Literature DB >> 2991329

Detection of hepatitis A virus and antibody by solid-phase radioimmunoassay and enzyme-linked immunosorbent assay with monoclonal antibodies.

A G Coulepis, M F Veale, A MacGregor, M Kornitschuk, I D Gust.   

Abstract

Monoclonal antibodies (K3-2F2 and K3-4C8) raised against hepatitis A virus were used to develop a solid-phase radioimmunoassay and enzyme-linked immunosorbent assay for the detection of hepatitis A virus and antibody. Assays with this pair of monoclonal antibodies were compared in parallel with similarly constructed solid-phase radioimmunoassays and enzyme-linked immunosorbent assays in which human polyclonal serum was used. The monoclonal antibody assay proved to be more sensitive for the detection of hepatitis A virus from fecal specimens as well as for anti-hepatitis A virus immunoglobulin G (IgG) and IgM in sera.

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Year:  1985        PMID： 2991329      PMCID： PMC268334          DOI： 10.1128/jcm.22.1.119-124.1985

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  30 in total

1.  Picornaviridae: second report.

Authors: 
Journal:  Intervirology       Date:  1978       Impact factor: 1.763

2.  Comparison of a monoclonal antibody with a polyclonal serum in an enzyme-linked immunosorbent assay for detecting adenovirus.

Authors:  L J Anderson; E Godfrey; K McIntosh; J C Hierholzer
Journal:  J Clin Microbiol       Date:  1983-09       Impact factor: 5.948

3.  An investigation of the use of urease-antibody conjugates in enzyme immunoassays.

Authors:  H M Chandler; J C Cox; K Healey; A MacGregor; R R Premier; J G Hurrell
Journal:  J Immunol Methods       Date:  1982-09-17       Impact factor: 2.303

4.  Biophysical and biochemical characterization of hepatitis A virus.

Authors:  A G Coulepis; S A Locarnini; E G Westaway; G A Tannock; I D Gust
Journal:  Intervirology       Date:  1982       Impact factor: 1.763

5.  Identification and typing of herpes simplex viruses with monoclonal antibodies.

Authors:  N Balachandran; B Frame; M Chernesky; E Kraiselburd; Y Kouri; D Garcia; C Lavery; W E Rawls
Journal:  J Clin Microbiol       Date:  1982-07       Impact factor: 5.948

6.  Monoclonal antibodies against hepatitis A virus.

Authors:  A MacGregor; M Kornitschuk; J G Hurrell; N I Lehmann; A G Coulepis; S A Locarnini; I D Gust
Journal:  J Clin Microbiol       Date:  1983-11       Impact factor: 5.948

Review 7.  Taxonomic classification of hepatitis A virus.

Authors:  I D Gust; A G Coulepis; S M Feinstone; S A Locarnini; Y Moritsugu; R Najera; G Siegl
Journal:  Intervirology       Date:  1983       Impact factor: 1.763

8.  Microtiter solid-phase radioimmunoassay for hepatitis B antigen.

Authors:  R H Purcell; D C Wong; H J Alter; P V Holland
Journal:  Appl Microbiol       Date:  1973-10

9.  Hepatitis A: detection by immune electron microscopy of a viruslike antigen associated with acute illness.

Authors:  S M Feinstone; A Z Kapikian; R H Purceli
Journal:  Science       Date:  1973-12-07       Impact factor: 47.728

10.  Sensitivity of immunofluorescence with monoclonal antibodies for detection of Chlamydia trachomatis inclusions in cell culture.

Authors:  R S Stephens; C C Kuo; M R Tam
Journal:  J Clin Microbiol       Date:  1982-07       Impact factor: 5.948
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  7 in total

1.  Antigenic and immunogenic properties of recombinant hepatitis A virus 14S and 70S subviral particles.

Authors:  J T Stapleton; V Raina; P L Winokur; K Walters; D Klinzman; E Rosen; J H McLinden
Journal:  J Virol       Date:  1993-02       Impact factor: 5.103

2.  An A-ELISA to detect hepatitis A virus in estuarine samples.

Authors:  A M Nasser; T G Metcalf
Journal:  Appl Environ Microbiol       Date:  1987-05       Impact factor: 4.792

3.  Cellular changes associated with persistent hepatitis A infection in vitro.

Authors:  R E Kiernan; J A Marshall; A G Coulepis; D A Anderson; I D Gust
Journal:  Arch Virol       Date:  1987       Impact factor: 2.574

Review 4.  Clinical laboratory applications of monoclonal antibodies.

Authors:  W J Payne; D L Marshall; R K Shockley; W J Martin
Journal:  Clin Microbiol Rev       Date:  1988-07       Impact factor: 26.132

5.  Indirect immunofluorescence assay for the detection of hepatitis A virus-specific serum immunoglobulins.

Authors:  D A Anderson; A G Coulepis; M P Chenoweth; I D Gust
Journal:  J Clin Microbiol       Date:  1986-07       Impact factor: 5.948

6.  In situ detection of hepatitis A virus in cell cultures and shellfish tissues.

Authors:  J L Romalde; M K Estes; G Szücs; R L Atmar; C M Woodley; T G Metcalf
Journal:  Appl Environ Microbiol       Date:  1994-06       Impact factor: 4.792

Review 7.  Rapid and accurate viral diagnosis.

Authors:  M L Landry; D R Mayo; G D Hsiung
Journal:  Pharmacol Ther       Date:  1989       Impact factor: 12.310
  7 in total

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