Literature DB >> 8265574

Strings of contiguous modified pentanucleotides with increased DNA-binding affinity can be used for DNA sequencing by primer walking.

T Azhikina1, S Veselovskaya, V Myasnikov, V Potapov, O Ermolayeva, E Sverdlov.   

Abstract

Modified oligonucleotides containing 5-methylcytidine and/or 2-aminoadenosine form tighter hybrids with DNA and are, therefore, more efficient primers for DNA sequencing as compared to their natural counterparts. Strings of contiguous modified pentanucleotides can be used for DNA sequencing by primer walking.

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Year:  1993        PMID： 8265574      PMCID： PMC48003          DOI： 10.1073/pnas.90.24.11460

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  10 in total

1.  A strategy for high-volume sequencing of cosmid DNAs: random and directed priming with a library of oligonucleotides.

Authors:  F W Studier
Journal:  Proc Natl Acad Sci U S A       Date:  1989-09       Impact factor: 11.205

2.  Thermodynamic studies of base pairing involving 2,6-diaminopurine.

Authors:  C Cheong; I Tinoco; A Chollet
Journal:  Nucleic Acids Res       Date:  1988-06-10       Impact factor: 16.971

3.  Effect of 5-bromo- and 5-methyldeoxycytosine on duplex stability and discrimination of the NotI octadeoxynucleotide. Quantitative measurements using thin-layer chromatography.

Authors:  J D Hoheisel; A G Craig; H Lehrach
Journal:  J Biol Chem       Date:  1990-09-25       Impact factor: 5.157

4.  [Oligonucleotides forming highly stable, specific duplexes, their use as primers in sequencing and in the polymerase chain reaction].

Authors:  T L Azhikina; Iu O Shevchenko; Iu B Lebedev; S V Veselovskaia; V A Miasnikov; V K Potapov; E D Sverdlov
Journal:  Dokl Akad Nauk       Date:  1993-06

5.  The filamentous phage (Ff) as vectors for recombinant DNA--a review.

Authors:  N D Zinder; J D Boeke
Journal:  Gene       Date:  1982 Jul-Aug       Impact factor: 3.688

6.  DNA sequence analysis with a modified bacteriophage T7 DNA polymerase.

Authors:  S Tabor; C C Richardson
Journal:  Proc Natl Acad Sci U S A       Date:  1987-07       Impact factor: 11.205

7.  In situ activation of bis-dialkylaminophosphines--a new method for synthesizing deoxyoligonucleotides on polymer supports.

Authors:  A D Barone; J Y Tang; M H Caruthers
Journal:  Nucleic Acids Res       Date:  1984-05-25       Impact factor: 16.971

8.  Dialkylformamidines: depurination resistant N6-protecting group for deoxyadenosine.

Authors:  B C Froehler; M D Matteucci
Journal:  Nucleic Acids Res       Date:  1983-11-25       Impact factor: 16.971

9.  DNA sequencing by primer walking with strings of contiguous hexamers.

Authors:  J Kieleczawa; J J Dunn; F W Studier
Journal:  Science       Date:  1992-12-11       Impact factor: 47.728

10.  Synthesis of 4-triazolopyrimidinone nucleotide and its application in synthesis of 5-methylcytosine-containing oligodeoxyribonucleotides.

Authors:  W L Sung
Journal:  Nucleic Acids Res       Date:  1981-11-25       Impact factor: 16.971
  10 in total
  3 in total

1.  The thermodynamic advantage of DNA oligonucleotide 'stacking hybridization' reactions: energetics of a DNA nick.

Authors:  M J Lane; T Paner; I Kashin; B D Faldasz; B Li; F J Gallo; A S Benight
Journal:  Nucleic Acids Res       Date:  1997-02-01       Impact factor: 16.971

2.  DNA sequencing using differential extension with nucleotide subsets (DENS).

Authors:  M C Raja; D Zevin-Sonkin; J Shwartzburd; T A Rozovskaya; I A Sobolev; O Chertkov; V Ramanathan; L Lvovsky; L E Ulanovsky
Journal:  Nucleic Acids Res       Date:  1997-02-15       Impact factor: 16.971

3.  Merging Two Strategies for Mixed-Sequence Recognition of Double-Stranded DNA: Pseudocomplementary Invader Probes.

Authors:  Brooke A Anderson; Patrick J Hrdlicka
Journal:  J Org Chem       Date:  2016-03-30       Impact factor: 4.354
  3 in total

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