Literature DB >> 2829721

Production of cytopathology in FRhK-4 cells by BS-C-1-passaged hepatitis A virus.

A M Nasser1, T G Metcalf.   

Abstract

Cytopathic effects were produced in fetal rhesus monkey kidney (FRhK-4) cells 7 days postinfection by a serially BS-C-1-passaged strain of hepatitis A virus. Typical enterovirus cytopathology was produced by the HM-175 strain after 15 passages at 7-day intervals in BS-C-1 cells. No cytopathic effects were obtained after neutralization of virus with human anti-hepatitis A virus immunoglobulin G. Normal human serum had no effect on development of cytopathology. Maximum antigen and cDNA probe-based hybridization activity were associated with a CsCl gradient fraction having a density of 1.34 g/cm3. Large quantities of 27- to 30-nm virions typical of hepatitis A virus were associated with the same fraction. These data led to the conclusion that the observed cytopathology was caused by hepatitis A virus.

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Year:  1987        PMID： 2829721      PMCID： PMC204231          DOI： 10.1128/aem.53.12.2967-2971.1987

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  21 in total

1.  Electron microscopic counting of virus particles by sedimentation on aluminized grids.

Authors:  K O SMITH; J L MELNICK
Journal:  J Immunol       Date:  1962-08       Impact factor: 5.422

2.  Propagation of human hepatitis A virus in cell culture in vitro.

Authors:  P J Provost; M R Hilleman
Journal:  Proc Soc Exp Biol Med       Date:  1979-02

3.  Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors:  P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal:  J Mol Biol       Date:  1977-06-15       Impact factor: 5.469

4.  Propagation of hepatitis A virus in human embryo fibroblasts.

Authors:  V Gauss-Müller; G G Frösner; F Deinhardt
Journal:  J Med Virol       Date:  1981       Impact factor: 2.327

5.  Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenyl glycoluril (Iodogen).

Authors:  P R Salacinski; C McLean; J E Sykes; V V Clement-Jones; P J Lowry
Journal:  Anal Biochem       Date:  1981-10       Impact factor: 3.365

6.  Detection of hepatitis A viral antigen by radioimmunoassay.

Authors:  F B Hollinger; D W Bradley; J E Maynard; G R Dreesman; J L Melnick
Journal:  J Immunol       Date:  1975-11       Impact factor: 5.422

7.  Propagation of human hepatitis A virus in a hepatoma cell line.

Authors:  G G Frösner; F Deinhardt; R Scheid; V Gauss-Müller; N Holmes; V Messelberger; G Siegl; J J Alexander
Journal:  Infection       Date:  1979       Impact factor: 3.553

8.  Hepatitis A-virus in cell culture: I. propagation of different hepatitis A-virus isolates in a fetal rhesus monkey kidney cell line (Frhk-4).

Authors:  B Flehmig
Journal:  Med Microbiol Immunol       Date:  1980       Impact factor: 3.402

9.  Replication of mengovirus. I. Effect on synthesis of macromolecules by host cell.

Authors:  P G Plagemann; H E Swim
Journal:  J Bacteriol       Date:  1966-06       Impact factor: 3.490

10.  Propagation of human hepatitis A virus in African green monkey kidney cell culture: primary isolation and serial passage.

Authors:  R J Daemer; S M Feinstone; I D Gust; R H Purcell
Journal:  Infect Immun       Date:  1981-04       Impact factor: 3.441
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  11 in total

1.  Mutational characteristics in consecutive passage of rapidly replicating variants of hepatitis A virus strain H2 during cell culture adaptation.

Authors:  Ning-Zhu Hu; Yun-Zhang Hu; Hai-Jing Shi; Guo-Dong Liu; Su Qu
Journal:  World J Gastroenterol       Date:  2002-10       Impact factor: 5.742

2.  Determinants in 3Dpol modulate the rate of growth of hepatitis A virus.

Authors:  Krishnamurthy Konduru; Gerardo G Kaplan
Journal:  J Virol       Date:  2010-06-09       Impact factor: 5.103

3.  Real-time nucleic acid sequence-based amplification assay for detection of hepatitis A virus.

Authors:  Khaled H Abd el-Galil; M A el-Sokkary; S M Kheira; Andre M Salazar; Marylynn V Yates; Wilfred Chen; Ashok Mulchandani
Journal:  Appl Environ Microbiol       Date:  2005-11       Impact factor: 4.792

Review 4.  Diagnosis of hepatitis a virus infection: a molecular approach.

Authors:  Omana V Nainan; Guoliang Xia; Gilberto Vaughan; Harold S Margolis
Journal:  Clin Microbiol Rev       Date:  2006-01       Impact factor: 26.132

5.  Antigenic and genetic variation in cytopathic hepatitis A virus variants arising during persistent infection: evidence for genetic recombination.

Authors:  S M Lemon; P C Murphy; P A Shields; L H Ping; S M Feinstone; T Cromeans; R W Jansen
Journal:  J Virol       Date:  1991-04       Impact factor: 5.103

6.  A cytopathogenic, apoptosis-inducing variant of hepatitis A virus.

Authors:  K Brack; W Frings; A Dotzauer; A Vallbracht
Journal:  J Virol       Date:  1998-04       Impact factor: 5.103

7.  In situ hybridization for quantitative assay of infectious hepatitis A virus.

Authors:  J N Xi; M K Estes; T G Metcalf
Journal:  J Clin Microbiol       Date:  1989-05       Impact factor: 5.948

8.  Concentration and detection of hepatitis A virus and rotavirus from shellfish by hybridization tests.

Authors:  Y J Zhou; M K Estes; X Jiang; T G Metcalf
Journal:  Appl Environ Microbiol       Date:  1991-10       Impact factor: 4.792

9.  Detection of hepatitis A virus in Mercenaria mercenaria by coupled reverse transcription and polymerase chain reaction.

Authors:  B B Goswami; W H Koch; T A Cebula
Journal:  Appl Environ Microbiol       Date:  1993-09       Impact factor: 4.792

10.  In situ detection of hepatitis A virus in cell cultures and shellfish tissues.

Authors:  J L Romalde; M K Estes; G Szücs; R L Atmar; C M Woodley; T G Metcalf
Journal:  Appl Environ Microbiol       Date:  1994-06       Impact factor: 4.792
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