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Literature DB >> 8414987

Trypanosoma brucei minicircles encode multiple guide RNAs which can direct editing of extensively overlapping sequences.

R A Corell¹, J E Feagin, G R Riley, T Strickland, J A Guderian, P J Myler, K Stuart.

Abstract

Small guide RNAs (gRNAs) may direct RNA editing in kinetoplastid mitochondria. We have characterized multiple gRNA genes from Trypanosoma brucei (EATRO 164), that can specify up to 30% of the editing of the COIII, ND7, ND8, and A6 mRNAs and we have also found that the non-translated region of edited COIII mRNA of strain (EATRO 164) differs from that of another strain. Several of the gRNAs specify overlapping regions of the same mRNA often specifying sequence beyond that required for an anchor duplex with the next gRNA. Some gRNAs have different sequence but specify identical editing of the same region of mRNA. These data indicate a complex gRNA population and consequent complex pattern of editing in T. brucei.

Entities: Disease Species

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Year: 1993 PMID： 8414987 PMCID： PMC310066 DOI： 10.1093/nar/21.18.4313

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

35 in total

1. The polarity of editing within a multiple gRNA-mediated domain is due to formation of anchors for upstream gRNAs by downstream editing.

Authors: D A Maslov; L Simpson
Journal: Cell Date: 1992-08-07 Impact factor: 41.582

10. Structure of the Trypanosoma brucei p22 protein, a cytochrome oxidase subunit II-specific RNA-editing accessory factor.

Authors: Mareen Sprehe; John C Fisk; Sarah M McEvoy; Laurie K Read; Maria A Schumacher
Journal: J Biol Chem Date: 2010-04-14 Impact factor: 5.157

Trypanosoma brucei minicircles encode multiple guide RNAs which can direct editing of extensively overlapping sequences.

1. The polarity of editing within a multiple gRNA-mediated domain is due to formation of anchors for upstream gRNAs by downstream editing.

2. Cycles of progressive realignment of gRNA with mRNA in RNA editing.

3. Trypanosoma equiperdum minicircles encode three distinct primary transcripts which exhibit guide RNA characteristics.

4. Identification of negative-strand complements to cytochrome oxidase subunit III RNA in Trypanosoma brucei.

5. Creation of AUG initiation codons by addition of uridines within cytochrome b transcripts of kinetoplastids.

Review 6. Trypanosomatids: mitochondrial RNA editing.

7. PCR-based cDNA library construction: general cDNA libraries at the level of a few cells.

8. Mitochondrial DNA of an African trypanosome.

9. The IsTaR 1 serodeme of Trypanosoma brucei: development of a new serodeme.

10. Extensive editing of the cytochrome c oxidase III transcript in Trypanosoma brucei.

1. Guide RNAs of the recently isolated LEM125 strain of Leishmania tarentolae: an unexpected complexity.

Review 2. Unexplained complexity of the mitochondrial genome and transcriptome in kinetoplastid flagellates.

3. Editing domains of Trypanosoma brucei mitochondrial RNAs identified by secondary structure.

4. Editing of Trypanosoma brucei maxicircle CR5 mRNA generates variable carboxy terminal predicted protein sequences.

5. Complexes from Trypanosoma brucei that exhibit deletion editing and other editing-associated properties.

Review 6. The mechanism of U insertion/deletion RNA editing in kinetoplastid mitochondria.

7. Guide RNA-directed uridine insertion RNA editing in vitro.

Review 8. RNA editing in kinetoplastid protozoa.

9. Complete set of mitochondrial pan-edited mRNAs in Leishmania mexicana amazonensis LV78.

10. Structure of the Trypanosoma brucei p22 protein, a cytochrome oxidase subunit II-specific RNA-editing accessory factor.