1993-01 Impact factor: 5.948

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4. Identification of Streptococcus uberis multilocus sequence types highly associated with mastitis.

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5. The hyaluronic acid capsule of Streptococcus uberis is not required for the development of infection and clinical mastitis.

Authors: Terence R Field; Philip N Ward; Lars H Pedersen; James A Leigh
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6. First insights into the evolution of Streptococcus uberis: a multilocus sequence typing scheme that enables investigation of its population biology.

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