Literature DB >> 8031087

In situ detection of hepatitis A virus in cell cultures and shellfish tissues.

J L Romalde1, M K Estes, G Szücs, R L Atmar, C M Woodley, T G Metcalf.   

Abstract

An in situ transcription method was developed to detect hepatitis A virus RNA in both cell cultures and shellfish tissues. Radiolabeled cDNA copies were synthesized in situ by reverse transcriptase-directed transcription after annealing with a specific primer to the viral RNA. Both tritium (3H) and 35S were useful in the in situ transcription reaction, but the use of 3H resulted in a lower background and finer detail in the localization of viral particles. Application of the method to different organs of oysters which had bioaccumulated hepatitis A virus allowed the first in situ localization of the virus, specifically in stomach and hepatopancreatic tissues.

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Year:  1994        PMID： 8031087      PMCID： PMC201581          DOI： 10.1128/aem.60.6.1921-1926.1994

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  18 in total

1.  Human enteroviruses in oysters and their overlying waters.

Authors:  S M Goyal; C P Gerba; J L Melnick
Journal:  Appl Environ Microbiol       Date:  1979-03       Impact factor: 4.792

2.  Detection of hepatitis A antigen by immunofluorescence.

Authors:  L R Mathiesen; S M Feinstone; R H Purcell; J A Wagner
Journal:  Infect Immun       Date:  1977-11       Impact factor: 3.441

3.  Production of cytopathology in FRhK-4 cells by BS-C-1-passaged hepatitis A virus.

Authors:  A M Nasser; T G Metcalf
Journal:  Appl Environ Microbiol       Date:  1987-12       Impact factor: 4.792

4.  Detection of hepatitis A virus and antibody by solid-phase radioimmunoassay and enzyme-linked immunosorbent assay with monoclonal antibodies.

Authors:  A G Coulepis; M F Veale; A MacGregor; M Kornitschuk; I D Gust
Journal:  J Clin Microbiol       Date:  1985-07       Impact factor: 5.948

5.  Widespread outbreaks of clam- and oyster-associated gastroenteritis. Role of Norwalk virus.

Authors:  D L Morse; J J Guzewich; J P Hanrahan; R Stricof; M Shayegani; R Deibel; J C Grabau; N A Nowak; J E Herrmann; G Cukor
Journal:  N Engl J Med       Date:  1986-03-13       Impact factor: 91.245

6.  Detection of hepatitis A virus in seeded estuarine samples by hybridization with cDNA probes.

Authors:  X Jiang; M K Estes; T G Metcalf; J L Melnick
Journal:  Appl Environ Microbiol       Date:  1986-10       Impact factor: 4.792

7.  Improved method and test strategy for recovery of enteric viruses from shellfish.

Authors:  T G Metcalf; E Moulton; D Eckerson
Journal:  Appl Environ Microbiol       Date:  1980-01       Impact factor: 4.792

8.  A new method of RNA preparation for detection of hepatitis A virus in environmental samples by the polymerase chain reaction.

Authors:  F Yang; X Xu
Journal:  J Virol Methods       Date:  1993-06       Impact factor: 2.014

9.  Viral hepatitis.

Authors:  F Deinhardt; I D Gust
Journal:  Bull World Health Organ       Date:  1982       Impact factor: 9.408

10.  In situ transcription: specific synthesis of complementary DNA in fixed tissue sections.

Authors:  L H Tecott; J D Barchas; J H Eberwine
Journal:  Science       Date:  1988-06-17       Impact factor: 47.728
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  9 in total

1.  Virus-contaminated oysters: a three-month monitoring of oysters imported to Switzerland.

Authors:  Christian Beuret; Andreas Baumgartner; Jakob Schluep
Journal:  Appl Environ Microbiol       Date:  2003-04       Impact factor: 4.792

2.  Distribution of human virus contamination in shellfish from different growing areas in Greece, Spain, Sweden, and the United Kingdom.

Authors:  M Formiga-Cruz; G Tofiño-Quesada; S Bofill-Mas; D N Lees; K Henshilwood; A K Allard; A-C Conden-Hansson; B E Hernroth; A Vantarakis; A Tsibouxi; M Papapetropoulou; M D Furones; R Girones
Journal:  Appl Environ Microbiol       Date:  2002-12       Impact factor: 4.792

3.  Use of rotavirus virus-like particles as surrogates to evaluate virus persistence in shellfish.

Authors:  Fabienne Loisy; Robert L Atmar; Jean-Claude Le Saux; Jean Cohen; Marie-Paule Caprais; Monique Pommepuy; Françoise S Le Guyader
Journal:  Appl Environ Microbiol       Date:  2005-10       Impact factor: 4.792

4.  Collaborative evaluation of a method for the detection of Norwalk virus in shellfish tissues by PCR.

Authors:  R L Atmar; F H Neill; C M Woodley; R Manger; G S Fout; W Burkhardt; L Leja; E R McGovern; F Le Guyader; T G Metcalf; M K Estes
Journal:  Appl Environ Microbiol       Date:  1996-01       Impact factor: 4.792

5.  Three-year study to assess human enteric viruses in shellfish.

Authors:  F Le Guyader; L Haugarreau; L Miossec; E Dubois; M Pommepuy
Journal:  Appl Environ Microbiol       Date:  2000-08       Impact factor: 4.792

6.  Detection of both hepatitis A virus and Norwalk-like virus in imported clams associated with food-borne illness.

Authors:  David H Kingsley; Gloria K Meade; Gary P Richards
Journal:  Appl Environ Microbiol       Date:  2002-08       Impact factor: 4.792

7.  Reverse transcriptase PCR detection of astrovirus, hepatitis A virus, and poliovirus in experimentally contaminated mussels: comparison of several extraction and concentration methods.

Authors:  O Traore; C Arnal; B Mignotte; A Maul; H Laveran; S Billaudel; L Schwartzbrod
Journal:  Appl Environ Microbiol       Date:  1998-08       Impact factor: 4.792

8.  Detection of Norwalk virus and hepatitis A virus in shellfish tissues with the PCR.

Authors:  R L Atmar; F H Neill; J L Romalde; F Le Guyader; C M Woodley; T G Metcalf; M K Estes
Journal:  Appl Environ Microbiol       Date:  1995-08       Impact factor: 4.792

Review 9.  A Comprehensive Review on Human Aichi Virus.

Authors:  Enrique Rivadulla; Jesús L Romalde
Journal:  Virol Sin       Date:  2020-04-27       Impact factor: 4.327
  9 in total

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